Theses

Browse

Search Results

Now showing 1 - 8 of 8

Open Access
Production, extraction and characterization of astaxanthin from microalgae
(Chaudhary Charan Singh Haryana Agricultural University hisar, 2022-09) Satish Kumar; Satish Kumar; Rakesh Kumar; Rakesh Kumar
In the present investigation, five microalgal isolates were retrieved from different regions of Himachal Pradesh and Haryana. Out of five, two isolates, PLM1 and PLM2, produced astaxanthin. Astaxanthin production potential of PLM1 was low; therefore, only PLM2 was selected for further studies. Based on molecular analysis, PLM1 and PLM2 were identified as novel species named Desmodesmus sp. nov. PLM1 and Desmodesmus sp. nov. PLM2. Optimized culture conditions include sodium nitrate (20 mM), glucose (3mM), potassium chloride (32mM), pH (7), incubation temperature (27°C), and 15 days of incubation time for the green and red stages. The media with optimized composition were re-named as Modified BG-11 or M-BG11 medium. After achieving maximum biomass yield, cells were harvested and inoculated in nitrogen, phosphorous limiting media supplemented with 0.2% NaCl and 4.4mM sodium acetate and incubated at 27℃ for 15 days under the illumination of the white light of intensity 3000 lux. After 15 days, microalgal red cyst cells from BG-11 and M-BG11 medium were harvested, lyophilized, stored, and directed for astaxanthin extraction using different solvents. Maximum astaxanthin extraction resulted from cells grown in M-BG-11 medium was 21.13 ppm using 4 M HCl. The extracted astaxanthin was confirmed for its presence using UHPLC, FTIR, and Raman spectroscopy. Astaxanthin extracted from Desmodesmus cells grown in two different media was evaluated for DPPH free radical scavenging activity. Maximum DPPH free radical scavenging activity (78.69%) and free hydroxyl radical activity (37.81%) were observed for astaxanthin extracted using 4M HCl. The lyophilized dried biomass was stored and evaluated for degradation at different temperatures, including -20°C, 4°C, 25°C, and room temperature. Maximum degradation (88%) was observed at room temperature after 20 weeks of storage for the BG11 medium. The red-light illumination enhanced the vegetative growth of Desmodesmus sp. PLM2, whereas blue-light illumination caused stress in Desmodesmus sp. PLM2, thereby triggering astaxanthin biosynthesis. The extracted astaxanthin was inhibiting the growth of Bacillus subtilis, Staphylococcus aureus, and E. coli.
Open Access
Characterization of phyllospheric bacteria for growth promotion of potato (Solanum tuberosum L.)
(CCSHAU, 2017) Satish Kumar; Rakesh Kumar
A total of forty six bacterial isolates were isolated from phyllosphere of five different crops (Cotton, Mung bean, Potato, Wheat, and Pearl millet). All the bacterial isolates were characterized for IAA production, Ammonia excretion, Antifungal activity, Nitrate reductase and Phosphate solubilization. All the isolates were producing IAA and highest IAA produced by isolate WHK2 (31.06 μg/ml). All the 46 isolates were capable of excreting ammonia and highest ammonia excretion was observed in isolate PMK3 (5.29 μg/ml). Out of 46 isolates, only 17 isolates showed antifungal activity against A. niger and R. solani. All the 46 isolates showed nitrate reductase activity and highest nitrate reductase activity was observed in isolate MUK1 (148.63 μg nitrite ml-1). A total of 15 bacterial isolates solubilized phosphate and highest phosphate solubilisation index was observed in isolate COJ4 (19.18). On the basis of plant growth promoting traits, total 7 isolates (POK3, PMK3, COJ4, MUK1, WHK2, WHN1 and WHN2) were selected for the pot house studies in potato (Kufri bahar var.). Different parameter such as total viable count of bacteria in phyllosphere, chlorophyll a & b content, ROS scavenging enzymes like superoxide dismutase, catalase and peroxidase were observed at different interval of time. Potato yield & its plant height were observed at 90 days after showing. Total phyllospheric viable count was maximum observed in foliar application of isolate PMK3 along with RDF i.e. treatment T15 (7.8 log no. cfu/g leaves) at 90 DAS. Maximum chlorophyll a content (1.80 mg/g fresh weight) and chlorophyll b content (1.38 mg/g fresh weight) was observed in isolate COJ4 along with RDF i.e. treatment T19 at 60 DAS. No fixed trend of any phyllospheric bacteria were observed on ROS activity in potato under pot house conditions. However maximum catalase ( 0.24 & 0.22 U/g fresh weight) observed in T4 Azotobacter chroococcum Mac27 (tuber treatment) along with 75% RDF at 30 and 60 DAS, maximum peroxidase (12.67 U/g fresh weight) observed in treatment T3 i.e. control RDF at 30 DAS and treatment T14 isolate PMK3 along with 75% RDF (12.52 U/g fresh weight) at 60 DAS, maximum superoxide dismutase (481.80 U/g fresh weight) observed in treatment T18 isolate COJ4 along with 75% RDF at 30 DAS and treatment T7 Azotobacter chroococcum Mac27 along with RDF (408.85 U/g fresh weight) at 60 DAS. Maximum plant height was observed with foliar application of isolate PMK3 along with RDF i.e. treatment T15 (36.0 cm). Highest potato yield was observed in foliar application of isolate PMK3 along with RDF i.e. treatment T15 which is 0.194 kg respectively. Among the seven bacterial isolates (POK3, PMK3, COJ4, MUK1, WHK2, WHN1 and WHN2) tested for plant growth promotion effect in potato crop, maximum plant growth promotion effect was observed with bacterial isolate PMK3 and COJ4. So, bacterial isolate PMK3 and COJ4 may be explored further for the field experiment.
Open Access
Role Of Earthworms In Relation To Conversion Of Soil Nutrients In Cultivated Soils
(Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2002) Satish Kumar; Sihag, R. C.
Open Access
Pathogenesis And Management Of Root Rot Disease Complex Of Okra [Abelmoschus Esculentus (L.) Moench]
(Chaudhary Charan Singh Haryana Agricultural University;Hisar, 2002) Satish Kumar; Tripathi, N.N.
Open Access
Comparative Studies Of Mango Fruit Of Different Cultivars For Processing
(Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2008) Satish Kumar; Godara, R. K.
Open Access
Studies on the effect of different blends on quality of RTS and nectar of aonla (Emblica officinalis Gaertn
(CCSHAU, 2004) Satish Kumar; Godara, R.K.
The investigation on the effect of different blends on quality of RTS and nectar of aonla were carried out during the year 2003-04 at Fruit Technology Laboratory, Department of Horticulture, CCS Haryana Agricultural University, Hisar. The present study were conducted to standardize the appropriate blending for different RTS and nectar of aonla. The preserved ready-to-serve and nectar were evaluated for different chemical changes at room temperature for three months. Best quality RTS beverage can be prepared by keeping 10% pulp, 15% TSS, 0.30% acidity with aonla (50%) + guava (50%) ratio. Amal (50%) + guava (50%) RTS beverage scored the highest points as compared to other treatments. In aonla-guava blended nectar, aonla (50%) + guava (50%) nectar was found best. It has excellent quality till 90 days of storage. In aonla-pineapple blended nectar, aonla (50%) + pineapple (50%) nectar was found best. It will scored the highest points as compared to other treatments. The pulp content was 20%, TSS 15% and acidity was 0.3% in both the nectars.
Open Access
Biomass Production and Nutrient Contents in 8-year old Poplar Plantation
(CCSHAU, 2006) Satish Kumar; Ravi Kumar
In order to study the “Biomass Production and Nutrient Contents in 8-year old Poplar Plantation” 8 years old popular plantations grown under 5x4 m, 10x2.5 m and 15x2.5 m spacing were selected. Seven trees in each spacing were felled for recording observations for biomass production and nutrient analysis. The total amount of biomass production was maximum in 5x4 m spacing, followed by 10x2.5 spacing and minimum in 15x2.5 m spacing. About 75 percent of biomass was contributed by stem only. The concentration of N, P and K was maximum in leaves at 15x2.5 m spacing and decreased with decrease in spacing. Secondary nutrients (Ca, Mg, S) also followed the similar trend. Their concentration was also maximum in leaves. Concentration of Zn, Fe, Cu and Mn was recorded maximum in 1.5x2.5 m spacing and decreased with increase in spacing. Zn and Cu concentration were maximum in leaves, while the concentration of Fe & Mn were higher in roots. The uptake of all nutrients analysed was maximum at 5 x4 m spacing and their major portion was accumulated in the stem in all the spacings. In soil concentration of available N, P, K, Ca, Mg, S & micronutrients were maximum at surface layer in all the spacing, which decreased with increase in sampling depth.
Open Access
Biomass Production and Nutrient Contents in 8-year old Poplar Plantation
(CCSHAU, 2006) Satish Kumar; Ravi Kumar
In order to study the “Biomass Production and Nutrient Contents in 8-year old Poplar Plantation” 8 years old popular plantations grown under 5x4 m, 10x2.5 m and 15x2.5 m spacing were selected. Seven trees in each spacing were felled for recording observations for biomass production and nutrient analysis. The total amount of biomass production was maximum in 5x4 m spacing, followed by 10x2.5 spacing and minimum in 15x2.5 m spacing. About 75 percent of biomass was contributed by stem only. The concentration of N, P and K was maximum in leaves at 15x2.5 m spacing and decreased with decrease in spacing. Secondary nutrients (Ca, Mg, S) also followed the similar trend. Their concentration was also maximum in leaves. Concentration of Zn, Fe, Cu and Mn was recorded maximum in 1.5x2.5 m spacing and decreased with increase in spacing. Zn and Cu concentration were maximum in leaves, while the concentration of Fe & Mn were higher in roots. The uptake of all nutrients analysed was maximum at 5 x4 m spacing and their major portion was accumulated in the stem in all the spacings. In soil concentration of available N, P, K, Ca, Mg, S & micronutrients were maximum at surface layer in all the spacing, which decreased with increase in sampling depth.

Theses

Browse

Thesis Type

Type

Author

Date

Subject

Search Results