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ThesisItem Open Access Molecular mapping of bacterial blight resistance gene, drought tolerant QTL(s) and genetic diversity analysis in clusterbean {Cyamopsis tetragonoloba(L) Taub}(CCSHAU, 2011) Bajaj, Anshu; Boora, K.S.The present investigation was carried out to screen genetic diversity using RAPD markers and to identify and map the QTL for resistance to bacterial blight and drought tolerance in clusterbean .The plant material was procured from Forage section . For bacterial blight, the resistant (HG 75) & susceptible(PNB) genotypes were selected & crossed to generate F3 population. The F3 population was used to map QTLs. For drought tolerance studies genotypes HG 563 (tolerant) & PNB (susceptible) were used to generate the mapping population (F 2:3).The genetic diversity studies were carried out in 48 genotypes of clusterbean using 130 RAPD primers and 97 primers produced amplification of 705 alleles ;out of which 644 were polymorphic. The similarity ranged from 0.11 to 0.97.The dendrogram grouped the genotypes into two major clusters at a similarity coefficient of 0.68. A variety FS277 was out grouped which is a forage variety. The genotype GP96 and F5277 were found to be most diverse (89%).The QTLs were identified for bacterial blight resistance using parental genotypes (HG75 & PNB) & their F3 population. The F 3 population was screened artificially for disease resistance along with the parent genotypes. Polymorphic primers were used to screen the F3 population & the major QTLs were identified for disease resistance using parental genotypes, HG75, PNB and their F 3 population. A total of 83 RAPD primers were used to screen the parental genotypes out of which nine primers were polymorphic. These primers were used to screen the F 3 population and the QTLs found were L19 , D1, AB7 and QLTY 3. Parental genotypes HG563 and PNB and their F2-3 genotypes were screened at field and net house for resistance to drought. Out of fifty genotypes, 12 were highly tolerant, 23 were moderately tolerant and 15 were susceptible to drought. The parents were screened with eighty seven primers out of which, sixteen were found polymorphic and nine QTLs were found linked to drought tolerance. Loci OPQ 20,OPD10, OPD14,OPQ 12,OPAC 8 and OPF 9 were major contributing QTLs and OPAC2,OPL3 and OPF17 are minor QTLs The diverse lines may be used for breeding programs to improve the production of agronomically superior genotypes of guar. The major QTLs for bacterial blight and drought tolerance may be used for introgression into agronomically superior genotypes of guar.ThesisItem Open Access Studies on the biology of onionwead (Asphodelus Tenuifolius cav.) and its herbicidal control in chickpea(College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 1984) Sahai, Bhagwan; Khanna, S. SThesisItem Open Access Assessing phosphate requirements based on intensity/capacity relationship in Haryana soils(College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 1984) Yadav, Hanuman Dass; Karwasra, S.P.S.ThesisItem Open Access Studies on the incidence and control of Sarcoptic ange in Buffaloes with some systemic Insecticides(College of Veterinary Sciences Chaudhary Charan Singh Haryana Agricultural University Hisar, 1984) Tikaram, Samuel Manly; Ruprah, N. SThesisItem Open Access Effect of Different Sources and levels of dietary fibre and protein on rumen metabolic profile in cattle and buffalou(College of Animal Sciences Chaudhary Charan Singh Haryana Agricultural University Hisar, 1984) Sangwan, D. C; Sangwan, VeenaThesisItem Open Access Studies on the efficacy of a bivalent vaccine against Marek's disease(College of Veterinary Sciences Chaudhary Charan Singh Haryana Agricultural University Hisar, 1984) Pruthi, Anil Kumar; Paul, R. KThesisItem Open Access Studies on the effect of irrigation and nitrogen levels on growth, yield, quality and water use efficiency of fodder oats (Avena sativa Linn.) Grown under Different Cutting Managements(College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 1984) Joon, Ram Karan; Singh, K. P.ThesisItem Open Access Studies on interaction between endoparasi tic nematodes and Rhizobium in' legumes and influence of fertilizers, phytotherapeutic substances and host on nematode development(College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 1984) Dalal, M. R; Bhatti, D. SThesisItem Open Access Genotoxic effects of certain agricultural chemicals in laboratory mice and cultured buffalo Lymphocytes(College of Basic Sciences and Humanities Chaudhary Charan Singh Haryana Agricultural University Hisar, 1984) Chaudhary, Indra; Chowdhury, J. B