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20052
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End date: 2006 × Type: Thesis × Rating Count: 5 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Survey, isolation and identification of bacterial diseases of fresh water fishes in district Hisar, Haryana
    (CCSHAU, 2005) Dahiya, Tejpal; Sihag, R.C.;
    The present investigation was carried out to identify fish pathogens causing fish diseases in catla, rohu, mrigal, common carp, puthi and magur. A number of biochemical tests were carried out for identification of bacterial fish pathogens. The bacterial diseases were identified viz. epizootic ulcerative syndrome, fin and tail rot, hemorrhagic septicemia and vibriosis in three selected fish farms (Mirka, Satrod and SLR, Hansi) from July 2004 to March 2005. These diseases were reported in July, August, October and November. No disease was found in September, December, January, Febuary and March. Ten gram negative ( Aeromonas hydrophila, Enterobacter cloacae, E. gergoviae, Pseudomonas sp., P. pseudomallie, Klebsiella pneumoniae subsp. aerogenes, Providencia sp., Serratia marcescens, Vibrio anguillarum, V. alginolyticus) and two gram positive (Micrococcus roseus, Streptococcus gp Q1) bacterial pathogens were identified from infected fishes. In EUS, fungal hyphae were found penetrating deep into muscles in catla. In fish farm of Satrod, vibriosis was reported in magur. The bacterial pathogens (Vibrio anguillarum, V. alginolyticus) were isolated from the skin lesions. These bacteria are generally found in marine, brackish and rarely in fresh water. Deviation of optimal range of hydrobiological parameters led to infection in fishes resulting in to disease outbreaks.
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    ThesisItemOpen Access
    Plant regeneration studies in guar patta (Aloe vera L.)
    (CCSHAU, 2005) Saroha, Vandanna; Yadav, Neelam R.
    The present study was conducted to develop micropropagation system in Aloe vera L. and establishment of the regenerated plantlets in the soil. Shoot tip and leaf explants from 4-5 cm long offshoots were collected from field grown plants. These explants were cultured on 18 different MS modified media fortified with different concentrations and combinations of auxin, cytokinins and additives. MS medium supplemented with TDZ (1.0 mg/l) produced highest shoot formation response and further upscaling was done on MS medium with BA (2.0 mg/l) and IAA (0.2 mg/l). For shoot elongation, Tyrosine (50.0 mg/l) alongwith BA (5.0 mg/l) and adenine sulphate (80 mg/l) in MS medium produced 158.5 per cent increase in shoot length along with rooting. Leaf explants did not respond to shoot formation, however, 24.9 per cent callusing was observed on medium with 2,4-D (1.0 mg/l). The regenerated shoots showed 100 per cent rooting in all the six media tried for rooting, but longest roots (9.16 cm) and highest number of roots per shoot (13.22) were observed on medium fortified with paclobutrazol (0.5 mg/l) after 45 days of transfer. The regenerated plants were kept for hardening in water overnight and transferred to the pots with soil and vermiculite mixture (1:1). The rooting medium also affected the survival rate of the regenerated plants in soil. Highest survival rate was observed in paclobutrazol rooted plantlets. The regenerated plants were analysed for homogeneity through SDS-PAGE and all the plants showed similar soluble protein banding pattern. Promising variant has been recorded among plants regenerated on paclobutrazol (0.5 mg/l) containing medium. It also contained around four times more soluble protein and one and a half times more sapogenin content than the field grown plants. It showed thin cuticle, succulent and broad leaves. These plants are under observation for their utility in gel extraction.