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Subject: Nematology × Author: Deepak Kumar × Start date: 2022 × Type: Thesis ×
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    ThesisItemOpen Access
    Studies on the effect of various factors on survival and efficacy of Heterorhabditis spp. against brinjal shoot and fruit borer, Leucinodes orbonalis Guenee
    (Chaudhary Charan Singh Haryana Agricultural University hisar, 2022-11) Deepak Kumar; Anil Kumar
    Present investigations were carried out on “Studies on the effect of various factors on survival and efficacy of Heterorhabditis spp. against brinjal shoot and fruit borer, Leucinodes orbonalis Guenee” under laboratory and field conditions in Department of Nematology, CCS HAU, Hisar between years, 2019 to 2022. Entomopathogenic nematodes (EPNs) infestations were found in 40 of the 160 soil samples that were collected from eight districts of Haryana during survey. On the basis of corpse colour, 25 of the 40 soil samples tested positive for Steinernema species and eight of those contained Heterorhabditis species. The highest frequency of occurrence of EPNs was found in Panipat (40.9%) followed by Hisar (37.5%) and Sonipat district (36.4%), while Karnal district had the lowest incidence of EPNs i.e. 19 percent. Among the four temperature tested for storage, both of the effective isolates of H. indica (D-2 & D-6) had greater viability at a temperature of 25º C. The highest multiplication of IJs of H. indica D-2 and H. indica D-6 was observed in modified dog biscuit medium followed by modified wout’s medium-III. Survival and activity of EPNs decreased gradually in glycerin and liquid paraffin antidesiccants in 90 days storage. The active IJs obtained from glycerin @ 1 percent storage, showed maximum efficacy against the test insect in both the isolates of H. indica (D-2 & D-6). When H. indica isolates were applied in two spray treatments with a three-day interval, the maximum mortality of Leucinodes orbonalis was seen and this was considerably comparable with spinosad 45 SC @ 72ml per acre was applied as a treatment for the management of insect pests of brinjal in field conditions. So, the native isolates of H. indica (D-2 & D-6) proved virulent and effective against brinjal shoot & fruit borer in field conditions. Hence, it was concluded from the study that H. indica isolates are environmentally safe alternate to chemical pesticides in brinjal.