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Subject: Vegetable Science × Author: Rout, Bichhinna Maitri × End date: 2019 × Start date: 2019 × Type: Thesis ×
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    ThesisItemOpen Access
    Micropropagation and genetic fidelity testing in potato
    (CCSHAU, 2019) Rout, Bichhinna Maitri; Bhatia, A.K.
    The present experimental study was conducted to standardize an efficient protocol for rapid multiplication of two important cultivars (Kufri Neelkanth and Kufri Lima) and to check genetic fidelity using molecular markers. The findings showed that the surface sterilization with 0.2% Bavistin + 0.4% streptocyclin for 45 minutes followed by 0.1% HgCl2 treatment for 55 seconds was optimum for in vitro culture establishment and maximum survival percentage. In cv. Kufri Neelkanth the minimum number of days (3.3 days) required for bud initiation and highest number of buds/explant (3.5) were in MS media fortified with 0.5 mg/l BAP + 0.5 mg/l KIN while 3.2 days were required for initiation and 3.2 buds were formed in cv. Kufri Lima on the same. The maximum number of shootlets/explants (10.4) were recorded when auxins were used in combination with cytokinins at a concentration of 0.01 mg/l NAA + 0.25 mg/l KIN in cv. Kufri Neelkanth and 10.1 shootlets per explants were observed in cv. Kufri Lima. The number of days required for root initiation (2.1) were recorded to be minimum in rooting media consisting of MS medium+2.5 mg/l NAA in cv. Neelkanth and 2.2 days in cv. Kufri Lima. The maximum number of roots/explants (13.0) were observed when MS media fortified with 2.5 mg/l IBA in cv. Kufri Neelkanth while 12.6 number of roots were recorded on MS media fortified with 2.0 mg/l IBA in cv. Kufri Lima. Maximum 100 % rooting was observed in all MS media supplemented with different concentrations of auxins. The highest survival per cent (100%) of in vitro raised plants was recorded in potting media containing cocopeat, perlite and vermiculite in 3:1:1 ratio in both the cultivars. In vitro raised plants were assessed for genetic fidelity by using twenty RAPD primers. Out of twenty primers screened, two primers produced amplification while eighteen primers did not show any amplification in cv. Kufri Neelkanth whereas four primers produced amplification while sixteen primers did not show any amplification in. cv. Kufri Lima. DNA banding pattern of all tissue culture raised plants and mother plant was found similar showing monomorphism explaining that all the plants raised through tissue culture using shoot tips were true to type or genetically identical to the mother plant.