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ThesisItem Open Access Study on Menstrual Hygiene Practices among Rural Adolescent Girls in Hisar district(CCSHAU, 2019) Jyoti; Dahiya, ManjuAdolescence in girls has been recognized as a special period in their life cycle that requires specific and special attention. This period is marked with the onset of menarche. Menstruation is a phenomenon unique to all females. This study was undertaken with the aims to assess the knowledge of adolescent‘s girls living in rural area regarding menstruation and also to study their misconception and experience and menstrual hygienic practice regarding menstruation. The present study was conducted in Hisar district of Haryana state. From Hisar district block II was selected randomly and from block II two villages viz. Gawar and Burak were selected randomly. 50 females from each villages were selected randomly. Thus, making a total of 100 respondents. A set of independent variables and dependent variables were selected for the study. In this study majority of the respondents respondents attained the menarche between 13-16 years, in class VIII followed by IX with mothers‘ as a home-maker, elder sibling as a sister, both of them helped the respondents to become more aware about the menarche as both were the major source of information for them. From the findings of the present study, it can be observed that the adolescent girls had proper awareness about menstruation before menarche (75.0%) and duration of menstruation (87.0%). Regarding experience of respondents about menstruation it was found majority knew about so many aspects of menstruation but some misconceptions still exist in the respondents and instead of thinking menstruation as normal process in adolescent girls, they thought (67.0%) it as an impure blood getting released from the body. Majority of the respondents (44.0%) felt upset during their first menstruation and had (60.0%) negative reaction in general. Majority of the respondent faced the problem of (70.0%) stomach pain, (67.0%) backache, (47.0%) irritation and (38.0%) breast soreness before the menstruation, fear (93.0%), lose confidence(88.0%), lazyness (63.0%), and irritation (47.0%) during the menstruation and cent per cent of the respondents felt increased confidence , activeness and good sleep post menstrual feelings. Majority of the respondents were following good menstrual hygienic practices. Use of absorbent was found to be significantly associated with age (χ2 = 16.11*), education (χ2 = 32.625**) and fathers‘ occupation (χ2 = 13.9218*). Age (χ2 = 12.042*), education (χ2 = 20.784*) was significantly associated with reaction about menstruation of the respondents. It was found that age (χ2 = 19.159*), education (χ2 = 21.78*) and caste (χ2 = 29.751**) was significantly associated with awareness about menarche before its onset. Age education of the respondents and education of mother was positively coorelated with menstrual hygienic practices. It can be concluded that the proper menstrual hygiene and correct perception can protect the women from suffering.ThesisItem Open Access Development and nutritional evaluation of micronutrient rich baked and traditional products(CCSHAU, 2018) Jyoti; Jood, SudeshThe present study was conducted to see the effect of supplementation of spinach leaves in commonly consumed baked and traditional products. Five types of composite flours were formulated by using wheat flour, bengal gram flour and spinach leaves powder at (4, 6, 8, 10 and 12% levels). Control-I (100% wheat flour), Control-II (100% bengal gram flour), Type-I composite flour (48:48:4), Type-II composite flour (47:47:6), Type-III composite flour (46:46:8), Type-IV composite flour (45:45:10), and Type-V composite flour (44:44:12), respectively. All the formulated composite flour samples were analyzed for their physico-chemical properties. It was observed that water absorption capacity, oil absorption capacity, swelling power, bulk density and least gelation capacity of control flours increased significantly with increase in the level of incorporation of spinach powder. The values varied from 0.98 to 3.20 ml/g, 1.23 to 2.90 g/g, 7.85 to 9.06 g/g, 0.64 to 0.94 g/ml and 9.00 to 12.40 g/100ml, respectively in all five types of composite flour samples. While solubility and flour dispersability were found to be decreased with increase in the level of spinach powder. Type-I composite flour showed highest values containing 4 per cent level of spinach powder while Type-V composite flour showed lower values containing 12 per cent level of incorporation. Moisture, crude protein, crude fat, crude fibre and ash content of all five types of composite flour samples were found significantly higher than the control sample (wheat flour) while in comparison to bengal gram flour (control), crude protein content in all five types of composite flour samples decreased due to replacement of bengal gram flour with wheat flour. Total iron, calcium, zinc, phosphorus, β-carotene, dietary fibre and anti-oxidant activity of all five types of composite flour found significantly higher than control sample. Seven value added baked (biscuits, buns and kulcha) and traditional (matthi, matar, sev and burfi) products were prepared from formulated composite flour samples. All the products were analyzed for their organoleptic acceptability and nutritional composition. Overall acceptability scores of all four types of composite flour (up to 10% level of spinach leaves powder) made products (biscuits, matthi, matar, sev and burfi) were rated as ‘liked moderately’ to ‘liked slightly’ by the panelists. Whereas buns and kulcha found acceptable up to 8 per cent level of incorporation of spinach leaves powder. All the products were found nutritionally rich in crude protein, dietary fibre, minerals, β-carotene and anti-oxidant activity in comparison to their respective control products. Incorporation of spinach leaves powder in baked and traditional preparations are recommended to enhance the β-carotene, minerals, dietary fibre and anti-oxidant activity of diets for combating micronutrient deficiencies.ThesisItem Open Access Use of molecular markers for varietal identification and assessing phylogenetic status of Basmati rice in genus Oryza sativa L.(CCSHAU, 2011) Jyoti; Jain, SunitaMolecular markers provide novel tools for varietal identification, diversity analysis and assessing phylogenetic relationships among various rice groups in genus Oryza. A set of 50 rice genotypes comprising of seven japonica rice varieties, six traditional Basmati, thirteen cross-bred Basmati and 24 indica rice varieties was investigated using 54 markers (28 on chromosome 8 and 26 on rest of the chromosomes); data obtained has been used to assess the phylogenetic status of Basmati rice in genus Oryza sativa L. A total of 217 alleles were detected, with an average of 4.01 alleles per locus. Number of alleles per locus (3.5 alleles) for markers on chromosome 8 was less than the mean value (4.58 alleles) based on 26 SSR markers on rest of eleven chromosomes of rice. Eleven of these alleles were unique, present in only one genotype. Null allele was observed at only one locus (RM408) in Super Basmati. The number and size of alleles and Polymorphism Information Content (PIC) values ranged between 2-7, 83-381 bp and 0.074-0.810, respectively. An average PIC of 0.55 per locus was obtained, which confirms that markers used in this study were highly informative. Genetic relationships generated using the whole genome marker data as well as allelic profile on chromosome 8 and rest of eleven chromosomes separately placed 50 rice genotypes in three distinct clusters. Seventeen of the nineteen Basmati rice varieties (except two cross-bred Basmati rice varieties, Sabarmati and Improved Sabarmati) formed a separate cluster quite distinct from the indica and japonica rice clusters. Interestingly, mean similarity indices obtained from chromosome 8 dataset placed Basmati rice cluster at equal distance from japonica and indica rice types. However, whole genome as well as rest of the genome marker datasets placed Basmati group closer to indica rice varieties than japonica. Chromosome 8 dataset showed a positive correlation (Mantel test, r = 0.631) with the rest of the genome dataset, indicating a higher level of similarity between the two. All the traditional and crossbred Basmati rice varieties and two aromatic japonica varieties shared the same BAD2 alleles at the aroma locus, indicated the distinctness of the aroma locus. While the present study gives support to the hypothesis that japonica genotypes may have contributed towards the evolution of chromosome 8 or a part of chromosome 8 in Basmati rice, it also indicate the active flow of genes from indica rices during the course of parallel evolution in northern Indian sub-continent. The study also demonstrates the distinctness of TB from other rice types (indica and japonica) and also provides a number of novel SSR markers which can be used to differentiate within/among the various rice groups at commercial level. It was observed that the amplified products differing in ≥12bp can be separated on 3% agarose gels for varietal identification in Basmati rice, which is faster, much cheaper and relatively simple method compared to the advanced molecular methods already available for the purpose.ThesisItem Open Access Use Of Molecular Markers For Warietal Identification And Assessing Phylogenetic Status Of Basmati Rice In Genus Oryza Sativa L.(Department Of Biochemistry College Of Basic Sciences And Humanities CCS Haryana Agricultural University : Hisar, 2010) Jyoti; Jain,Sunita.