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20173
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Author: Pooja × End date: 2017 × Start date: 2017 × Type: Thesis ×
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    ThesisItemOpen Access
    Studies on the effect of plant growth regulators and nutrients in Kinnow mandarin
    (CCSHAU, 2017) Pooja; Ashwani Kumar
    The experiment comprising of thirteen fertilizer treatments in different combinations of growth regulators and nutrients was conducted on seven years old earmarked plants at Chaudhary Farm House, Malapur and in Post-harvest Technology Laboratory of the Department of Horticulture, CCS Haryana Agricultural University, Hisar during the year 2015-16 to study the effect of on fruit drop, yield and quality of Kinnow mandarin. The data were recorded on June drop, pre-harvest drop, number of fruits per plant, fruit weight, fruit yield, percent fruit retention, fruit length, fruit diameter, shape index, peel thickness, peel thickness index, juice content, rag content, peel content, TSS, acidity, ascorbic acid, total sugars, reducing sugars, non-reducing sugar and leaf N, P, K and Zn content. The recorded data were analysed using Randomized Block Design. The results of the experiment indicated that the foliar application of 2, 4-D, GA3, K2SO4 and ZnSO4 had significantly positive influence on most of the recorded parameters. The final fruit retention, number of fruits per plant, fruit yield, ascorbic acid content, shape index and non-reducing sugars were found maximum while June and pre-harvest drop was found minimum with foliar application of 2,4-D 20 ppm. The juice content and TSS were found maximum while peel thickness, acidity, peel content were found minimum with foliar application of 2,4-D 10 ppm. The total sugars, fruit size and fruit weight were observed maximum with K2SO4 2% while rag content was found maximum with GA3 20 ppm. The reducing sugar was found highest with foliar application of K2SO4 1.5%. The content of potassium and zinc content was found maximum with the foliar application of K2SO4 2% and ZnSO4 0.75% where as nitrogen and phosphorous content were found unaffected by the application of 2,4-D, GA3, K2SO4 and ZnSO4.
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    ThesisItemOpen Access
    Physiological studies in wheat genotypes (Triticum aestivum L.) under terminal high temperature conditions
    (CCSHAU, 2017) Pooja; Munjal, Renu
    The present investigation was conducted to evaluate various physiological traits & identify promising wheat genotypes under terminal high temperature condition. The twenty genotypes i.e. ten tolerant (WH 730, WH 1124, WH 1021, HD 3059, DBW 90, PBW 373, Raj 3765, HD 2851, HD 2285, PBW 550) & ten heat susceptible (HD 2967, DPW 621, WH 1105, DBW 88, HD 3086, HD 2733, WH 711, WH 1080, WH 1142 & K 0307) were grown during rabi season of 2016 - 17 at Field Crop Research Area, of Wheat & Barley Section, Department of Genetics & Plant Breeding, CCS HAU, Hisar, The experiment was laid out in factorial RBD consisting of 4 rows of 3m length with a 20×5 cm spacing within rows & between plants, respectively. Terminal high temperature significantly influenced physiological, biochemical, yield & yield attributes in both heat susceptible & heat tolerant genotypes but the reduction was more pronounced in the susceptible genotypes in comparison to the tolerant ones. WH 1021 was adjudged to be most tolerant genotype due to minimum reduction in photosynthetic rate, transpiration rate, chlorophyll content & minimum increase in canopy temperature, cell membrane stability. WH 1021 also showed maximum no. of productive tiller/ m row length, biomass, grain yield, thousand grain weight & maximum harvest index. K 0307 was adjudged to be most susceptible due to maximum reduction in all physiological & biochemical trait except chlorophyll fluorescence & canopy temperature. K 0307 showed poor performance in yield & yield attributes. The tolerant genotype WH 1021 was found to be the most suitable one under terminal high temperature conditions on the basis of the overall picture of physiological, morpho-physiological & yield traits studied.
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    ThesisItemOpen Access
    Isolation and characterization of imidacloprid degrading bacteria
    (CCSHAU, 2017) Pooja; Goyal, Sneh
    Imidacloprid, a chloronicotinyl insecticide is used in various crops like, cotton, rice, cereal, maize, sunflowers, potatoes and vegetables for the control of biting and sucking insects, including rice hoppers, aphids, thrips, whiteflies, termites, turf insects. So, there is strong need to remediate imidacloprid. The best method used is bioremediation using microorganisms mainly because of its eco-friendliness and cost effectiveness. During the present investigation, thirty one mutually morphologically distinct bacterial isolates were obtained from imidacloprid contaminated soil samples from Jalalpur kalan village and HAU fields by enrichment culture technique. Eight bacterial isolates showed growth up to 30,000 ppm imidacloprid amended in Mineral salt medium (MSM) containing glucose (0.2%). Out of eight, four isolates showing good growth onMSM agar plates containing 30,000 ppm of imidacloprid were selected for different carbon and nitrogen source utilization pattern on MSM agar plates. All the isolates showed good growth in presence of five different carbon and nitrogen sources. Therefore, these carbon and nitrogen sources were selected for imidacloprid utilization in MSM.More bacterial count and protein content was observed in the medium amended with glucose as carbon source and ammonium chloride as nitrogen source as compared to medium amended with other carbon and nitrogen sources with all the four isolates. To study the utilization of imidacloprid in liquid medium (containing glucose and ammonium chloride), medium was amended with 100 ppm imidacloprid. After 7 days of growth, residual imidacloprid was determined in the medium. Maximum utilization of imidacloprid was found with the isolate IP5 (83%) followed by IP1 (82.2 %), IP6 (74.5%) and IP7 (55.1%) respectively. Growth of four selected isolates was studied in sterilized as well as unsterilized soil amended with imidacloprid (100 ppm) for a period of two months under laboratory conditions. Viable count was higher in imidacloprid amended soil as compared to unamended soil with all the isolates. Imidacloprid level decreased in all the treatments. Percent degradation of imidacloprid was found more in unsterilized soil as compared to sterilized soil. Maximum utilization of imidacloprid was found with the isolate IP5 (76.0 %) followed by IP1 (73.0 %), IP6 (72.0%) and IP7 (57%) in sterilized soil. Similar trend was observed in unsterilized soil i.e. maximum utilization of imidacloprid with the isolate IP5 (82.2 %) followed by IP1 (80.9 %), IP6 (77.27%) and IP7 (68.34 %) respectively. A pot experiment was conducted to evaluate the potential of the two imidacloprid utilizing bacterial isolates IP1 and IP5 in the presence of cotton plants under natural conditions. Germination of seeds was not observed in the treatments amended with 200 ppm imidacloprid. Shoot and root growth was found to be significantly higher in the inoculated treatments amended with 50-100 ppm imidacloprid as compared to their respective uninoculated treatments. Significant decrease in imidacloprid content was observed in soil on inoculation with bacterial isolates. Promising isolates were identified on the basis of 16 s rDNA gene sequencing. Bacterial isolate IP1 was found to be most similar to Bacillus pumilus strain NFB1 and IP5 was found to be most similar to Bacillus aryabhattai.