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Subject: Genetics and Plant Breeding × End date: 2006 × Start date: 2006 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Studies on genetic diversity in cotton for cotton leaf curl virus disease by using RAPD technique
    (CCSHAU, 2006) Minakshi; Luthra, O.P.
    Cotton is highly priced and economically important crop in the world production and trade. Cotton leaf curl virus (CLCuV) disease is a major threat to cotton production. Therefore, this study was conducted to prepare DNA fingerprint database of 15 cotton genotypes and to assess genetic diversity among them by using random amplified polymorphic DNA (RAPD) primers. Fifteen RAPD primers were used to assess molecular polymorphism in 15 cotton genotypes. A total 135 amplified products were observed of which 16 were monomorphic and 119 polymorphic. Average polymorphism across the 15 genotypes was 87.5 per cent and a significant correlation (0.93, p < 0.01) was observed between total number of bands and number of polymorphic bands. Some of the primers gave unique bands for specific genotypes which further can help in identification of genotypes. Fifteen genotypes were grouped into two main groups based on polymorphism profile generated by using NTSYS-PC programme. One is of resistant genotypes (exception H-1098, a susceptible genotype) and other group is of susceptible genotypes at a similarity coefficient of value 0.555. These two major groups further divided into various subgroups according to similarity between them. HS-182 (S) and HS-6 (S) were most diverse at a similarity coefficient of value 0.510 and 0.520, respectively, merged with other susceptible and resistant genotypes at a similarity coefficient of 0.555. Laxmi (R) and Laxmi (R) x LRA-5166 (R) (F1 hybrid) showed maximum similarity i.e. 85 per cent and HS-182 (S) and HS-6 (S) x H-1098 (S) (F1 hybrid) showed minimum similarity i.e. 40 per cent. Thus, RAPD primers detected high level of polymorphism and fingerprint data generated in this study can be used to improve the resistance among cotton varieties for cotton leaf curl virus disease.