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Theses

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2018 - 20192
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Subject: Genetics and Plant Breeding × Author: Pooja × Start date: 1992 × Type: Thesis ×
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    ThesisItemOpen Access
    Genetic divergence analysis for morphological and biochemical traits in Desi cotton (Gossypium arboreum L.)
    (CCSHAU, 2019) Pooja; Pundir, S.R.
    The present investigation entitled “Genetic divergence analysis for morphological and biochemical traits in Desi cotton (Gossypium arboreum L.)” was carried out at the Research area of Cotton Section and Biochemistry Laboratory, Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar (Haryana, India) during Kharif 2017-18. The observations were recorded for different quantitative and biochemical characters to assess the genetic diversity in 60 Desi cotton genotypes. The GCV and PCV were observed high for seed cotton yield /plant, plant height (cm), number of bolls /plant, lint yield /plant (g), and protein content (%). High heritability was observed for almost all the characters studied except number of monopods /plant. The highest genetic advance as percent of mean was recorded for traits like lint yield /plant (g), seed cotton yield /plant and gossypol content (%). Hence, on the basis of highest value for component of variation and variability parameter direct selection for traits would be most effective. Seed cotton yield /plant had a positive and highly significant correlation with trait like number of monopods /plant, number of bolls /plant, boll weight (g), lint yield /plant (g), seed index and lint index at genotypic level indicated that there was strong inherent association between these traits. On the basis of cluster analysis sixty Desi cotton genotypes were grouped into seven clusters, indicated the presence of substantial genetic diversity in the evaluated germplasm lines/genotypes. In this context, the highest intra-cluster distance was observed in cluster V and lowest in cluster II. The maximum inter-cluster distance were showed between clusters II and IV, whereas, the minimum inter cluster distance was noticed between cluster III and VII, followed by clusters I and II. The genotypes of cluster III viz; FFS-1, Garovilli, H 476-5, HD 10, HD 3 and HD 20 were best performing. Based on study of morphological genetic diversity, the genotypes viz., BH 92, DC-93, HD 5278, FFS-9, BH 102, AC 33, HD 351, HD 372, BH 41, N-W-1, HD 392, G 20 and G 23 showed sufficient amount of genetic diversity for yield and its component traits which may be used in cotton breeding programmes for further improvement.
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    ThesisItemOpen Access
    Molecular analysis of yr genes in wheat (Triticum aestivum L. em.Thell)
    (CCSHAU, 2018) Pooja; Dhanda, S.S
    The present investigation entitled “Molecular analysis of Yr genes in wheat (Triticum aestivum L. em.Thell)”was conducted to evaluate 210 recombinant inbred lines of bread wheat to identify Yr genes using SSR markers, estimate variability and associations among the various traits. Medium to high values of GCV, PCV, heritability and genetic advance as percent of mean for grain yield per plant and biological yield in both years indicating a high scope of selection for these traits followed by 100-grain weight, number of spikelets per ear, harvest index, days to heading and days to maturity. Grain yield was significantly and positively correlated with biological yield, harvest index, number of tillers per plant, ear length, number of spiklets per ear, plant height and number of grains per ear, whereas negative and significantly correlated with days to heading in both years.Biological yield and harvest index had high direct and indirect effects on grain yield per plant over the years, indicating the true relationships with grain yield, in this set of material. Fifteen, out of 70 SSR markers used were found polymorphic in parental genotypes and in RILs. The gene Yr7, Yr18, Yr26, Yr29, Yr36, Yr47 and Yr53 were linked to yellow rust resistance in the present investigation. The RIL No. 52 with 4 Yr genes (Yr7,Yr36,Yr47,Yr53) had better performance for the traits loaded on PC-2, namely grain yield per plant, biological yield and number of tillers per plant and showed 4 Yr genes (Yr7,Yr36,Yr47,Yr53). This line was also better than overall mean for grain yield and its components. The lines viz, RIL No. 13, 24, 31, 120, 121, 134 showed a desirable combination of the traits loaded on PC-1 and PC-2, namely grain yield per plant, biological yield and number of tillers per plant and complemented with disease resistance. Among these lines, RIL No. 24, 121 had 2 Yr genes (Yr18,Yr26, Yr7,Yr47). The RIL No. 117, 25, 12, 15 had better performance for the traits loaded on PC-1, namely grain yield, biological yield and ear length and showed 2 Yr genes out of Yr7,Yr47, Yr18,Yr36, Yr26,Yr29, Yr26,Yr29 observed in the present study. The RIL Nos. 12, 15, 25 were also showed better performance than mean values in terms of grain yield and its components.