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  • ThesisItemOpen Access
    In Vitro And In Vivo Selection Of Wheat Genotypes For Salinity Tolerance And Their Characterization By Molecular Markers
    (Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2005) Lokesh; Behl, R. K.
  • ThesisItemOpen Access
    Phenotypic Stability And Genetic Diversity In Pea ( Pisum Sativum L.)
    (Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2005) Rakesh Kumar; Ram Dhari
  • ThesisItemOpen Access
  • ThesisItemOpen Access
    Characterization Of Wheat Genotypes For Grain Quality Traits Using Molecular Markers
    (Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2005) Somveer; Behl, R.116, xxiii K.
  • ThesisItemOpen Access
    Variability among disomic chromosome substitution lines of wheat for micronutrients uptake
    (CCSHAU, 2005) Subodh Kumar Singh; Behl, R.K.
    The materials of present investigation consisted of two parents Viz. Chinese spring and C591 and twenty disomic chromosome substitution lines of C591 in the background of Chinese Spring. The experiment was laid out in completely randomized block design with three treatments and replicated thrice in pots under screen house conditions. The data were recorded for different plant morphological characters, macro (nitrogen, phosphorus) and micronutrients (iron, manganese, zinc, copper) contents and their uptake and Azotobacter viable count in rhizosphere of all disomic lines and their parents. 115 Analysis of variance revealed the presence of significant variability in the materials under study. Observations recorded on yield and its attributing characters and macro and micronutrients contents as well as their plant uptake revealed genotype and chromosome specific variation. On the basis of genotypic performance under various treatments for different plant characters, macro and micro nutrients uptake and Azotobacter colony count, disomic chromosome substitution lines 1D, 5D, 6B, 7B figured promising. It could be postulated that the quantitative traits loci for high micro nutrients uptake and response to Azotobacter inoculation were present on 1D, 5D, 6B, 7B chromosomes. This indicated good scope for wheat improvement through analytical breeding at chromosome level. Correlation coefficients revealed highly significant to significant positive association among most characters. Highest magnitude of correlation coefficient was noticed between 250 grain weight and yield per plant and lowest for number of tillers and Zn uptake However, spike length and Cu content did not show significant association in some cases.
  • ThesisItemOpen Access
    Characterization of cytoplasmic male sterile lines in wheat (Triticum aestivum L.)
    (CCSHAU, 2006) Rana, Jyoti; Sethi, S.K.
    The present investigation was carried out during 2005-2006 at experimental area of wheat section, Department of Plant Breeding, CCS Haryana Agricultural University, Hisar. In this experiment various floral and agronomic traits and protein profiles of 16 cytoplasmic male sterile lines (A lines) of wheat representing two different cytoplasmic sources and their corresponding maintainer lines (B lines) were studied under timely and late sown conditions. Significant variation between the CMS lines and B lines was observed with respect to floral traits viz., anther size, filament length, ovary size, length of style and anther colour, glumes opening angle, stigma receptivity, pollen viability, out crossing and agronomic traits namely number of tillers, plant height, spikelets, number of spikelets per spike. The mean values for different floral traits in the CMS lines was lesser than an equal to that of their fertile counterparts opened. Glumes angle had maximum variation, stigma receptivity had the least variation, which ranged from 4 to 5 days. Among various CMS lines all the CMS lines except three exhibited complete male sterility under both dates of sowing. The number of tillers per plant and plant height was reduced in CMS lines as compare to maintainer lines. For traits like spike length, number of spikelets per spike, reduction was in both direction negative and positive. In this way, the interaction of cytoplasm with different nuclear backgrounds can be in positive as well as in negative direction. CMS lines flowered later than respective B lines. In electrophoresis a polymorphic band was observed while it was absent in majority of CMS lines and present in all the maintainer lines. The result of present study indicate more or less stable performance of sixteen CMS lines studied with respect of floral, agronomic traits and protein profile.
  • ThesisItemOpen Access
    Studies on drought tolerance, genetic divergence and character association in mothbean [Vigna aconitifolia (Jacq.) Marechal]
    (CCSHAU, 2006) Ranjit Singh; Jaivir Singh
    The objectives of the present study were to study the physiological basis of drought tolerance in mothbean, to determine the yield component under dry land conditions, to identify diverse and elite genotypes and suggesting breeding method for further improvement in mothbean. The experiment was conducted during Kharif, 2002 at research area, CCS Haryana Agricultural University, Hisar. In the present investigation, 43 genotypes of mothbean were grown in randomized block design with four replication accommodating spacing 30× 10 cm. Observation on 5 competitive plants for recording of data on different physiological traits and yield components were taken. Substantial amount of coefficient of variation was observed for all the traits/characters under study. A wide range for different traits/characters were also observed. The higher phenotypic coefficient of variation over genotypic coefficient of variation showed in almost all the traits/characters. The estimation of -iv- -4- high heritability with high genetic advance was recorded for pod per plant in both the environments while in case of physiological traits it was observed for water retention. On the basis of D2-value 43 genotypes were grouped into nine cluster in E1 and eight in E2. There was no association between clustering pattern and ecogeographical distribution of the genotypes. RMO 257, RMO 40 and Jwala are released varieties were clustered into same cluster in both the environment. In almost all the cases phenotypic correlation coefficient was more than genotypic correlation coefficient. Correlation studied revealed significant positive association of seed yield per plot with 100-seed weight, seed yield per plant, pod per plant, pod per cluster in both environment. Path analysis revealed that maximum direct effect on seed yield per plant was through yield per plot and pod per cluster in E1 and E2 respectively. An examination of the trend of correlation revealed that the yield per plot, pod per plant, pod per cluster, pod length and 100-seed weight were the important characters that exerted the greatest influence both directly and indirectly upon seed yield per plant. These characters were important components in almost all correlations that involved seed yield. On basis of membrane stability the best performing genotypes/varieties were IC 329051, IC 311448, IC 329044 and RMO 257. On basis of the canopy temperature depression the best performing genotypes/varieties were IC 329051, IC 311448, IC 329044, IC 329090, IC 370471, IC 329037, IC 311427 and RMO 40. RMO 40 and RMO 257 were observed as best performer for high water retention.
  • ThesisItemOpen Access
    Genetic divergence analysis in indian mustard (brassica juncea (l.)czern & coss) germplasm
    (CCSHAU, 2006) Goyat, Binesh; Dhiraj Singh
    Two hundred germplasm lines were evaluated for genetic divergence. The experiment was laid in augmented design. The highest intra cluster D2 values was observed for cluster 3, with euclidean distance (4.23). The inter cluster distance have been found to be highest between cluster 1 and 6 euclidean distance (5.546). The cluster mean values were observed maximum in cluster 6 for no. of primary branches per plant, main shoot length, no. of siliqua on main shoot and oil content. Genotypic and phenotypic variances and coefficient of variation revealed substantial genetic diversity in the material. High heritability was observed for no. of seeds per siliqua and main shoot length. Genetic advance as per cent means were high for seed yield, no. of primary branches and no. of secondary branches per plant. Seed yield per plant showed positive significant correlation for no. of primary branches, no. of secondary branches, siliqua on main shoot. No. of secondary branches showed maximum positive direct effect for seed yield. Promising germplasm lines based upon different quantitative characters where identified. The use of such identified lines is advocated for developing superior genotypes/hybrids.
  • ThesisItemOpen Access
    Studies on combining ability, heterosis and character association in sunflower (Helianthus annuus L.)
    (CCSHAU, 2005) Kaushal Kumar; Sangwan, R.S.
    Seventy-seven genotypes comprising 16 parents (6 females and 10 males), their 60 F1 hybrids and one check HSFH-848 were grown in randomized block design with three replications to study the combining ability, heterosis and character association in sunflower. The analysis of variance indicated the presence of sufficient genetic variability for eleven character studied. Among female parents, CMS 234A and CMS 338A and among male parents, RHA 274, RHA 271 and RHA 298-118 showed high gca effects for seed yield and some of the yield attributing traits. The cross CMS 234 x RHA 298-118 showed high per se performance high sca effects and high heterosis for seed yield. The estimates of correlation coefficient indicated the positive association between days to 50% flowering and days to maturity and negative association between oleic acid and linoleic acid. Seed yield was highly and positively associated with number of seeds per plant, head diameter, plant height, 100-seed weight and stem girth and negatively associated with oil content.