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2020 - 20233
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Author: Nisha × End date: 2023 × Start date: 2020 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    An economic analysis of production and processing of groundnut in Haryana
    (CCSHAU, Hisar, 2023-10) Nisha; Pawar, Neeraj
    The present study was carried out with the objectives of working out costs and returns of groundnut cultivation, to study processing costs and returns of groundnut and to identify the constraints in production and processing of groundnut. The study was based on collection of primary data. The primary data from selected farmers for the year 2021-22 were collected by survey method through personal interview with the help of a well-structured interview schedule. For collection of data multistage purposive sampling technique was used. The selection of districts formed the first stage of sampling. Out of major groundnut growing districts of Haryana, Fatehabad, Hisar, Sirsa and Rewari districts were selected purposively based on the area under groundnut for the present study. Selection of farmers and processing units formed the ultimate unit in the sample. From seven selected villages from four selected districts, total 84 farmers were selected, comprising 21 farmers from each district. A total of 7 processing units were selected from the study area as per availability. The outcomes of the study revealed that the average total cost of cultivation of groundnut in four districts came out to be Rs. 98275. The total cost incurred by the farmers of Rewari district was higher (Rs. 106886/ha) compared to that of Sirsa (Rs. 100271/ha), Fatehabad (Rs. 93789/ha) and Hisar (Rs. 92155/ha). The average yield of the groundnut was 24.61 q/ha which was highest in Rewari district being 29.15 q/ha as compared to Sirsa (25.17 q/ha), Fatehabad (22.30 q/ha) and Hisar (21.83 q/ha).The gross return obtained after inclusion of value of by product, per hectare was again highest in Rewari district (Rs. 169376) as compared to Sirsa (Rs. 145692), Fatehabad (Rs. 129242) and Hisar (Rs. 127695). The average net return of the groundnut for selected respondents as a whole was Rs. 44997 per hectare. As B: C ratios are greater than one, this indicates that groundnut cultivation is economically viable in all the districts, with Rewari district having the highest profitability. Total cost per quintal of groundnut processing was higher in oil processing units (Rs.17289.33/q) when compared to decorticating units (Rs.8273.57/q). In respect of net returns, it was also higher in case of oil processing units (Rs. 161.55/q) compared to decorticating units (Rs. 130.59/q). On an average a minimum quantity of 342 quintals of groundnut kernel and 813 quintals of groundnut oil should be processed so as to continue the production process without sustaining losses at average level in decorticating units and oil processing units respectively. The identified constraints, such as less availability of improved varieties/ seeds and high cost of seeds, high incidence of pest and disease infection and problem of uncertain weather/ climate, pose significant challenges for groundnut farmers, impacting their productivity and profitability. The scarcity and high wages of skilled labour during peak seasons, high machinery costs, erratic power supply, repair and spare parts problems, complex bank finance procedures, expensive raw materials, and high transportation costs were all identified as significant challenges.
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    ThesisItemOpen Access
    Computational analysis of potential phytochemicals interaction with COVID-19 main protease
    (CCSHAU Hisar, 2022-07) Nisha; Ahalawat, Navjeet
    The global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 2020 has affected almost every country with around 543 million confirmed cases worldwide. Currently to fight against the infection, multiple vaccines and several types of drugs alone or with combination have been used in many countries for emergency use. Some severe side effects of repositioned drugs are a cause for concern and their efficacy is still under evaluation. Antiviral therapeutics based on phytochemicals that have been reported to have more tolerable side effects can serve as a reliable alternative to synthetic antiviral drugs for the inhibition of viral replication and penetration. The main protease of SARS-CoV-2 is one of the highly potent and vital targets to design and develop antiviral drugs for the inhibition of COVID-19 contamination. Computational approach helps to identified many drugs that can target viral proteins Mpro. We generated a phytochemical library containing 2453 phytochemicals which have been reported as having antiviral activity through deep literature study.The virtual molecular docking results reveals that five bioactive compounds; theaflavate C, theaflavin-3,3-O-digallate, amentoflavone, hinokiflavone, theaflavin3-gallate have a higher binding affinity toward COVID-19 main protease (MPro) with the binding energy of -10.1, -9.9, -9.7, -9.7 and -9.7 Kcal/mol. But these phytochemicals didn’t follow Lipinski rule of five, so we selected more four phytochemicals having ADMET properties. The main protease docked complexes with phytochemicals; theaflavate C, theaflavin-3,3-O-digallate, amentoflavone, hinokiflavone, theaflavin3-gallate, 10-methoxycamptothecin, caribine, sesamin and licoisoflavanone were used for MD simulation of 20 ns time period using Gromacs package. The MD results were analysed with respect to RMSD, RMSF, Rg, SASA, hydrogen bond and principal component analysis. The resulting trajectories of converged period of MD were further exploited in MM-P/G/BSA calculations to derive accurate estimates of binding free energies. The study provides a basic foundation and suggests that the seven phytochemicals, viz. theaflavate C, theaflavin-3,3-O-digallate, amentoflavone, hinokiflavone, theaflavin3-gallate, caribine, and licoisoflavanone serve as potential inhibitors in regulating the Mpro protein’s function and controlling viral replication and may assist the development of effective anti-COVID-19 drugs.
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    ThesisItemOpen Access
    Biochemical studies on fatty acid esterase isolated from pearl millet and wheat flours
    (CCSHAU, Hisar, 2020) Nisha; Chugh, L. K
    The present investigation was conducted on pearl millet cv. HC 20 and wheat cv. WH 1105. Pearl millet fatty acid esterase (PM-FAE) and wheat fatty acid esterase (WH-FAE) were partially purified from by ammonium sulphate fractionation (30-60 % saturation) followed by dialysis by 3.03 and 7.75 folds with specific activity of 15.35 and 22.5 units/mg protein and recovery of 57and 56 % of the activity, respectively. PM-FAE and WH-FAE, both exhibited optimum pH of 8.0. Optimum temperature for PM-FAE (40oC) was lower than WH-FAE (45oC). WH-FAE was comparatively more stable at high temperature (70OC). Km value of PM-FAE (0.129 μM p-NPB) was lower than that of WH-FAE (0.28 μM p-NPB). Km values of PM-FAE for p-NPB and NPO were about 50 % lower compared to that of WH-FAE. Affinity of PM-FAE and WH-FAE for NPD was approximately equal. Ascorbic acid and palmitoyl ascorbate inhibited both FAEs in a concentration dependent manner. After 5 days of storage, reduction of 73 % in total buildup of fat acidity (FA), 84% reduction in rate of increase in FA, 55 % inhibition of in vitro activity of FAE, 52 % inhibition in situ activity of FAE and 50 % inhibition of activity of lipoxygenase (LOX) in 0.58 % ascorbic acid-fortified flour compared to unfortified flour were observed. Inhibitory effect of ascorbic acid fortification on in vitro activity of FAE, in situ activity of FAE and LOX increased with fortified concentration of ascorbic acid whereas rate of increase in FA was almost unaffected. Inhibition of LOX (62-69 %) in 0.84 % ascorbic acid-fortified flour was comparable to that (68-74 %) of blanched grain flour (BGF) throughout storage period. It was concluded that comparatively higher activity of PM-FAE in flour, lower Km value for its substrates and lower optimum temperature might be responsible for faster in situ hydrolysis of lipids and hence poor shelf life of pearl millet flour than wheat flour. Indirect fortification of flour by ascorbic acid treatment of pearl millet grains was effective in negatively modulating activities of FAE and LOX in flour for arresting not only in situ hydrolysis but also enzymatic oxidation of lipids.