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Open Access
Effect of different plant growth regulators, sugars and gelling agents on in-vitro multiplication and different potting mixtures on survival rate of in-vitro raised plants of Brahmi [Bacopa monnieri (L.)]
(CCS HAU, Hisar, 2022-07) Manish Kumar; Dahiya, G.S.
The investigation was carried out to study the effect of different plant growth regulators, sugars and gelling agents on in-vitro multiplication and different potting mixtures on survival rate of in-vitro raised plants of Brahmi [Bacopa monnieri (L.)]. For in-vitro multiplication plant cultures were inoculated on MS media supplemented with different plant growth regulators (BAP/Kn and IAA/NAA), carbon sources (fructose, sucrose, glucose, table sugar and maltose), gelling agens (agar-agar, agarose, gelrite, guargum and isabgol). The maximum shoots were observed on MS medium supplemented with M2 (4.6±0.67), M12 (5.0±0.04), M32 (4.9±0.06) and M19 (5.0±0.19). The maximum number of shoots (11.8±0.22) observed when MS media is supplemented with table sugar. The very good growth/rooting was observed on ½ basal media. The well rooting plantlets were tansplanted into pots containing various components of soil, sand, vermicompost, FYM, coco pit and perlite. The maximum (100%) survival were observed in four different mix including sand : soil : FYM, sand : soil: vermicompost, sand : soil : FYM : vermicompost and coco pit : vermicompost : perlite under green house conditions. Hardened plants were successfully transferred to the field conditions.
Open Access
Morphological and Biochemical characterization of elite lines of upland cotton (Gossypium hirsutum L.)
(CCSHAU Hisar, 2022-06) Saraswat, Himanshi; Sonika
The present investigation “Morphological and Biochemical characterization of elite lines of upland cotton (Gossypium hirsutum L.)” was carried out with 50 elite lines of upland cotton for the morphological characterization of genotypes as per DUS testing, assessment of genetic variability among genotypes, analysis of biochemical parameters, and correlation analysis of seed cotton yield per plant with its contributing traits. The research was conducted at farm research area, cotton section, Department of genetics and plant breeding, CCS HAU, Hisar. The experimental design adopted for present study randomized block design with three replications. Thirty-five morphological and five biochemical parameters were taken into consideration to fulfil the purpose of research. Morphological traits viz., hypocotyl pigmentation, leaf characteristics, stem characteristics, plant height, plant growth habit, days to first flower, floral traits, boll features showed significant amount of variation, stable and uniform expression of traits among replications, which revealed that they can be used for the categorization of elite genotypes into distinct classes. Analysis of variance observed for different quantitative characters and biochemical parameters was highly significant for all the characters showing enough magnitude of genetic variability present among genotypes. Assessment of genetic variation in terms of GCV (%), PCV (%), heritability in broad sense (%) and genetic advance % mean for all the traits under study revealed that high magnitude of GCV & PCV along with high heritability coupled with high genetic advance as % mean was present in seed cotton yield per plant, which is highly beneficial for the genetic improvement of the trait. Low magnitude of GCV & PCV was shown by days to first flower, number of seeds per locule as well as protein content. High heritability coupled with high genetic advance as % mean was found in gossypol content, sugar content, phenol content and tannin content of cotton seed of all the genotypes under investigation. Correlation analysis of seed cotton yield per plant with its contributing traits at both genotypic and phenotypic level revealed that the association of boll weight(g), number of bolls per plant, seed index(g), lint index(g), plant growth habit and number of monopods per plant was highly significant and positive which indicated that the improvement in these characters will ultimately result in the genetic improvement of yield. Number of locules per boll and tannin content was significantly and negatively correlated with seed cotton yield per plant which indicated that these two traits are not suitable for indirect selection for the improvement of yield.
Open Access
Characterization for yield and quality traits under aerobic and transplanted condition in rice (Oryza sativa L.)
(CCSHAU, Hisar, 2021-09) Bhawana; Rakesh Kumar
The present experiment was carried out at Rice Research Station, Kaul, CCS Haryana Agricultural University Hisar, during Kharif, 2020 to estimate co-efficient of variability, heritability, genetic advance as percent of mean, character association, path co-efficient analysis and genetic divergence for yield and quality traits grown under aerobic and transplanted conditions. A total of 48 genotypes (24 basmati and 24 non-basmati genotypes) were grown in single row (5m long) of each genotype in each replication using randomized block design with spacing 20 x15 cm between rows and plants respectively. Observations were recorded on five randomly selected plants for each genotype on 21 morphological and quality traits. Mean sum of squares due to genotypes were highly significant for all the traits indicating the presence of sufficient variability under both the conditions. Narrow range of GCV and PCV indicated major role of genetic factors in expression of traits than environmental factors. In basmati genotypes, high heritability coupled with high genetic advance as per cent of mean was observed for grain yield/plant, plant height, number of grains/panicle and root dry weight under both conditions. While in non-basmati genotypes, grain yield/plant had high heritability coupled with high genetic advance as percent of mean revealed the importance of additive gene action in expression of the trait. Grain yield/plant showed significant positive correlation with number of tillers/plant, test weight, harvest index % and kernel breadth under both conditions for basmati genotypes, whereas in non-basmati genotypes grain yield/plant exhibited significant positive correlation with biological yield/plant followed by harvest index %, plant height, test weight , number of grains/panicle, number of tillers/plant, root fresh weight and root volume under both conditions suggesting that for improving grain yield direct selection of genotypes for these traits will be more fruitful. Path analysis revealed that biological yield/plant and harvest index % exhibited major positive direct effect on grain yield under both conditions for basmati genotypes whereas, in non-basmati genotypes kernel length followed by number of grains/panicle, days to 50% flowering and biological yield/plant, harvest index % and root volume exerted high positive direct effect on grain yield. Group constellation of genotypes under both the conditions (aerobic and transplanted) did not follow their geographical distribution. On the basis of grain yield HKR 15-455, HKR 16-459, Pusa 1557-06-08-126-162-156 and HKR 15-488 were identified superior than check Haryana Basmati -2 whereas HKR 15-488 and HKR 16-459 were identified as suitable genotypes for quality parameters over check Taraori Basmati. Genotypes, IR14L521, HKR 16-35 and NVSR 2107 were observed suitable under both aerobic and transplanted conditions as these genotypes performed significantly higher than the check HKR 47 and HKR 48 respectively.
Open Access
Evaluation of bread wheat genotypes for drought and heat tolerance under field conditions
(CCSHAU, Hisar, 2021-07) Madhu Kumar K G; Somveer
A field experiment was conducted in the research area of Wheat and Barley Section, Department of Genetics and Plant Breeding, CCSHAU, Hisar, during the rabi season of 2019-2020 to evaluate 62 bread wheat genotypes under normal, drought, heat and combined stress conditions. Magnitude of variability, character association, path analysis, genetic diversity and cluster analysis for various morpho-physiological characters were studied. The experiment was conducted in four different environments, viz., normal sown-irrigated (normal), normal sown-rainfed (drought), late sown-irrigated (heat) and late sown-rainfed (combined stress) and was laid out in RBD with two replications in each environment. Analysis of variance revealed highly significant variation among the genotypes for all the characters studied. Moderate estimates of GCV and PCV were reported for traits- early vigour, spike weight, number of grains/spike, grain weight/spike, grain yield/plant, harvest index and CTD, accompanied by moderate to high heritability and genetic advance as per cent of mean in all four environmental conditions, which suggests that still there is scope for further improvement of genotypes for these characters. Grain yield per plant reduced by 24.8%, 14.8% and 36.4% due to drought, heat and combined stress, respectively. Grain yield had a positive and significant correlation with number of productive tillers/plant, grain weight/spike, spike weight, number of grains/spike, 1000-grain weight, biological yield, harvest index, chlorophyll content, CTD and NDVI. Path coefficient analysis revealed that harvest index had the highest positive direct effect towards grain yield per plant, followed by biological yield per plant in all four environments. Grain weight/spike in normal and drought stress and number of grains/spike in heat and combined stress had high positive direct effects on grain yield. Thus, these traits may be used as selection criteria for respective environments. Clustering using Ward’s method grouped 62 genotypes into 6, 8, 6 and 7 distinct clusters under normal, drought, heat and combined stress respectively. The highest inter-cluster distance was found between clusters 3 and 4 (9.908), clusters 4 and 8 (9.713), clusters 1 and 4 (8.269) and clusters 3 and 6 (8.876) in normal, drought, heat and combined stress respectively, representing maximum variation between them. Clusters 4, 8, 4 and 6 in normal, drought, heat and combined stress, respectively, recorded the highest cluster means for many of the yield attributing traits, indicating the superior performance of genotypes in these clusters. Genotypes- P 13855, P 13841, P 13666, P 13633, P 13821 had low DSI, genotypes- P 13789, WH 1025, P 13633, P 13644, P 13858 had low HSI and genotypes- DBW 187, HD 3086, P 13815, P 13789, P 13841 had low CSSI values, subsequently they were categorised as drought-tolerant, heat tolerant and combined stress tolerant genotypes, respectively. Among seed quality parameters, standard germination was significantly influenced by parent-plant growing conditions. Drought stress alone reduced the standard germination by 7.9%. The decreased vigour indices in stress environments were particularly due to decreased standard germination. High EC of seed leachates was observed for seeds from plants subjected to drought stress alone.
Open Access
Genetic diversity and path analysis studies in sorghum for dual type characters
(CCSHAU, Hisar, 2021-07) Kamal Singh; Jindal, Yogesh
The present study was carried out on thirty sorghum genotypes for assessing genetic diversity and path analysis for dual type characters on the basis of 28 traits viz. morphological, biochemical, qualitative and yield traits. Sorghum genotypes were categorized on the basis of various qualitative traits which included leaf midrib color, days to 50% flowering, plant height, leaf length, leaf breadth, panicle shape, panicle length, panicle density at maturity, anther color, awns presence, grain color and grain lusture. Analysis of variance (ANOVA) revealed highly significant differences for all 21 quantitative traits included in the investigation. Maximum green fodder yield, dry fodder yield, stover yield and grain yield were reported for genotypes SH 1560, SH 1485, SH 1952 and SH 1955 respectively. Coefficient of variation (GCV and PCV) were high for leaf stem ratio, panicle length, TSS, crude protein yield, HCN, green fodder yield, dry fodder yield, stover yield and harvest index; while low for days to 50% flowering, leaf length, days to maturity and crude protein. High heritability along with high genetic advance was recorded for most of traits like plant height, internode length, leaf stem ratio, stem girth, panicle length, TSS, crude protein yield, HCN, tannin, green fodder yield, dry fodder yield, stover yield, grain yield, test weight and harvest index. Green fodder yield showed positive significant correlation with days to 50% flowering, plant height, internode length, no. of leaves per plant, leaf length, stem girth and days to maturity; dry fodder yield showed positive correlation with no. of leaves per plant, TSS and crude protein yield while grain yield showed positive significant correlation with tannin only. Path analysis revealed that plant height, no. of leaves per plant, leaf length, leaf breadth, leaf stem ratio, days to maturity, crude protein yield, grain yield and test weight were having positive direct effect on dry fodder yield while negative direct effect was shown by days to 50% flowering, internode length, stem girth, panicle length, TSS, crude protein, HCN, tannin, green fodder yield, stover yield and harvest index. In D2 statistics, thirty genotypes were clustered into five different clusters. Among these clusters, Cluster-I was largest followed by cluster II and cluster IV having 19, 5 and 4 genotypes respectively. The maximum inter-cluster distance was recorded between cluster II and cluster III.
Open Access
Studies on genetic variability for drought tolerance in advance lines of wheat (Triticum aestivum L. em. Thell)
(CCSHAU, Hisar, 2021-08) P Arun, Nithya; Om Parkash, Bishnoi
The present investigation consists of 40 advance lines of wheat raised in RBD design with 3 replications in the Rabi 2019-20 at Research Farm Area of Wheat and Barley section, Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar, with the objective of assessment of the genetic variability, heritability, genetic advance, correlation and path analysis. Observations were recorded for 16 morpho-physiological traits viz., days to heading, days to anthesis, days to maturity, plant height (cm), number of effective tillers per plant, spike length (cm), number of spikelets per spike, number of grains per spike, biological yield per plant (g), grain yield per plant (g), harvest index (%), thousand grain weight (g), NDVI, SPAD, CTD and DTI under irrigated and drought sown conditions. The findings of the analysis of variance showed that there was significant genetic variability in grain yield and its contributing traits. The magnitude of PCV estimations were greater than that of comparable GCV estimates, implying that environmental factors were also influencing genotype expression. The combined analysis of correlation coefficient analysis and path coefficient analysis revealed that the characters, namely, harvest index, biological yield per plant, thousand grain weight, number of grains per spike, NDVI, SPAD and CTD were the major yield attributing characters and hence; emphasis should be given to these traits while programming any plant breeding programme to develop drought tolerant cultivars. On the basis of drought tolerance index advance wheat lines viz., BRW 3806, C 306, NW 7049, WH 1164, PBW 757, DBW 88, DBW 221, DBW 302, DBW 187, WH 1124, HD 3347, HD 3271 and PBW 824 were found to be tolerant to drought stress. So, these lines can be used for further breeding programme for development of drought tolerant wheat varieties.
Open Access
Molecular and Morphological studies in F5 generation of wheat derived from WH 711 and PBW 698 for Yellow rust resistance
(CCSHAU, Hisar, 2020-08) Mohammadi, Lina; Mukesh Kumar
Open Access
Studies on genetic variability and association analysis for various morphological and quality traits in sugarcane (Saccharum spp.Complex) clones
(CCSHAU, Hisar, 2021-09) Hardeep; Sharma, Sudhir
In present investigation, 50 sugarcane genotypes were evaluated in randomized block design with three replications at the research farm of CCS HAU, RRS, Uchani, Karnal during Spring 2020. This study was intended to determine genetic variability, correlation and path analysis for seventeen characters among fifty early maturing sugarcane clones. Analysis of variance showed that mean sum of squares due to genotypes were highly significant for all the characters studied, indicating the presence of sufficient genetic variability. Higher estimates of PCV than corresponding GCV, thereby, revealing that there is slight influence of environment on different traits. The narrow difference between the GCV and PCV values indicated the greater role of genetic factors on the expression of characters than the environmental factors. High heritability in association with high genetic advance as percentage of mean was observed for number of tillers at 120 DAP, number of shoots at 240 DAP, single cane weight, cane length, cane yield and CCS (t/ha) revealed importance of additive gene action and and selection for these characters will be effective for further improvement in cane yield. Cane yield showed significant and positive association with CCS (t/ha), single cane weight, cane girth, cane length, number of millable canes at harvest, number of tillers at 120 DAP and number of shoots at 240 DAP whereas, CCS (t/ha) exhibited positive and significant correlation with cane yield, single cane weight, cane girth, cane length, number of millable canes at harvest, number of tillers at 120 DAP, number of shoots at 240 DAP, brix % at 8th and 10th month, pol % at 8th month and CCS % at 8th month suggests that for improving cane yield and sucrose content direct selection of clones based on positively associated characters will be more fruitful while planning any sugarcane breeding programme. The results of path analysis disclosed that the characters viz., CCS % at 10th month, CCS (t/ha) ,CCS % at 8th month, brix % at 8th month, number of millable canes at harvest, single cane weight, cane girth and number of tillers at 120 DAP recorded positive direct effects on dependent variable i.e. cane yield and the other traits also showed their indirect positive effects on cane yield through these characters, indicating that these characters are the major contributing traits to cane yield in sugarcane. Some sugarcane clones viz. 15-365, 15-1305, 10-31 and 15-1784 were identified in present investigation. These were significantly or numerically superior over best check Co 0238 in terms of cane yield (t/ha), commercial cane sugar (t/ha), pol % at 10th month and CCS % at 10th month. These sugarcane clones needs to be rigorously tested in mutilocational trials to identify suitable genotype to sustain sugarcane productivity.
Open Access
Heterosis and combining ability analysis for yield and fiber quality traits in American cotton (Gossypium hirsutum L.)
(CCSHAU, Hisar, 2020-01) Chakholoma, Maggie; Somveer
The present study was carried out during Kharif season of 2018. The experimental material comprised of 12 lines viz., H 1471, H 1472, H 1480, H 1488, H 1489, H 1508, H 1518, H 1519, H 1520, H 1522, H 1523, H 1098i and 4 testers viz., C 201, C 202, C 210, C 211 and 48 crosses generated by crossing 12 lines with 4 testers in Line x tester design and one standard check hybrid HHH 223. The objective of the study was to determine heterosis of hybrids, combining ability and variances for different traits and identify the best combiners with respect to seed cotton yield and fibre quality. Observations were recorded for 14 characters namely, days to 1st flower, plant height (cm), number of monopods/ number of sympods per plant, number of bolls/plant, bolls weight (g), seed index (g), ginning out turn (%),) lint index, seed cotton yield per plant (g), uniformity index, fibre strength, upper half mean length and micronaire value. Ten crosses recorded a magnitude of heterosis for seed cotton yield per plant of above 40 percent. The highest economic heterosis for seed cotton yield was observed by the cross H 1489 x C 210 which also exhibited highest per se performance and significant sca effects for important characters; number of bolls per plant and boll weight. The variance gca/sca ratio depicted predominance of non-additive gene action for days to first flowering, number of bolls per plant, boll weight, seed index, seed cotton yield per plant, fibre strength, uniformity ratio, upper half mean length and micronaire value. The characters lint index, and number of monopods per plant revealed non- additive and additive gene action while plant height, number of sympods per plant and GOT were pre dominantly controlled by additive gene action. The lines H 1471, H 1472 H 1480 and H 1518 and the tester C-201 were found to be the best combiners and be considered for future breeding programme. The cross combination H 1480 × C 201 recorded highest significant SCA effects for seed cotton yield per plant and also exhibited significant economic heterosis for important characters. H 1098i × C 211 recorded significant SCA effects and economic heterosis for seed cotton yield. Hence, should be considered for further testing over location and environments.

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