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Identification and Mass Multiplication of Steinernema and Heterorhabditis species indigenous to Haryana
(CCSHAU, 2015) K.V.V.S., K. Kranti; Walia, K.K.
Identification and mass multiplication of indigenous isolates of entomopathogenic nematodes was attempted. Of the fifteen populations studied- fourteen belonged to genus Steinernema and one to genus Heterorhabditis. Six new species S. numeropapillatum n. sp., S. oryzae n. sp., S. sirsaense n. sp., S. indicum n. sp., S. gangulyi and S. hisarense n. sp. were identified. First four species were with horn like structures in cephalic region of IJs which was absent in last two species. Other species identified were H. indica Poinar et al. 1992, S. pakistanense Shahina et al. 2001 (three populations), S. bicornutum Tallosi et al. 1995, S. ceratophorum Jian et al. 1997, S. siamkayai Stock et al. 1998. SEM studies of S. oryzae n. sp. and S. sirsaense n. sp. revealed the lateral field pattern as 2,4,6,8,6,4,2 and 2,4,6,4,2, respectively and also the number of genital papillae as 25. Of the eight media tested, S. abbasi ans H. indica both multiplied maximum on modified dog biscuit medium. Wout‟s medium was second best in IJs yield. Yield was maximum at 30 days and reduced drastically at 60 days. S. abbasi yielded maximum at 25 ºC whereas H. indica multiplied maximum at 30 ºC. None of the species multiplied at 15 ºC.