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2006 - 200912010 - 20181
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Author: Parveen × End date: 2018 × Type: Thesis ×
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    ThesisItemOpen Access
    Energetics and techno-economic feasibility of paddy straw management technologies
    (CCSHAU, 2018) Parveen; Jain, Mukesh
    Paddy straw management using different combinations of machinery i.e. T1 = Combine harvester with SMS (CSMS) + Zero till drill, T2 = CSMS + Spatial till drill, T3 = CSMS + Happy seeder, T4 = CSMS + Reversible mould board plough + Rotavator + Seed drill, T5 = CSMS + Rotavator (2 pass) + Seed drill (SD), T6 = CSMS + Disc harrow (3 pass) + Planker + SD, T7 = CSMS + Rotavator + Manual broadcasting + Rotavator, T8 = Traditional combine (TC) + Stubble shaver + Straw baler + Disc harrow (2 pass) + Planker + SD, T9 = TC + Stubble shaver + Hay Rake + Straw baler + Disc harrow (2 pass) + Planker + SD, T10 = TC + Traditional straw removing method + Disc harrow (2 pass) + Planker + SD with the objective of quantifying the energy requirement and to evaluate techno-economic feasibility. From the experiments, it is concluded that the specific energy consumption and unit cost of operation were in the range of 25 to 28 MJ q-1 and 63 to 68 Rs. q-1 for treatment T1, T2 and T3 respectively. Under the treatment T10, the specific energy consumption and unit cost of operation were 38 MJ q-1 and 74 MJ q-1, respectively. Under the treatment T4, T6, T7, and T8 the specific energy consumption was in the range of 50 MJ q-1 and unit cost of operation was in the range of 112-135 Rs. q-1. Highest specific energy consumption (58.15 MJ q-1) in treatment T5 and highest unit cost of operation (140.52 Rs. q-1) was found in treatment T9.
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    ThesisItemOpen Access
    Agrobacterium mediated transformation of indica rice variety HKR46 with amaranthus seed albumin gene, AmA1
    (CCSHAU, 2006) Parveen; Kharb, Pushpa
    Experiments were conducted to transfer the Amarantus seed albumin gene, AmA1, driven by CaMV35S promoter in indica rice variety HKR46 by Agrobacterium-mediated transformation. The japonica rice variety TNG67 was also used as control for transformation. During the present investigation, 15-18 days old calli initiated from mature seed scutella and immature embryos on modified MS medium were used. The calli were co-cultivated for 10 minutes in liquid co-cultivation medium with Agrobacterium strain EHA105 (pSB8) and then transferred onto the solidified co-cultivation medium for two days. After two days the co-cultivated calli were transferred onto selection medium containing kanamycin/paromomycin (50 mg/l) for 2-3 cycles of selection of 15 days each. A total of 13/1717 and 103/278 calli of HKR46 and TNG67 respectively showed sustained proliferation in the selection medium. The putatively transformed calli were then transferred onto regeneration medium. No shoot regeneration was obtained in case of indica rice variety HKR46. However, eight of the putatively transformed calli of TNG67 regenerated shoots; but all of these shoots were albino. The study shows an urgent need of developing an efficient protocol for high frequency green plant regeneration/genetic transformation in indica rice varieties.