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  • ThesisItemOpen Access
    Evaluation and quantification of fish food organisms and aquatic weeds of Ottu reservoir in Haryana
    (CCSHAU, Hisar, 2021-09) Mehta, Pragya; Gajender Singh
    The study was carried out in Ottu reservoir which is situated in Sirsa city of Haryana, India. Evaluation and quantification of fish food organisms and aquatic weeds of Ottu reservoir were monitored from March 2021 to June 2021. The evaluation of the planktonic population revealed that there were 36 genera of plankton recorded. Out of this 19 genera of phytoplankton belongs to class Bacillariophyceae (3 genera), Cyanophyceae (3 genera), Chlorophyceae (11 genera), Euglenophyceae (2 genera) and 17 genera of zooplankton belongs to class Copepoda (9 genera), Rotifera (2 genera), Cladocera (4 genera), and Protozoa (2 genera). In various months maximum, 123 species of phytoplankton were observed in site 2 and site 4 and Chlorophyceae found as dominant group while in zooplankton in various month site 4 showed maximum 391species of zooplankton and Cladocera group found as the dominant group. The highest quantity of plankton was observed in April while the minimum quantity was observed in March. Shannon and Weaver's diversity index for plankton found maximum in site 1(3.35) and minimum in site 4 (3.25). In site 1 and site 2 significant difference (P<0.05) was observed for the phytoplanktons groups present every month from March to June. However, a non-significant difference was observed in site 3 and site 4.Whilst the zooplankton group present in site 1, site 2, site 3, and site 4 from March to June was also found significant. A total of 7 species of aquatic weeds recorded 4species (Eicchornia crassipes, Lamnea minor, Pistia stratiotes and Nympahea alba) belongs to free floating aquatic weeds, 2 species (Ceratophyllum demersum , Hydrilla verticelleta) belongs to submerged aquatic weeds and 1 species (Typha latifolia) belongs to Emergent aquatic weeds. A total of 17 benthic species of benthos identified belongs to 2 phylum viz. annelida (3 species) and mollusca (14 species) and in mollusca 2 class viz. gastropoda and bivalve recorded. Maximum benthic fauna recorded in June.
  • ThesisItemOpen Access
    Physiological studies for thermo-tolerance in bread wheat genotypes
    (CCSHAU, 2019) Goyal, Alisha; Anita Kumari
    The present investigation was aimed to evaluate physiological traits for carbohydrates accumulation and mobilization and to find correlation between physiological traits and high temperature tolerance. Ten wheat genotypes viz DBW14 (Check), PBW781, PBW821, RAJ3765 (Check), RWP-2018-26, RWP-2018-30, RWP-2018-31, RWP-2018-32, WH1239 and WH730 (Check) were sown under TS and LS conditions during rabi season of 2018 - 19 at wheat research farm, Department of Genetics & Plant Breeding, CCS HAU, Hisar. The experiment was laid out in factorial RBD consisting of 6 rows of 3m length with a 20×5 cm spacing within rows & between plants, respectively. Data were recorded for various morphological traits including number of days to heading, number of days to maturity, plant height (cm), number of productive tillers per plant, spike length (cm), number of spikelets per spike, grain yield per plant (g), biological yield per plant (g), grain weight, number of grains per spike, 1000-grain weight (g), heat susceptibility index. Other studied parameters include normalized difference vegetation index, canopy temperature, transpiration rate, photosynthetic rate, stomatal conductance, chlorophyll content, cell membrane stability, free radical scavenging activity, osmotic potential, water soluble carbohydrates from different parts of stem, stem solidness, stem dry weight and grain growth rate. Out of ten genotypes, PBW821 was identified as thermo-tolerant on the basis of maximum NDVI & minimum CT at 90DAS, photosynthetic rate, transpiration rate, stomatal conductance, cell membrane stability, osmotic potential, water soluble carbohydrate, stem solidness and stem dry weight. PBW821 also showed maximum no. of spikelet/spike, no. of grains/spike, grain weight, biomass and grain yield under LS condition. Water soluble carbohydrate were found highly correlated with stem dry weight at maturity, stem solidness at 90DAS, stem dry weight at 90 DAS and biomass and stem solidness was also found highly correlated with biomass, stem dry weight at 90 DAS, grain growth rate and stem dry weight at maturity.
  • ThesisItemOpen Access
    Effect of planting season on seed yield, yield components and seed quality in maize hybrids (Zea mays L)
    (CCSHAU, 2018) Nidhi; Bhuker, Axay
    Four maize hybrids produced during rabi and kharif seasons were evaluated for their performance for different viability, vigour and yield parameters viz. standard germination, seedling length, seed weight, seed density, seedling dry weight, vigour index-I, vigour index-II, accelerated ageing test, electrical conductivity, tetrazolium test, dehydrogenase activity test, catalase activity test, peroxidase test, lipid peroxidation test, protein content, field emergence index, seedling establishment, number of leaves, number of cobs, cob length, plant height, days to 50 % tasseling, days to 50% silking, number of seeds per cob and seed yield to assess the effect of planting season on seed yield, yield components and seed quality in maize hybrids. The rabi season produced seed showed superiority over kharif season for all the seed quality and yield parameters. Among the hybrids, HQPM-5 had the top rank for almost all the vigour, viability and yield parameters in both the seasons (rabi and kharif) indicating its superiority over other hybrids. Standard germination was found significantly and positively correlated with most of the seed quality parameters except electrical conductivity and lipid peroxidation in both the seasons. Electrical conductivity and lipid peroxidation was found significantly and negatively associated with most of the parameters recorded in laboratory and field. On the basis of correlation study, it can be concluded that the viability test like tetrazolium test, electrical conductivity test, dehydrogenase activity test, accelerated ageing test, catalase activity test and lipid peroxidation test can be used as reliable predictor of standard germination in maize and rabi season can be considered as a better option for the production of quality seed in maize in Haryana
  • ThesisItemOpen Access
    Survey, isolation and identification of bacterial diseases of fresh water fishes in district Hisar, Haryana
    (CCSHAU, 2005) Dahiya, Tejpal; Sihag, R.C.;
    The present investigation was carried out to identify fish pathogens causing fish diseases in catla, rohu, mrigal, common carp, puthi and magur. A number of biochemical tests were carried out for identification of bacterial fish pathogens. The bacterial diseases were identified viz. epizootic ulcerative syndrome, fin and tail rot, hemorrhagic septicemia and vibriosis in three selected fish farms (Mirka, Satrod and SLR, Hansi) from July 2004 to March 2005. These diseases were reported in July, August, October and November. No disease was found in September, December, January, Febuary and March. Ten gram negative ( Aeromonas hydrophila, Enterobacter cloacae, E. gergoviae, Pseudomonas sp., P. pseudomallie, Klebsiella pneumoniae subsp. aerogenes, Providencia sp., Serratia marcescens, Vibrio anguillarum, V. alginolyticus) and two gram positive (Micrococcus roseus, Streptococcus gp Q1) bacterial pathogens were identified from infected fishes. In EUS, fungal hyphae were found penetrating deep into muscles in catla. In fish farm of Satrod, vibriosis was reported in magur. The bacterial pathogens (Vibrio anguillarum, V. alginolyticus) were isolated from the skin lesions. These bacteria are generally found in marine, brackish and rarely in fresh water. Deviation of optimal range of hydrobiological parameters led to infection in fishes resulting in to disease outbreaks.
  • ThesisItemOpen Access
    Plant regeneration studies in guar patta (Aloe vera L.)
    (CCSHAU, 2005) Saroha, Vandanna; Yadav, Neelam R.
    The present study was conducted to develop micropropagation system in Aloe vera L. and establishment of the regenerated plantlets in the soil. Shoot tip and leaf explants from 4-5 cm long offshoots were collected from field grown plants. These explants were cultured on 18 different MS modified media fortified with different concentrations and combinations of auxin, cytokinins and additives. MS medium supplemented with TDZ (1.0 mg/l) produced highest shoot formation response and further upscaling was done on MS medium with BA (2.0 mg/l) and IAA (0.2 mg/l). For shoot elongation, Tyrosine (50.0 mg/l) alongwith BA (5.0 mg/l) and adenine sulphate (80 mg/l) in MS medium produced 158.5 per cent increase in shoot length along with rooting. Leaf explants did not respond to shoot formation, however, 24.9 per cent callusing was observed on medium with 2,4-D (1.0 mg/l). The regenerated shoots showed 100 per cent rooting in all the six media tried for rooting, but longest roots (9.16 cm) and highest number of roots per shoot (13.22) were observed on medium fortified with paclobutrazol (0.5 mg/l) after 45 days of transfer. The regenerated plants were kept for hardening in water overnight and transferred to the pots with soil and vermiculite mixture (1:1). The rooting medium also affected the survival rate of the regenerated plants in soil. Highest survival rate was observed in paclobutrazol rooted plantlets. The regenerated plants were analysed for homogeneity through SDS-PAGE and all the plants showed similar soluble protein banding pattern. Promising variant has been recorded among plants regenerated on paclobutrazol (0.5 mg/l) containing medium. It also contained around four times more soluble protein and one and a half times more sapogenin content than the field grown plants. It showed thin cuticle, succulent and broad leaves. These plants are under observation for their utility in gel extraction.