Thumbnail Image

Theses

Browse

20181
Reset filters
Subject: Microbiology × End date: 2018 × Start date: 2018 × Type: Thesis × Thesis Type: M.Sc ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    Biocontrol of root-knot nematode (Meloidogyne javanica) in brinjal (Solanum melongena L.) using plant growth promoting rhizospheric bacteria
    (CCSHAU, 2018) Antil, Sonam; Rakesh Kumar
    Egg plant (Solanum melongena L.) or brinjal is a nightshade species belonging to family Solanaceae and is the second most important vegetable crop after tomato and shows high susceptibility to infection by the root knot nematode Meloidogyne javanica. Management of the root-knot nematodes via biological methods such as PGPR is a novel technology emerging for sustainable agriculture. In this context, a total of 63 isolates were isolated from nematode affected rhizospheric soil of brinjal, tomato crops and earthworm culture media. All the 63 isolates were characterized for various plant growth promoting traits. Out of 63, fifteen isolates produced siderophores and maximum production was observed by isolate LBB-1and KMT-2. Chitinase activity was observed in 14 isolates and isolates KMT-2 and KMT-8 showed maximum chitinase activity. Sixteen isolates were found to be positive for HCN production and maximum production was seen in isolates NAE-1, KMB-3, KMT-2 and KMS-6. Fifty seven isolates were capable of excreting ammonia and maximum ammonia excretion was observed in KMT-2 (4.8μg/ml) and LBB-1 (4.7 μg/ml). All the bacterial isolates except one were producing IAA and high level of IAA production was observed in KMT-1 (45.9 μg/ml), KMB-2 (38.8 μg/ml), KMB-4 (27.7μg/ml) and KMT-5 (26.4μg/ml). A total of 39 isolates solubilized phosphate and highest P-SI was observed in isolate KMT-1(2.0) and LBB-4 (1.7). Twelve isolates- NAB-4, NAS-1, NAE-1, LBB-1, LBS-1, KMB-3, KMT-2, KMT-4, KMT-5, KMT-8, KMS-3 and KMS-6 resulted in minimum hatching in egg masses and higher mortality rate in J2 of M. javanica (upto 92%). Based on all the screening tests, a total of 12 were selected for pot house experiment on brinjal crop. Rhizospheric microbial count increased in all treatments from 0 DAP to 45 DAP. Under pot house conditions, T14 (RDF +KMT-4), T15 (RDF +KMT-5), T16 (RDF + KMT-8) and T17 (RDF +KMS-6) showed maximum plant growth, reduced galls and eggs in roots, and final nematode population in soil.