Thumbnail Image

Theses

Browse

2019 - 201912020 - 20231
Reset filters
Author: Kamboj, Aarti × End date: 2023 × Type: Thesis ×
Reset filters

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    ThesisItemOpen Access
    Molecular characterization for targeting foliar blast disease in pearl millet [Pennisetum glaucum (L.) R. Br.]
    (CCSHAU, 2023-04) Kamboj, Aarti; Yashveer, . Shikha
    In the present investigation, 60 pearl millet genotypes were evaluated at experimental field of Bajra Section, CCS HAU, Hisar during kharif season, 2021 for morpho-phenological traits & blast disease and the study of genetic relatedness among them using microsatellite markers. Highly significant mean sum of squares were obtained due to the genotypes for all the traits studied. The traits viz., plant height, ear length, ear diameter, leaf blade width, effective number of tillers per plant, 1000-grain weight, dry fodder yield per plant and grain yield per plant exhibited high heritability (>60%) and high genetic advance as percent of mean (GA%M) (>20%) whereas, the traits ear length, leaf blade width, effective number of tillers per plant, dry fodder yield per plant and grain yield per plant exhibited high Genotypic Coefficient of Variation (GCV) (>20%) and Phenotypic Coefficient of Variation (PCV) (>20%). Correlation coefficient analysis revealed high significant positive correlation coefficients of grain yield with traits viz., plant height, ear diameter and dry fodder yield per plant. As per blast screening, 43 genotypes were found resistant to the disease while 17 genotypes were found susceptible. In order to develop blast resistant hybrids, selected resistant lines were crossed with elite lines of pearl millet and out of the 34 hybrids developed, 32 were found resistant to blast. The increased activities of Phenylalanine Ammonia Lyase (PAL), Peroxidase (POX) and Lipoxygenase (LOX) in the resistant hybrids further confirmed the disease scoring results
  • Thumbnail Image
    ThesisItemOpen Access
    Genetic diversity analysis in Kiwifruit (Actinidia spp.) using RAPD/SSR markers
    (CCSHAU, 2019) Kamboj, Aarti; Kharb, Pushpa
    Kiwifruit (Actinidia spp.) is a very important plantation crop, generally dioecious, deciduous and scrambling vines with chromosome number 2n=58, belonging to family Actinidiaceae. Dioecy in kiwifruit lays an important constraint for breeding programs. The present study was undertaken to study genetic diversity in kiwifruit genotypes using RAPD markers. Molecular polymorphism in 7 kiwifruit genotypes (2 males viz. Tomuri & Allision and 5 females viz. Bruno, Hayward, Monty, Allision & Abbott) was evaluated using 94 RAPD primers, out of which 23 were found to amplify the genomic DNA in all the seven genotypes. Analysis of RAPD data was done using NTSYS-pc software and dendrogram was constructed using UPGMA method. The 7 kiwifruit genotypes grouped in two main clusters comprising of male and female genotypes separately. Similarity matrix indices ranged from 0.521 to 0.719 showing maximum similarity between Allision (M) and Tomuri (M) with a similarity coefficient of 0.719 while Allision (M) and Abbott (F) were found least similar with a similarity coefficient of 0.521. Primers OPA-01, OPB-02, OPB-20 and OPC-05 amplified unique amplicons in Bruno, Hayward, Abbott, Monty and Allision (M), and primers OPA-02 & OPN-02 amplified unique amplicons in Tomuri (M) & Allision (M) and male and female plants of Allision genotype respectively. Thus, these primers can be used to distinguish the kiwifruit genotypes and can also be validated as putative sex linked markers which have the prospective to be used for further characterization in specific kiwifruit genotypes.