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20211
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Subject: Plant Pathology × End date: 2021 × Start date: 2020 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Studies on phytobiome, epidemiology and management of chickpea wilt caused by Fusarium oxysporum f. sp. ciceris
    (CCSHAU, Hisar, 2021-04) Khanna, Annie; Kushal Raj
    Present investigation entitled “Studies on phytobiome, epidemiology and management of chickpea wilt caused by Fusarium oxysporum f. sp. ciceris” indicated that early sowing of chickpea in the month of October ameliorate the disease severity in susceptible variety (JG 62 and C 235). Amongst weather variables Tmax, Tmin, and RHM were positively correlated with enhancing disease severity while relative humidity evening, wind, rainfall and rainy days were negatively correlated. On the basis of sequencing of 16S rRNA gene, the chickpea root bacterial endophytes belonged to genera Bacillus sp., Ochrobactrum sp., Luteimonas sp., Pseudomonas sp., Advenella sp., Paenibacillus sp., Cellulosimicrobium sp. and Microbacterium sp. Out of 20 chickpea root bacterial endophytes, CRBE1, CRBE3 and CRBE7 showed maximum antagonistic activity against Fusarium oxysporum f. sp. ciceris under in vitro conditions. The bacterial endophytes viz. CRBE2, CRBE3, CRBE4, CRBE7, CRBE8, CRBE10 and CRBE 17 showed positive reaction for siderophore production while CRBE1, CRBE7 and CRBE9 exhibited positive reaction for HCN production and CRBE1 showed highest growth on ACC supplemented medium. Minimum disease incidence and maximum phenol content (412.70μg/g) was observed in plants of chickpea variety HC 3 raised from seeds treated with CRBE3. Amongst plant extracts neem and datura while among fungicides, carbendazim 50 WP and azoxystrobin 23 SC were found to be best under in vitro and field conditions. Among bio-agents evaluated T. viride and T. harzianum were found to be superior to other bioagents in inhibiting mycelial growth, minimizing disease incidence and increasing seed yield of chickpea variety JG 62. The soil amended with G. mosseae reduced disease incidence and enhanced seed yield and yield attributes viz. number of pods per plant, number of seeds per pod and seed weight in chickpea varieties JG 62 and C 235.