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2019 - 201912020 - 20231
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Subject: Genetics and Plant Breeding × Author: Deepak Kumar × Start date: 2017 ×
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    ThesisItemOpen Access
    Morphological, biochemical and molecular characterization of Asiatic cotton (Gossypium arboreum L.)
    (CCSHAU, Hisar, 2023-01) Deepak Kumar; Sangwan, Omender
    The present investigation “Morphological, biochemical and molecular characterization of Asiatic cotton (Gossypium arboreum L.)” was carried out at Chaudhary Charan Singh Haryana Agricultural University, Hisar in kharif 2019-2020 and 2020-21 with the objective of estimating diversity of 150 genotypes of desi cotton. Variation among genotypes was present for hypocotyl pigmentation, leaf colour, leaf shape, leaf hairiness, flower stigma, leaf nectaries, stem hairiness, boll weight, petiole pigmentation, flower petal colour, boll shape, prominence of tip, plant height, boll opening, seed index, ginning percentage, fibre length, fibre strength and fibre fineness. Analysis of variance revealed presence of variability for all the quantitative traits among the genotypes other than number of locules per boll and seed index. GCV and PCV were high for number of bolls per plant and seed cotton yield, medium for plant height, number of monopods, single boll weight, gossypol content and these parameters were found low in days to first flower, seed index, GOT (%), oil content, fibre length, fibre strength), fibre uniformity, fibre maturity and fibre fineness. High heritability coupled with high genetic advance was observed for most of the traits viz. boll weight, plant height, number of monopods per plant, seed index, oil content, number of bolls per plant, fibre fineness, fibre strength and seed cotton yield per plant. This shows the dominance of additive gene action behind the traits hence direct selection will be effective for these traits. Correlation studies showed that seed cotton yield per plant was significantly and positively correlated with all the traits except days to first flower, plant height and negatively correlated with biochemical and fibre quality traits. Thus, improving other traits, seed cotton yield per plant will also increase. Fibre quality traits like fibre length is significantly positive correlated with fibre strength but negative correlated with seed cotton yield thus suggests that quality and quantity cannot be improved simultaneously one has to be compromised. Whereas, improving fibre length and fibre strength at a time is possible as they are in positive relationship. Genetic diversity analysis was done using 100 SSR markers out of which 66 were found polymorphic with average number of alleles as 2.73 per locus. FFS15 and HD 328 or HD 551 and HD 544 showed 98% similarity index, which means they are the most similar genotypes and have similar genetic background whereas genotypes namely Vira 6 and BHO 5 VIII were the most dissimilar genotypes.
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    ThesisItemOpen Access
    Combining ability and molecular diversity analysis in bread wheat (Triticum aestivum L.)
    (CCSHAU, 2019) Deepak Kumar; Panwar, I.S.
    The present study was carried out to draw out information on the nature of gene effects for yield and its components, genetic variability and assesment of genotypic variability among the parents using SSRs markers in wheat (Triticum aestivum L. em. Thell). The experimental material comprised nine genotypes viz. HD2967, HD3086, HD3059, Raj3765, WH711, WH283, WH1184, WH1105 and WH1124. A diallel set of 9 x 9 was prepared by crossing these genotypes in all possible combinations excluding reciprocals. Parents and their F1 generations were planted during rabi, 2017-18 in RBD (randomized block design) with three replications. The observations were recorded on five randomly selected plants in parents and F1s for thirteen traits. Combining ability analysis was performed by utilizeing method 2, Model-I of Griffing (1956) which indicated that the mean squares due to GCA and SCA were significant for all the thirteen traits. The magnitude of GCA component (gi2) was higher than SCA component (sij2) for plant height and peduncle length, indicating that these traits were mainly under the control of additive genetic variance. An overall appraisal of GCA effects revealed that HD3059and HD 2967 were good combiners for the most of traits. High GCA effects are related to additive gene effects or additive x additive interaction effect which represent the fixable genetic component of variation. For grain yield the crosses, HD2967 × Raj3765, HD2967 × WH711, HD2967 × WH1184, HD3086 × Raj3765, HD3086 × WH711were identified as promising on the basis of their high per se performance, average heterosis and heterobeltiosis and with high significant SCA effects. A total of 56 alleles were detected in and number of alleles per locus ranged from 1-4 with an average of 2 alleles per locus. NTSYS-pc based two dimensional PCA analysis showed scattering of the parents. Similarity coefficient among all the nine genotypes ranged from 0.62 to 0.85. Similarity coefficient revealed WH711 and HD3059 were the most diverse parents among the nine with low similarity index 0.62. WH1124 and WH1184 were showing highest similarity among the nine and highest similarity index 0.85. The findings of genetic similarity coefficient analysis illustrated that wide genetic diversity was present among parental genotypes which was used for making crosses.