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2003 - 200912010 - 20183
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Author: Manisha × End date: 2019 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Nutritional evaluation of products prepared from wheat and dried water chestnut composite flour
    (CCSHAU, 2018) Manisha; Punia, Darshan
    The present investigation was carried out to study the nutrient composition of wheat and dried water chestnut composite flour, development of products using composite flour and their sensory and nutritional evaluation and studying the shelf life of the developed products. Four types of composite flours were prepared with using wheat and water chestnut in ratios; 80:20, 60:40, 40:60 and 20:80. Six products namely, chapati, halwa, ladoo, nankhatai, biscuits and cake were prepared using all types of composite flour in which chapati, halwa, nankhatai and biscuits developed from type I composite flour were most acceptable and ladoo prepared from type III composite flour and cake from type II composite flour were most acceptable. These acceptable products were evaluated in terms of sensory and nutritional parameters. The results of proximate composition revealed that water chestnut flour contained 9.36 % moisture, 8.03% crude protein, 2.33% crude fat and 76.55 % total carbohydrates. Calcium content of water chestnut flour was 34.29 mg/100g. Total phenols, flavonoids and antioxidant activity (DPPH) of water chestnut flour were 7.13 mg GAE/100g, 1.63 mg RE/100g and 2.62 mg TE/100g, respectively. The value added products were evaluated organoleptically using nine point Hedonic scale by a panel of ten judges. Mean scores of organoleptic evaluation indicated that all the products were acceptable in terms of their color, appearance, aroma, texture and taste. Overall acceptability scores of the all products revealed that all products were in the category of “liked slightly” to “liked very much” by the judges. Sensory evaluation of products showed that water chestnut flour could be incorporated up to 60% level in ladoo, 40% level in cake and 20% level in chapati, halwa, nankhatai and biscuits. Further nutritional evaluation of products revealed that with the incorporation of water chestnut flour in wheat flour, the content of total sugars, starch, total carbohydrates, calcium, total phenols, total flavonoids and antioxidant activity (DPPH) were increased significantly whereas crude protein,crude fat, crude fibre, iron, zinc, potassium and magnesium content were found to be decreased significantly. Shelf life study of most acceptable and storable products (nankhatai, ladoo and biscuits) was done. There were no significant differences were observed in the overall acceptability of the storage of products. It could be concluded that water chestnut flour can be incorporated in development of products.
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    ThesisItemOpen Access
    Assessment of Nutritional Status of Preschool Children of Jind District (Haryana)
    (I.C College Of Home Science Chaudhary Charan Singh Haryana Agricultural University Hisar, 2003) Manisha; Punia, Darshan
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    ThesisItemOpen Access
    Computational modeling and molecular dynamic simulation of pyrophosphatase of rice (Oryza sativa L.)
    (CCSHAU, 2018) Manisha; Sudhir Kumar
    Inorganic pyrophosphatase plays a significant role in various processes in plants. It causes chalkiness and hydrolytic breakdown of ADP-glucose in plastidal compartment. It has significance in lipid metabolism, calcium absorption, DNA synthesis and biochemical transformations. The sequence of inorganic pyrophosphatse was retrieved from NCBI and template was identified using BLASTP. With 84% query coverage and 71% identity 4LUG was selected as template. Modeller 9.19 and RaptorX were used for computational modeling. Predicted models were refined by energy minimization with GROMOS force field from Swiss-pdb Viewer. Minimum energy calculated for Modeller 9.19 and RaptorX predicted models were -2394.489KJ/mol and -7365.312KJ/mol respectively. The structures were assessed by GROMOS, ANOLEA and QMEAN graphs. More favourable region was shown by GROMOS and ANOLEA as compare to QMEAN. WHATIF server programs were used for structures optimization and validation. Bond length Z-score, bond angle Z-score, coarse packing quality and Ramachandran Z-score, were approximately 0.4, 1.2, -0.9 and 0.1 respectively. SAVES server programs score for PROVE, VERIFY3D and ERRAT were approximately 4.2%, 81% and 91% respectively. Ramachandran plot calculated by PROCHECK showed approximately 94% amino acid in core and 6% in allowed region. The models visualization showed coils were dominantly present in both the structures. RMSD for the structures was less than 0.5. Explicit solvent molecular dynamic simulation was done by VMD and NAMD software. The total energy and RMSD graphs calculated after simulation were stable for the structures. Structure superimposition with template showed significant conserved region between template and predicted structures. RMSD calculated after simulation was less than 0.5 Å against for both models template. The model predicted by RaptorX was found better as compared to Modeller 9.19 predicted model.
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    ThesisItemOpen Access
    Biology of polyommatus boetius (L.) on fieldpea and evaluation of various genotype against major insect pests
    (CCSHAU, 2016) Manisha; Roshan Lal
    The present studies entitled “Biology of Polyommatus boeticus (L.) on fieldpea and evaluation of various genotypes against major insect pest” was carried out during rabi 2015-16 at Reseach farm of Pulses Section, Department of Genetics and Plant Breeding and Laboratory of Department of Entomology, CCS HAU, Hisar. For conducting biological studies, rearing was done in laboratory at 28±10C and relative humidity (60-70%). Results revealed that pre-oviposition, oviposition and post-oviposition period ranged from 1 to 2, 2 to 3 and 2 to 3 days, respectively. A single female could lay maximum of 15 eggs and minimum of 13 eggs (average 14 eggs) during her life time. On fieldpea, the longevity of male moths was ranged from 7 to 9 days (average 8 days) and longevity of female moths varied from 9 to 10 days (average 9.5±0.42 days). Incubation period varied from 2 to 3 days (average 2.5±0.7 days). The total larval period ranged from 11 to 13 days (average 12±0.22 days). Average Prepupal period ranged from 2 to 3 days (average 2.22±0.13 days) and pupal period ranged from 6 to 9 days (average 8±0.22 days). Average female to male sex ratio was 1.0 to 1.5. It was found that the mating of adults of P. boeticus occurred in day time only. When the mating was disturbed the pair flew without separation and continued mating. Mating period of P. boeticus varied from 48 to 64 minutes (average 54.3 ±0.07 minutes). Results revealed that the total life cycle varied from 29 to 35 days (average 32.8±4.24 days). Morphometric studies on P. boeticus revealed that the eggs measured 0.47 to 0.57 mm in diameter (average 0.52 mm). Average body lengths of 1st, 2nd, 3rd and 4th instar larvae were 1.17, 2.10, 7.59 and 11.09 mm and average body width of 1st, 2nd, 3rd and 4th instar larvae were 0.16, 0.69, 1.15 and 1.39 mm. Average head capsule length of 1st, 2nd, 3rd and 4th instar larvae were 0.22, 0.70, 1.25 and 1.46 mm. Average head capsule width of 1st, 2nd, 3rd and 4th instar larvae were 0.15, 0.64, 1.10 and 1.34 mm. On an average, pre pupa measured 10.80 mm in length and measured 4.94 mm in width and pupa measured 10.62 mm in length and measured 4.45 mm in width. On an average 1st, 2nd, 3rd, 4th larval instar, pre pupa and pupa male weighted 0.004, 0.021, 0.064, 0.170, 0.155 and 0.088 mg, respectively. Immature stage was not distinguishable in male and female. Adult antennae, fore leg, middle leg and hind leg measured 7.93, 5.78, 6.05, 6.28 mm long in female and 7.02, 5.63, 5.91 and 6.15 mm in male. The average wingspan measured 29.90 mm in male and 33.28 mm in female. Out of the 18 genotypes screened against pod borer complex comprising H. armigera, E. zinckenella and P.boeticus at green pod stage, the infestation ranged from 0.11 to 1.22, 0.00 to 1.44 and 00 to 0.33 larvae/ 3 plants, respectively, whereas at maturity it varied from 0.48 to 7.53, 7.31 to 22.10 and 0.50 to 1.60 per cent, respectively. Genotypes HFP-1137, HFP-530B, and HFP-529 were least susceptible and genotype HFP-8712 was highly susceptible against H. armigera. There was positive and significant correlation of 50 per cent flowering with H. armigera and E. zinckenella. Pod wall thickness had negative and significant correlation with H. armigera and E. zinckenella. Pod length was positive and significantly correlated with P. boeticus pod damage. Trichome density was negative and significantly correlated with E. zinckenella larval population and P. boeticus pod damage. Seasonal incidence studies revealed that C. indicus population had significant negative correlation with temp (min.). H. armigera and P. boeticus population had non- significant correlation with abiotic factors. E. zinckenella larval population had significant positive correlation with Temperature (Tmax.). C. horticola population had significant negative correlation with temp.(max & min) and significant positive correlation with morning relative humidity.