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  • ThesisItemOpen Access
    Antibacterial finish on cotton using Giloy extract
    (CCSHAU, 2019) Kumari Medha; Arya, Nisha
    The present study was intended to apply antibacterial finish on cotton fabric by using Giloy stem extract. To achieve the objectives projected in the research design, Giloy stem extract was selected for giving finishing treatment to cotton fabric. Pretreatment was given to cotton fabric prior to the application of finish using standardized concentration of Giloy stem extract with exhaust and pad dry cure methods. Giloy stem were extracted by cold aqueous maceration and soxhlet evaporation. It was found that percentage yield of extract was 18.2 per cent after 36 hours for Giloy stems. Bacteria (Bacillus spp. and Pseudomonas aeruginosa) were selected for the present study. The antibacterial activity of the extract was tested using agar well diffusion method. It was observed that the extract possessed antibacterial activity against Pseudomonas aeruginosa, but had no resistance against Bacillus spp. Hence, Pseudomonas aeruginosa was selected for further research. To study the efficacy of Giloy stem extract on cotton fabric, finished samples were tested for microbial resistance quantitatively by AATCC-100 test method before and after washing the samples (five wash cycles). The antibacterial finish applied with Giloy stem extract by exhaust and pad dry cure methods, was highly effective after 24 hours of inoculation against Pseudomonas aeruginosa. After 24 hours, antibacterial resistance gradually decreased and after 96 hours it reduced to 88.55 per cent and 89.33 per cent in cotton fabric sample finished by exhaust and pad dry cure methods, respectively. The cotton fabric sample finished with Giloy stem extract by exhaust and pad dry cure methods were analyzed for their bacterial resistance after five wash cycles. After 24 hours, the resistance gradually decreased and after 96 hours, it reduces to 87.44 per cent and 88.55 per cent by exhaust and pad dry cure methods, respectively. Thus, there was continuous decrease in antibacterial activity with increase in incubation period after washing but even then the finish was actively retained in washed samples after 96 hours. It was further concluded that after inoculation of Pseudomonas aeruginosa bacteria, microbes did not alter the physical properties of the finished samples heavily. Finished fabric was analyzed for change in physical properties and it was found that fabric weight, thickness, bulk and elongation of fabric were increased while fabric count, tensile strength, bending length, flexural rigidity, air permeability and moisture regain were decreased. After washing, physical properties of finished fabric were again investigated and it was found that fabric count, weight, thickness, bulk, bending length, flexural rigidity and tensile strength of Giloy stem extract finished sample decreased while elongation, air permeability and moisture regain increased. Conclusively, Giloy stem extract was observed as an efficient natural source for imparting antibacterial finish on textiles.