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ThesisItem Open Access Dissecting the role of OsRuvB transgene for salt tolerance in transgenic chickpea (Cicer arietinum L.)(CCSHAU, Hisar, 2023-03-15) Chaudhary, Rinku; Kharb, PushpaChickpea (Cicer arietinum L.), a self-pollinated diploid (2n = 2x = 16), is one of the most important grain legume with a genome size of approximately 740 Mbp. Chickpea is an annual grain crops with global production of 18.7 million tons, including 11.38 million tons in India. Chickpea is highly sensitive to salinity and its growth is highly affected. Soil salinity decreases the productivity of legume crops including chickpea, which is known for its sensitivity towards salinity. About 20 days old T3 transgenic chickpea seedlings were subjected to 100mM NaCl stress. The seedlings were harvested after 72hrs of NaCl stress and stored in RNA later for trancriptome analysis. A total of approx. 154 million raw reads were generated in all four samples with the total raw data of 49.14GB using Illumina Sequencer. 97493out of 214131 transcripts were observed as uniquely annotated, out of which 24767 genes were differentially expressed and highest number of DEGs were identified from membrane part i.e.15332 DEGs. Molecular function annotation of DEGs listed catalytic activity, transcriptional regulation activity, protein kinase activity, binding activity (heme, ADP), protein serine/threonine kinase activity with the highest members. Some significantly upregulated genes like aldehydehyde dehydrogenase, NADH dehydrogenase, histone, glutamine synthase, Calmodulin, Chlorophyll a,b binding protein, , MAPK, proline-rich protein, MYB48, MYB88, ABC transporter, GABA transporter, zinc finger protein and sucrose synthase were observed. These genes are already reported to play an important role in salt tolerance in various crops, but still more investigation is needed to understand the exact mechanism of salinity tolerance imparted by OsRuvB in transgenic chickpea.ThesisItem Open Access Simple sequence repeat analysis and micronutrient content in pearl millet [Pennisetum glaucum (L.) R. Br.](CCSHAU, 2017) Chaudhary, Rinku; Kharb, PushpaPearl millet [Pennisetum glaucum (L.) R. Br.] is highly cross-pollinated, diploid, photo-synthetically efficient C4 monocot plant belonging to family Poaceae. In present investigation, seventy three pearl millet inbred lines were evaluated for genetic variability in yield and its contributing traits, micronutrients (Fe and Zn) and crude protein content. Molecular diversity analysis was assessed using SSR primers. The analysis of variance indicated significant differences among all inbred lines for all the traits. A narrow difference was observed between PCV and GCV for all the traits suggesting the little role of environment in the expression of all these traits. A highly significant and positive correlation between grain Fe and Zn content was observed. Grain yield per plant showed no significant correlation with Fe, Zn and protein content suggesting improvement in nutrient value can be attempted without compromising on yield. Three inbred lines, H2302, HMP 808 and HBL 1121 were found to have high Fe, Zn and protein content as well as high yield. Inbred lines HMS 53B, HPT-2-12-59, H12/009, HMS 14B and H72-2-2 contained higher Fe and Zn than HBL 11, male progenitor of elite hybrids HHB 197, HHB 223, HHB 226, and thus may be used in developing superior hybrids. Out of 40 SSR primers used for screening, 19 were found to be polymorphic and used for molecular diversity assessment. A total of 46 alleles were amplified with 2.42 average allele per locus and 0.38 average PIC. At a similarity coefficient of 45%, seventy three inbred lines were grouped into two major clusters. HBL 0551 shared a very high genetic similarity with HBL 11 thus may be used as male parent in hybrid development programmes.
