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Theses

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2017 - 20202
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Author: Sweety × End date: 2021 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Standardization of nitrogen and potassium for strawberry cv. Winter Dawn under greenhouse
    (CCSHAU, Hisar, 2020-10) Sweety; Godara, A.K
    A field experiment entitled “Standardization of nitrogen and potassium for strawberry cv. Winter Dawn under greenhouse" was conducted during the year 2018-19 and 2019-20 at hi-tech greenhouse of the Department of Horticulture, CCS Haryana Agricultural University, Hisar. The investigation composed of two experiments viz., (i) studies on the effect of different levels of nitrogen application in combination with phosphorous and potassium on growth, yield and quality of strawberry through fertigation and (ii) studies on the effect of different levels of potassium application in combination with nitrogen & phosphorous on growth, yield and quality of strawberry through fertigation. The data were recorded on plant height, number of leaves per plant, plant spread, crown diameter, emergence of first flower, fresh weight of plant, dry weight of plant, NPK status of plants and soil, number of fruits per plant, fresh weight of fruit, length and breadth of fruits, yield per plant, total soluble solids, titratable acidity, TSS/ acid ratio, ascorbic acid content and anthocyanin content. The recorded data were subjected to statistical analysis using Randomized Block Design. The strawberry plants were fertigated with nitrogen, potassium and phosphorous. N, P and K fertigation had significant effect on growth, yield and quality of strawberry. The results indicate that strawberry plants fertigated with 250 g N + 200 g P2O5 + 400 g K2O from 10 Oct to 20 Nov, 600 g N + 200 g P2O5 + 600 g K2O from 21 Nov to 20 Dec, 250 g N + 200 g P2O5 + 600 g K2O from 21 Dec to 20 Jan, 700 g N + 200 g P2O5 + 900 g K2O from 21 Jan to 28 Feb and 600 g N + 200 g P2O5 + 900 g K2O from 1 to 31 March significantly increased plant height, number of leaves, crown diameter, plant spread, NPK status of plants, NPK status of soil. Early emergence of first flower was observed with 600 g N + 200 g P2O5 + 600 g K2O, whereas, maximum number of fruits, fruit weight, yield, total soluble solids, ascorbic acids and anthocyanin content of strawberry with 250 g N + 200 g P2O5 + 600 g K2O from 21 Dec to 20 Jan, 700 g N + 200 g P2O5 + 900 g K2O from 21 Jan to 28 Feb and 600 g N + 200 g P2O5 + 900 g K2O from 1 to 31 March than the control.
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    ThesisItemOpen Access
    Transcriptome analysis for zinc and iron homeostasis and carbohydrate metabolism in rice (Oryza sativa L.)
    (CCSHAU, 2017) Sweety; Jain, Veena
    Biochemical and molecular changes in root and shoot tissues of four rice varieties (HKR47, PUSA1121, HBC19 and PAU201) were studied in response to varied iron and zinc concentrations (T1 = 0.1 mM Fe(II)-EDTA+ 0.06 mM ZnSO4.7H2O, T2 = 0.1 mM Fe(II)-EDTA+ 0.12 mM ZnSO4.7H2O, T3 = 0.1 mM Fe(II)-EDTA+ 0.48 mM ZnSO4.7H2O, T4 = 0.5 mM Fe(II)-EDTA+ 0.06 mM ZnSO4.7H2O, T5 = 0.5 mM Fe(II)-EDTA+ 0.12 mM ZnSO4.7H2O, T6 = 0.5 mM Fe(II)-EDTA+ 0.48 mM ZnSO4.7H2O and T7 = 0.05 mM Fe(II)-EDTA+ 0.48 mM ZnSO4.7H2O) at vegetative and reproductive stages. Fe content in roots, shoots and grains was maximum at T4 treatment while contrarily Zn content was highest at T7 treatment in all the four varieties. Highest iron and zinc in dehusked grains was recorded in HBC19 at all the treatments. The maximum specific enzymes’ activity of glycolytic pathway (Hexokinase, phosphofructokinase, fructose-1,6 bisphosphate aldolase, pyruvate kinase), Hexose monophosphate pathway (Glucose 6 phosphate dehydrogenase and 6-phosphate gluconate dehydrogenase) and tricarboxylic acid cycle (isocitrate dehydrogenase and malate dehydrogenase) was observed at T7 while at T4 treatment activity of these enzymes reduced the most. Reducing sugar and total soluble sugar content increased at T1, T4, T5 and T6 treatments as compared to control (T2); maximum increase being at T4. Expression of genes involved in iron and zinc homeostasis [Nicotinamine synthase 1 (OsNAS1), Nicotinamine synthase 2 (OsNAS2), Nicotinamine synthase 3 (OsNAS3), Iron-regulated transporter 1 (OsIRT1) and Yellow stripe like 2 (OsYSL2), zinc-regulated transporter (ZRT), iron-regulated transporter (IRT)-like protein 1 (OsZIP1), OsZIP3,OsZIP4 and OsZIP8] were analyzed at T2, T4 and T7 treatments at vegetative stage; reduced expression of OsNAS1,OsNAS2, OsIRT1and OsYSL2 was observed at T4 treatment in all the four varieties as compared to the control (T2) in both the tissues. Conversely, these genes had enhanced expressions at T7 treatment. OsNAS3, OsZIP1, OsZIP3, OsZIP4 and OsZIP8 genes contrarily showed enhancement in relative expressions at T4 treatment and reduction at T7 treatment as compared to control (T2). Differential gene expression under low iron or low zinc conditions suggests that poor uptake of zinc and iron via roots and further translocation to shoots may not be the reason for low iron and zinc in grains. All the four rice varieties had better growth, grain yield and yield attributes at T7 (low iron and high zinc) treatment as compared to control (T2). Higher activities of carbohydrate metabolism’s enzymes, plant growth and productivity under high zinc environment clearly indicate the importance of Zn for plants.