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20222
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Subject: Genetics and Plant Breeding × End date: 2022 × Start date: 2022 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Effect of different plant growth regulators, sugars and gelling agents on in-vitro multiplication and different potting mixtures on survival rate of in-vitro raised plants of Brahmi [Bacopa monnieri (L.)]
    (CCS HAU, Hisar, 2022-07) Manish Kumar; Dahiya, G.S.
    The investigation was carried out to study the effect of different plant growth regulators, sugars and gelling agents on in-vitro multiplication and different potting mixtures on survival rate of in-vitro raised plants of Brahmi [Bacopa monnieri (L.)]. For in-vitro multiplication plant cultures were inoculated on MS media supplemented with different plant growth regulators (BAP/Kn and IAA/NAA), carbon sources (fructose, sucrose, glucose, table sugar and maltose), gelling agens (agar-agar, agarose, gelrite, guargum and isabgol). The maximum shoots were observed on MS medium supplemented with M2 (4.6±0.67), M12 (5.0±0.04), M32 (4.9±0.06) and M19 (5.0±0.19). The maximum number of shoots (11.8±0.22) observed when MS media is supplemented with table sugar. The very good growth/rooting was observed on ½ basal media. The well rooting plantlets were tansplanted into pots containing various components of soil, sand, vermicompost, FYM, coco pit and perlite. The maximum (100%) survival were observed in four different mix including sand : soil : FYM, sand : soil: vermicompost, sand : soil : FYM : vermicompost and coco pit : vermicompost : perlite under green house conditions. Hardened plants were successfully transferred to the field conditions.
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    ThesisItemOpen Access
    Morphological and Biochemical characterization of elite lines of upland cotton (Gossypium hirsutum L.)
    (CCSHAU Hisar, 2022-06) Saraswat, Himanshi; Sonika
    The present investigation “Morphological and Biochemical characterization of elite lines of upland cotton (Gossypium hirsutum L.)” was carried out with 50 elite lines of upland cotton for the morphological characterization of genotypes as per DUS testing, assessment of genetic variability among genotypes, analysis of biochemical parameters, and correlation analysis of seed cotton yield per plant with its contributing traits. The research was conducted at farm research area, cotton section, Department of genetics and plant breeding, CCS HAU, Hisar. The experimental design adopted for present study randomized block design with three replications. Thirty-five morphological and five biochemical parameters were taken into consideration to fulfil the purpose of research. Morphological traits viz., hypocotyl pigmentation, leaf characteristics, stem characteristics, plant height, plant growth habit, days to first flower, floral traits, boll features showed significant amount of variation, stable and uniform expression of traits among replications, which revealed that they can be used for the categorization of elite genotypes into distinct classes. Analysis of variance observed for different quantitative characters and biochemical parameters was highly significant for all the characters showing enough magnitude of genetic variability present among genotypes. Assessment of genetic variation in terms of GCV (%), PCV (%), heritability in broad sense (%) and genetic advance % mean for all the traits under study revealed that high magnitude of GCV & PCV along with high heritability coupled with high genetic advance as % mean was present in seed cotton yield per plant, which is highly beneficial for the genetic improvement of the trait. Low magnitude of GCV & PCV was shown by days to first flower, number of seeds per locule as well as protein content. High heritability coupled with high genetic advance as % mean was found in gossypol content, sugar content, phenol content and tannin content of cotton seed of all the genotypes under investigation. Correlation analysis of seed cotton yield per plant with its contributing traits at both genotypic and phenotypic level revealed that the association of boll weight(g), number of bolls per plant, seed index(g), lint index(g), plant growth habit and number of monopods per plant was highly significant and positive which indicated that the improvement in these characters will ultimately result in the genetic improvement of yield. Number of locules per boll and tannin content was significantly and negatively correlated with seed cotton yield per plant which indicated that these two traits are not suitable for indirect selection for the improvement of yield.