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Theses

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2019 - 201912020 - 20211
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Subject: Zoology × Author: Sakshi × End date: 2021 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Effect of turmeric (Curcuma longa) supplemented feed on growth performance and hematological parameters on Pangasius hypopthalmus
    (CCSHAU, Hisar, 2021-09) Sakshi; Dharambir Singh
    The use of non-specific immunostimulants and plant products is gaining attention in aquaculture all over the world, which have beneficial effects for enhancing growth performance and improve immunity to control diseases in fishes. One of such herbs is turmeric (Curcuma longa), belonging to Zingiberaceae family, commonly used as an immuno-stimulant and an important ingredient in medicine. Pangasius is relatively new and fast-growing fish species for culture fisheries and has great potential to meet the growing demand of cheap quality food. The aim of present study was to evaluate the effect of turmeric (Curcuma longa) supplemented feed on growth performance, hematological and biochemical parameters of Pangasius hypopthalmus. The experiment was conducted in College of Fisheries Science, CCS HAU, Hisar for 90 days, using 500 L capacity glass aquaria and 240 fingerlings were randomly divided into 3 treatments and a control group. Three experimental diets were prepared by supplementing turmeric @3 gm/kg (T1), 6 gm/kg (T2) and 9 gm/kg (T3) with basal diet (mustard oil cake and rice bran) and control diet without turmeric. After 90 days of feeding trial, fishes fed with 6gm/kg turmeric (T2) exhibited significant (p0.05) increase in RBC, Hb, PCV and MCHC whereas significant increase in WBC counts was recorded. MCV decreased non-significantly (p>0.05) from initial to the final day along with MCH. Biochemical parameters like AST, ALT, ALP and blood glucose level were also decreased significantly (p<0.05) in treated fishes as compared to control group. Hence, the results revealed that supplementation of turmeric can improve the growth performance, hematological and biochemical parameters of catfish.
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    ThesisItemOpen Access
    Pesticides induced toxicity on biomolecules composition, immune cells and gut bacterial population of Pheretima posthuma
    (CCSHAU, 2019) Sakshi; Dharambir Singh
    Earthworms play important role in soil functions like soil aeration, stabilization of soil aggregates and water infiltration by their burrowing activity. The aforesaid activities are essential for maintaining the sustainability of agro ecosystem but they are decreasing and degraded because of indiscriminate use of pesticides. The earthworms ingest organically rich soil and release vermicast which harbour more bacteria than surrounding soil. This cast has buffering action for maintaining proper functioning of soil. The pesticide induces disruption in enzymatic activities and also affects the biomolecules composition of earthworms so present study was carried out to study the impact of pesticides toxicity on biomolecules compositions, immune cells and gut bacterial population of Pheretima posthuma. Both the pesticides viz. imidacloprid and pendimethalin were used individually as well as in combination for experimentation. Maximum reduction in biomolecule compositions was22.44%, 52.04% and 40.88% in crude protein, crude lipid, and carbohydrate respectively in earthworms treated with imidacloprid along with pendimethalin @ 0.60+2.50 μl/kg of substrate. There was significant reduction (72.28%) in coelomocytes, counted in earthworm exposed to highest concentration of imidacloprid along with pendimethalin @ 0.60+2.50μl/kg. Similarly, significant reduction was observed in number of granlocyte-I, granulocyte-II, mucocytes, lymphoidocytes and in vaculocytes. Maximum reduction inbacterial population inthe gut and vermicast was also observed in the same treatment. Among the two imidacloprid(insecticide) and pendimethalin (herbicide) imidacloprid was more toxic than pendimethalin when applied individually.