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  • ThesisItemOpen Access
    Studies on molecular characterisation, heterosis and combining ability in forage sorghum [Sorghum bicolor (L). Moench]
    (CCSHAU, 2017) Dehinwal, Ashok Kumar; Pahuja, S.K.
    The present study was undertaken to estimate economic heterosis, combining ability with the objective of exploring possibilities of its commercial utilization and stability analysis in four different environments. The material for the present investigation comprised of twenty four forage sorghum hybrids developed between six female parents (lines) and four male parents (testers) in line x testers mating design alongwith two standard checks (SSG 59-3 and MFSH 4). The crosses were made at research area of forage section, CCS HAU, Hisar during the kharif season of 2014-15. Hybrids and parents were evaluated at two locations i.e. research area of forage section, CCSHAU, Hisar and RRS Uchani, Karnal with two dates of sowing (Early and late sowing) during the kharif season of 2015-16. All the thirty six genotypes were grown in a randomized block design with three replications in a two-row plot of 4.0 m length. Data on five randomly taken plants from each genotype in each replication were recorded on different quantitative characters viz. Days to 50% flowering, plant height, number of tillers per plant, leaf length, leaf breadth, stem diameter, green fodder yield, dry fodder yield, and different quality characters viz. TSS content, protein content, protein yield, IVDMD [(in vitro dry matter digestibility), dry matter digestibility (DDM) and HCN content in all the four environments. All data on these quantitative and quality characters were recorded at first cut (55 days after sowing) and second cut (45 days after first cut) separately in all the four environments. Out of 44 SSR primers twelve showed polymorphism. The NTSYS-PC UPGMA cluster tree analysis led to the grouping of ten parental genotypes into two major clusters at similarity index 0.60 which were further divided to sub cluster I, II, and III. Early sowing at hisar (E1) and karnal (E2) were better environments for green and dry fodder yield than late sowing at hisar (E3) and karnal (E4). Hybrids 465A × HJ 513, 467A × G 46 and 9A ×IS 2389 recorded maximum green fodder yield. These hybrids were also good for dry fodder yield, leaf length, leaf breadth, protein yield, IVDMD and DDM. The combining ability analysis revealed that non- additive variance was more than additive variance in the expression of all the traits. Among female parents, 9A and 14A and among male parents, HJ 513 and G 46 were the good general combiners. Hence these may be used for future breeding. Hybrids 465A × G 46, 467A × IS 2389 and 31A × IS 2389 for green fodder yield and dry fodder yield were found stable, average responsive and suitable for all the test environments.