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Subject: Seed Science and Technology × Author: Chauhan, Davender Singh × End date: 2009 × Start date: 2003 ×
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    ThesisItemOpen Access
    Effect of ageing and priming on seed storage proteins of barley. (Hordeum vulgare L.)
    (CCSHAU, 2005) Chauhan, Davender Singh; Punia, R.C.
    Barley owing to its suitability for salinity and water stress tolerance was selected to define variability available in improved cultivars for seed vigor, viability and storability. An effort was also made to study the effect of ageing and priming on seed storage proteins on the basis of total seed protein profiles. To undertake the above studies six improved genotypes viz. Alfa-93, BH-393, BH-75, BCU-73, RD-2552, K-551 were taken define the changes in storage protein in fresh and accelerated aged seeds. It was observed that all the six genotypes were quite variable for seed quality parameters. The fresh seed lots of all the genotypes were having standard germination above 90%. However, accelerated ageing reduced it to nearly 50%, owing an adverse effect on all the seed quality parameters. On the basis of seed storage protein profiles of fresh and accelerated aged seeds it was revealed that protein profile of fresh seeds was less variable over accelerated aged seeds. An effort was also made to enhance the value of deteriorated seeds (accelerated aged) of BH-75 and BH-393 by giving priming treatments. It was revealed that priming with water and aqueous solution of PEG 6000 significantly increased the standard germination, rate of germination, dehdrogenase activity and vigor index by boosting the repair mechanism of seed. The protein profile of primed seeds revealed that priming results in hydrolysis of seed storage proteins.