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2004 - 200932010 - 20171
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Author: Satish Kumar × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Characterization of phyllospheric bacteria for growth promotion of potato (Solanum tuberosum L.)
    (CCSHAU, 2017) Satish Kumar; Rakesh Kumar
    A total of forty six bacterial isolates were isolated from phyllosphere of five different crops (Cotton, Mung bean, Potato, Wheat, and Pearl millet). All the bacterial isolates were characterized for IAA production, Ammonia excretion, Antifungal activity, Nitrate reductase and Phosphate solubilization. All the isolates were producing IAA and highest IAA produced by isolate WHK2 (31.06 μg/ml). All the 46 isolates were capable of excreting ammonia and highest ammonia excretion was observed in isolate PMK3 (5.29 μg/ml). Out of 46 isolates, only 17 isolates showed antifungal activity against A. niger and R. solani. All the 46 isolates showed nitrate reductase activity and highest nitrate reductase activity was observed in isolate MUK1 (148.63 μg nitrite ml-1). A total of 15 bacterial isolates solubilized phosphate and highest phosphate solubilisation index was observed in isolate COJ4 (19.18). On the basis of plant growth promoting traits, total 7 isolates (POK3, PMK3, COJ4, MUK1, WHK2, WHN1 and WHN2) were selected for the pot house studies in potato (Kufri bahar var.). Different parameter such as total viable count of bacteria in phyllosphere, chlorophyll a & b content, ROS scavenging enzymes like superoxide dismutase, catalase and peroxidase were observed at different interval of time. Potato yield & its plant height were observed at 90 days after showing. Total phyllospheric viable count was maximum observed in foliar application of isolate PMK3 along with RDF i.e. treatment T15 (7.8 log no. cfu/g leaves) at 90 DAS. Maximum chlorophyll a content (1.80 mg/g fresh weight) and chlorophyll b content (1.38 mg/g fresh weight) was observed in isolate COJ4 along with RDF i.e. treatment T19 at 60 DAS. No fixed trend of any phyllospheric bacteria were observed on ROS activity in potato under pot house conditions. However maximum catalase ( 0.24 & 0.22 U/g fresh weight) observed in T4 Azotobacter chroococcum Mac27 (tuber treatment) along with 75% RDF at 30 and 60 DAS, maximum peroxidase (12.67 U/g fresh weight) observed in treatment T3 i.e. control RDF at 30 DAS and treatment T14 isolate PMK3 along with 75% RDF (12.52 U/g fresh weight) at 60 DAS, maximum superoxide dismutase (481.80 U/g fresh weight) observed in treatment T18 isolate COJ4 along with 75% RDF at 30 DAS and treatment T7 Azotobacter chroococcum Mac27 along with RDF (408.85 U/g fresh weight) at 60 DAS. Maximum plant height was observed with foliar application of isolate PMK3 along with RDF i.e. treatment T15 (36.0 cm). Highest potato yield was observed in foliar application of isolate PMK3 along with RDF i.e. treatment T15 which is 0.194 kg respectively. Among the seven bacterial isolates (POK3, PMK3, COJ4, MUK1, WHK2, WHN1 and WHN2) tested for plant growth promotion effect in potato crop, maximum plant growth promotion effect was observed with bacterial isolate PMK3 and COJ4. So, bacterial isolate PMK3 and COJ4 may be explored further for the field experiment.
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    ThesisItemOpen Access
    Studies on the effect of different blends on quality of RTS and nectar of aonla (Emblica officinalis Gaertn
    (CCSHAU, 2004) Satish Kumar; Godara, R.K.
    The investigation on the effect of different blends on quality of RTS and nectar of aonla were carried out during the year 2003-04 at Fruit Technology Laboratory, Department of Horticulture, CCS Haryana Agricultural University, Hisar. The present study were conducted to standardize the appropriate blending for different RTS and nectar of aonla. The preserved ready-to-serve and nectar were evaluated for different chemical changes at room temperature for three months. Best quality RTS beverage can be prepared by keeping 10% pulp, 15% TSS, 0.30% acidity with aonla (50%) + guava (50%) ratio. Amal (50%) + guava (50%) RTS beverage scored the highest points as compared to other treatments. In aonla-guava blended nectar, aonla (50%) + guava (50%) nectar was found best. It has excellent quality till 90 days of storage. In aonla-pineapple blended nectar, aonla (50%) + pineapple (50%) nectar was found best. It will scored the highest points as compared to other treatments. The pulp content was 20%, TSS 15% and acidity was 0.3% in both the nectars.
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    ThesisItemOpen Access
    Biomass Production and Nutrient Contents in 8-year old Poplar Plantation
    (CCSHAU, 2006) Satish Kumar; Ravi Kumar
    In order to study the “Biomass Production and Nutrient Contents in 8-year old Poplar Plantation” 8 years old popular plantations grown under 5x4 m, 10x2.5 m and 15x2.5 m spacing were selected. Seven trees in each spacing were felled for recording observations for biomass production and nutrient analysis. The total amount of biomass production was maximum in 5x4 m spacing, followed by 10x2.5 spacing and minimum in 15x2.5 m spacing. About 75 percent of biomass was contributed by stem only. The concentration of N, P and K was maximum in leaves at 15x2.5 m spacing and decreased with decrease in spacing. Secondary nutrients (Ca, Mg, S) also followed the similar trend. Their concentration was also maximum in leaves. Concentration of Zn, Fe, Cu and Mn was recorded maximum in 1.5x2.5 m spacing and decreased with increase in spacing. Zn and Cu concentration were maximum in leaves, while the concentration of Fe & Mn were higher in roots. The uptake of all nutrients analysed was maximum at 5 x4 m spacing and their major portion was accumulated in the stem in all the spacings. In soil concentration of available N, P, K, Ca, Mg, S & micronutrients were maximum at surface layer in all the spacing, which decreased with increase in sampling depth.
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    ThesisItemOpen Access
    Biomass Production and Nutrient Contents in 8-year old Poplar Plantation
    (CCSHAU, 2006) Satish Kumar; Ravi Kumar
    In order to study the “Biomass Production and Nutrient Contents in 8-year old Poplar Plantation” 8 years old popular plantations grown under 5x4 m, 10x2.5 m and 15x2.5 m spacing were selected. Seven trees in each spacing were felled for recording observations for biomass production and nutrient analysis. The total amount of biomass production was maximum in 5x4 m spacing, followed by 10x2.5 spacing and minimum in 15x2.5 m spacing. About 75 percent of biomass was contributed by stem only. The concentration of N, P and K was maximum in leaves at 15x2.5 m spacing and decreased with decrease in spacing. Secondary nutrients (Ca, Mg, S) also followed the similar trend. Their concentration was also maximum in leaves. Concentration of Zn, Fe, Cu and Mn was recorded maximum in 1.5x2.5 m spacing and decreased with increase in spacing. Zn and Cu concentration were maximum in leaves, while the concentration of Fe & Mn were higher in roots. The uptake of all nutrients analysed was maximum at 5 x4 m spacing and their major portion was accumulated in the stem in all the spacings. In soil concentration of available N, P, K, Ca, Mg, S & micronutrients were maximum at surface layer in all the spacing, which decreased with increase in sampling depth.