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2000 - 200922010 - 20183
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Author: Monika × Start date: 1996 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Evaluation of management practices against Polyphagotarsonemus latus (Banks) and its role as vector of leaf curl virus in chilli
    (CCSHAU, 2018) Monika; Gulati, Rachna
    Under in vitro 35.00 to 67.27 and 33.89 to 63.89 percent mortality of P. latus was recorded in Direct Spray Bioassay, with 2.5 to 10.0 % and 1.25 to 10.0 % seed and leaf extract concentrations of P. pinnata, respectively. LC50 values of methanolic seed and leaf extract of P. pinnata were 5.36 and 5.31, respectively for mobile stages of P. latus. Under field conditions, the percent reduction in P. latus was from 40.60 to 72.55 percent with all the treatments concentration of P. pinnata. Under in vivo P. pinnata seed extract 10.0% (72.55%), was the most potent in reducing the mite population followed by P. pinnata leaf extract 10.0% (65.10 %), P. pinnata seed extract 7.5 % (62.66 %), P. pinnata seed extract 5.4 % (55.49%), P. pinnata leaf extract 7.5 % (54.98 %), P. pinnata seed extract 5.0 % (48.33%), P. pinnata leaf extract 5.3 % (47.58%), P. pinnata leaf extract 5.0 % (40.16%) and Pyriproxyfen 5% + Fenpropathrin 15% EC (1ml/l H2O) (37. 83%), respectively. Studies on seasonal incidence of P. latus revealed a peak in its population on chilli during second fortnight of October during the respective crop seasons in both the years under field conditions. Strata wise, top strata harboured significantly more number of mites followed by middle and bottom strata. A comparison of mulch treatments showed that Organic mulch 12t/ha was most effective in reduction of P. latus population (79.30 %), followed by Black/Silver polythene sheet (78.50 %), Black/White polythene sheet (78.30 %), Black polythene sheet (77.50 %), Organic mulch 9t/ha (74.70 %) and Organic mulch 6t/ha (65.30 %) treatment as compared to control. In terms of percent increase in yield, Organic mulch 12t/ha caused highest increase (57.90 % over control) followed by Black polythene sheet mulch, Black/Silver polythene sheet mulch, Black/White polythene sheet mulch, Organic mulch 9t/ha and Organic mulch 6t/ha showing 47.49, 46.72, 45.17, 42.47 and 34.36 percent increase over control. P. latus showed non-transmissible nature of virus. Thus it indicated that mite is not a carrier vector for transmission of leaf curl virus into chilli plant.
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    ThesisItemOpen Access
    Improvement in the symbiotic efficiency of mungbean rhizobia through application of micronutrients
    (CCSHAU, 2017) Monika; Leela Wati
    Micronutrients play an important role in increasing yield of pulses and oilseed legumes through their effects on the plant itself and on the nitrogen fixing symbiotic process. Indian soils have become deficient not only in major plant nutrients like nitrogen, phosphorous, potash but also scarce in some micronutrients like zinc, iron, molybdenum and boron. These situations suggest the need of application of micronutrients with biofertilizer under intensive cultivation of legumes as it is directly involved in biological nitrogen fixation through nitrogenase enzyme activity In the present study, a total of sixty one rhizobial isolates were retrieved from healthy mungbean root nodules using Yeast extract mannitol agar medium (YEMA). Three rhizobial isolates obtained from departmental culture collection were also included in this study. After isolation, all the rhizobial isolates were assessed for temperature tolerance (up to 450C) and salt (NaCl) tolerance (up to 5%) and characterized for plant growth promoting traits: Indole acetic acid production, phosphate solubilization, siderophore production, HCN production and ACC utilization. IAA production varied in the range of 1.19- 59.19 μg/ml and the isolate MRH59 produced maximum IAA (59.19 μg/ml) after 5 days incubation at 30 0C.The ability of rhizobial isolates to excrete ammonia, varied from 0.61 to 4.67 μg/ml. Out of sixty four rhizobial isolates, 25% rhizobial isolates showed siderophore production, 15% rhizobial isolates showed HCN production and overall 55% of rhizobial isolates showed significant ACC utilization on minimal medium plates. P-solubilization index (P-SI) varied from 0.5-2.9 after four days of incubation at 30ºC. On the basis of plant growth promoting traits, isolate MRH 59 was selected to study the effect of different concentration of micronutrients (Fe, Mo, B and Zn) on growth of mungbean rhizobia. Among various micronutrients, combination of micronutrients and different concentrations of micronutrients; 0.5% ferrous sulphate+ ammonium molybdate application in YEM broth showed maximum (11.225 log no of cfu/ml), followed by 1% ferrous sulphate (10.968 log no of cfu/ml) and 1% ammonium molybdate (10.481 log no. of cfu/ml). Ten promising rhizobial isolates namely MRH1, MRH2, MRH3, MRH4, MRH16, MRH46, MRH46, MRH48, MRH59 and MB703 which showed plant growth promoting traits were selected for plant growth evaluation of mungbean under chillum jar conditions. Among ten rhizobial isolates, mungbean plants inoculated with isolate MRH59 showed maximum nodule no, nodule weight root length, shoot length, plant dry weight, pod no, seed weight, and seed no under chillum jar conditions. Isolate MRH 59 and MB703 (as a reference strain) along with different micronutrient concentrations (1% ferrous sulphate, 1% ammonium molybdate and 0.5% ferrous sulphate+ammonium molybdate) were selected for plant growth promotion of mungbean under pot house conditions. Among various treatments, 1% ammonium molybdate application along with inoculation of rhizobial isolate MRH59 and PSB showed maximum nodule no, nodule weight, plant dry weight, seed yield, N-uptake and P-uptake. On the basis of 16S rDNA sequencing the promising isolate MRH59 was found to be much similar to Sinorhizobium sp. T25.
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    ThesisItemOpen Access
    Empowering Rural Women Through Self Help Groups (Shg S) In Haryana - A Study Of Swa Shakti Project
    (Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2007) Monika; Chanderbhan
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    ThesisItemOpen Access
    Impact Of Nutrition Education And Supplementation On Nutritional Status, Knowledge And Practices Of Adolescent Girls
    (Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2000) Monika; Sehgal, Salil
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    ThesisItemOpen Access
    Mapping QTL(s) for drought tolerance in Indian mustard (Brassica juncea L. Czern & Coss.)
    (CCSHAU, 2015) Monika; Yadav, R. C.
    The present investigation on studies on Mapping QTL(s) for drought tolerance in Indian mustard (Brassica juncea L. Czern & Coss.) was conducted with the objective to identify QTL(s) for drought tolerance in RB50 x Kranti derived F 2 and F2:3 populations of Brassica juncea (RB50-Drought tolerant, Kranti-Drought sensitive)and to evaluate both generations for various phenological traits, growth related traits, physiological traits along with yield and its component traits under irrigated and drought conditions. RB50 × Kranti derived F 2 and F2:3 population showed significant variation for various observed agronomic traits. 200 SSR primers from various Brassica species were used to screen parental genotypes (RB50, Kranti) and 51 markers were found polymorphic. These polymorphic SSR primers were used to screen F2 population and were used to construct linkage map of Brassica juncea using MapmakerExp3.0 covering 10 linkage groups, 2 SSR markers did not show linkage with any of the marker groups and hence did not map to any of the linkage group. Composite interval mapping (CIM) analysis revealed a total of 30 QTLs in F 2 generation under irrigated conditions for various drought related, yield and other traits in Brassica juncea out of which four QTLs were identified for drought related physiological trait (Electrolyte leakage). A total of 19 QTLs were identified in F 2:3 under irrigated conditions for various drought related, yield and other traits in Brassica juncea including 4 QTLs for physiological trait (Relative water content). QTL analysis revealed a total of 7 QTLs accounting for different phenotypic variance in F 2:3 generation under drought conditions for various drought related, yield and other traits in Brassica juncea with one QTL identified for drought related physiological trait (Electrolyte leakage). There was clustering of QTLs on many LGs in linkage map. Most prominent clustering signifying multifunctional QTL region was observed in the LG 5 and 10. This multifunctional QTL region on the LG 5 contains at least one major QTL for various traits. Linkage groups 5 and 10 have been suggested to have major QTLs affecting drought tolerance. QTLs identified in the present study firstly needs to be validated in other populations and then fine mapping of these drought related QTLs have to be carried out them in marker assisted selection and breeding for drought tolerant genotypes in Brassica juncea.