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  • ThesisItemOpen Access
    Impact assessment of arbuscular mycorrhiza on chilli wilt caused by Fusarium oxysporum f. sp. capsici
    (CCSHAU, 2019) Sarita; Rakesh Kumar
    The present study was conducted to evaluave the impact assessment of arbuscular mycorrhiza on chilli wilt caused by Fusarium oxysporum f.sp. capsici. Surveys in different district of Haryana was conducted during 2017 - 2018 for mycorrhizal colonization (%), sporocarp number in soil and wilt intensity in the chilli and found that mycorrhizal colonization (per cent) ranged from 1 - 17.3 per cent and sporocarp number in soil ranged from 9 - 130. wilt intensity ranged from 0.5 - 7.9. The mycorrhizal per cent colonization and sporocarp number in soil were highest in Mahendragarh district (Ateli 17.3 % and 130 respectively), followed by Fatehabad district (Dani Binja Lamba 13.1 % and 91 respectively) and lowest in Hisar district (11.5% and 69 respectively). The maximum wilt intensity was recorded from the Fatehabad district (Bhuna, 7.9), followed by Mahendragarh (Silarpur, 7.3) and minimum from Hisar (Kharar-Alipur, 5.2). Three Glomus species were tested on chilli. Maximum plant growth parameters (Plant height, Root length, Dry weight of root and shoot), SPAD chlorophyll content, per cent mycorrhizal colonization and sporocarp number was maximum in Glomus intraradices, when 400 spores were inoculated followed by Glomus mosseae and Glomus fasciculatum. The maximum per cent disease control (57%) was recorded when F. oxysporum + G. intraradices + T. harzianum were inoculated. For the management of chilli wilt G. intraradices and T. harzianum were used with different combination. For the management of chilli wilt G. intraradices and T. harzianum were used with different combination. Maximum plant growth parameters (Plant height, Root length, Dry weight of root and shoot), SPAD chlorophyll content and NPK content was in triple inoculation (F. oxysporum + G. intraradices + T. harzianum) and minimum in F. oxysporum at 90 DAT. Different mycorrhizal species may differ in their ability to control the disease. For the desirable effect on plant growth and inducing resistance to pathogens, suitable host symbiont combination has to be found. The use of mycorrhizal fungi an effective method for disease control, mainly root and soil borne disease.
  • ThesisItemOpen Access
    Physiological responses for salinity tolerance in Indian mustard (Brassica juncea)
    (CCSHAU, 2017) Sarita; Sandooja, J.K.
    In this study, 15 genotypes of Brassica juncea namely RH0673, RH0725, RH1462, RH1470, RH1472, RH1501, RH1504, RH1507, RH1512, RH1514, RH1520, RH1535, RH8812, RH9304, and RH0305 which have unknown response to salinity were screened for the selection of salinity tolerant genotypes. Seeds of these genotypes were raised in plastic trays having 3kg soil saturated with 0, 8, 10, 12 and 14dSm-1 salinity salts with water. On the basis of this experiment, genotypes RH0725 and RH1512 were salinity tolerant whereas, genotypes RH1520 and RH8812 were susceptible to salinity. These four genotypes were sown in pots having 5kg dune sand saturating by 0, 8, 10 and 12dSm-1 salinity salts with water, for further investigation of physiological responses. Salinity stress affected all physiological parameters. Some physiological parameters like total chlorophyll content, chlorophyll fluorescence, photosynthetic rate, transpiration rate, protein content etc reduced due to salinity applications both at vegetative and flowering stage. However, some parameters like electrolyte leakage, MDA content, total soluble sugars antioxidant enzymes (CAT and POX) activity increased by all salinity levels both at vegetative and flowering stage. Yield and yield attributes such as no. of siliqua/plant, no. of seeds/siliqua and 1000 seeds weight were also reduced by all levels of salinity. Under salinity, minimum reduction in photosynthetic rate, total chlorophyll content, transpiration rate, total soluble proteins etc and maximum increase in osmolytes such as total soluble sugars, proline content etc were observed in genotype RH1512. So, genotype RH1512 performed very well under all salinity levels. So, it is concluded that, genotype RH1512 was more salinity tolerant where RH8812 was comparatively more sensitive towards salinity.