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20171
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Subject: Plant Pathology × Author: Amoghavarsha Chittaragi × Start date: 1999 ×
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    ThesisItemOpen Access
    Evaluation of various lignocellulosic products for the cultivation of shiitake mushroom [Lentinula edodes (Berk.) Pegler]
    (CCSHAU, 2017) Amoghavarsha Chittaragi; Ashwani Kumar
    The present studies were carried out to evaluate the various lignocellulosic products for the cultivation of shiitake mushroom [Lentinula edodes (Berk.) Pegler] on locally available substrates. The study on different media revealed that highest growth rate after 6 days of incubation was recorded on the sorghum meal agar with strain OE-388S (69.9 mm) whereas lowest growth rate was observed with strain LE-16-02 on PDA (39.5 mm). Evaluation of the mycelial growth of L. edodes on the different agro-residues was studied on several agro-residues and sorghum straw showed the fastest colonization of the shiitake (strain OE-388S) within 25.6 days and the slowest rate of colonization was exhibited by saw dust + sorghum straw combination (44.6 days). Study on the optimization of the different spawning substrates of L. edodes was conducted using various grains and the fastest coverage of mycelia was observed on the sorghum grains (20 days) whereas slowest mycelial run was observed on the pearl millet grains (45 days). In the experiment for evaluation of the different substrate sterilization/pasteurization methods, two substrates i.e. sawdust and pearl millet straw were taken up and found that the autoclaving for 90 min at 15 psi showed the best result with only 3.7 per cent mould incidence on the saw dust and 6.2 per cent incidence on the pearl millet straw. In the study for standardization of low cost seasonal cultivation, the best results were obtained on wheat straw supplemented with 15 per cent wheat bran.