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Colonisation behaviour of gluconacetobacter diazotrophicus in cotton plant
(CCSHAU, 2006) Chawla, Niti; Bansal, R.K.
Studies on the colonisation behaviour of Gluconacetobacter diazotrophicus 35-47 introduced as seed treatment was undertaken in root-knot nematode (Meloidogyne incognita) infected and healthy cotton (Gossypium hirsutum) cv. H-1098 plants grown in sterilized and natural field soil in green house. G. diazotrophicus 35-47 inoculation significantly reduced root-knot disease intensity and also enhanced plant growth parameters of both nematode infected and healthy cotton plants in sterilized as well as natural field soil. Studies on colonisation behaviour of the bacterium has indicated that it colonised root surface and not the internal tissues of the cotton plant, suggesting that it is a ‘root-surface coloniser’ and not the ‘endophyte’ of cotton. From the results, it is also evident that bacterium could grow upto last day of observation (90 DAS) of plant growth in natural field soil in healthy plants, however, nematode infection reduced bacterial multiplication. Optimisation study on the cotton root surface sterilisation showed that exposure in 10% sodium hypochlorite (containing 4% free chlorine) solution for 15 minutes followed by 5-6 times rinsing in sterile distilled water is sufficient to achieve complete surface sterility.