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ThesisItem Open Access Study of biochemical and histological alterations due to some aquatic pollutants in freshwater fish Cirrhinus mrigala(College Of Basic Sciences And Humanities Chaudhary Charan Singh Haryana Agricultural University Hisar, 2003) Sharma, Mukta; Jain, K. LThesisItem Open Access Ectoparasitic diseases of coltorable species of fresh water fIshes(College Of Basic Sciences And Humanities Chaudhary Charan Singh Haryana Agricultural University Hisar, 2003) Barman, Anindya Sundar; Yadava, N. KThesisItem Open Access Culture and growth performance of Mugil cephalus (Linn.) and Chanos Chanos (Forsskal) in underground saline water(College of Basic Sciences and Humanities Chaudhary Charan Singh Haryana Agricultural University Hisar, 2003) Barman, Utpal Kumar; Garg, S. KThesisItem Open Access Effects of Cobalt toxicity in some fresh water fishes(College Of Basic Sciences And Humanities Chaudhary Charan Singh Haryana Agricultural University Hisar, 2002) Kumar, Pawan; Gupta, R. KThesisItem Open Access Studies on effects of Lead toxicity in some fresh water fishes(College Of Basic Sciences And Humanities Chaudhary Charan Singh Haryana Agricultural University Hisar, 2002) Budhwar, Dharmender Singh; Gupta, R. KThesisItem Open Access Effect of probiotics, mineral supplementation and biofertilizers on growth performance of some euryhaline fish species in inland saline groundwater(CCSHAU, 2006) Nalle Datta A.; Garg, S.K.In this thesis an attempt has been made to study the role of microbial fertilizers in pond productivity, role of probiotics, Lactobacillus sporogenes and minerals (Ca-P) supplementation on growth performance and some aspects of nutritive physiology of two euryhaline fish species. The thesis is divided into three parts. Part I describes the role of Azotobacter chroococcum and Gluconacetobacter diazotrophicus 35-47 in nitrogen fixation and phosphate solubilization in inland saline ground-water ponds stocked with milkfish, Chanos chanos. Studies have shown significantly (P<0.05) high values for alkalinity, kjeldahl's nitrogen, NO3-N, turbidity, pigment concentration and fish growth in ponds inoculated with co-culture (Mac-27+ PS-21), followed by Mac-27 and PS-21, o-PO4 concentration was significantly (P<0.05) high both in PS-21 and in mixed culture inoculated ponds. Alkanity, hardness, TDS and chlorophyll a concentration were significantly (P<0.05) higher in ponds inoculated with high temperature tolerant mutant of A. chroococcum, while total kjeldahl nitrogen, NH4-N, NO2-N, o-PO4 concentration and fish growth were high in ponds inoculated with salinity tolerant strain of G. diazotrophicus. Not much variations in DO concentration were observed among different treatments. Irrespective of bioinoculants, a significant (P<0.05) increase in viable counts were observed upto day 7 after inoculation, and thereafter a significant decline in microbial population was observed. To study the effect of Lactobacillus sporogenes (Probiotics) on growth performance of Oreochromis niloticus, four diets containing varying concentration (0.25, 0.5, 0.75 and 1.0g 100 g-1 of diet) of probiotics were formulated. A diet without supplementation of probiotics was also formulated which served as control diet. All diets contained about 40% of crude protein. Significantly highest growth performance, carcass protein, apparent protein digestibility, nutrient retention (PER, GPR, GER and APD), digestive enzyme activity were observed in the group fed diet containing probiotics at a concentration of 0.75 g, 100 g-1 of diet. Excretion of metabolites remained low, while the values of VSI and HSI remained high at this treatment. Muscle glycogen and liver glycogen were also low, while the values of muscle protein were high in fish fed diet-3 containing probiotics at a concentration of 0.75g, 100g-1 of diet. These studies indicate that supplementation of diets with appropriate concentration of probiotics can be a useful tool in the utilization of supplementery diets in aquaculture. An experiment was carried out with Chanos chanos and Oreochromis niloticus to determine their phosphorus requirements and its interaction with dietary calcium. Ten isoenergetic and isonitrogenous diets were prepared using basal ingredients (groundnut oilcake, processed soybean, rice bran and wheat flour etc.) containing various concentration of phosphorus as potassium dihydrogen orthophosphate (0.25g, 0.375g and 0.5g 100g-1 of diet) and calcium as calcium chloride (0.5g, 0.75g and 1.0 g 100 g-1 of diet). These concentrations resulted in varying Ca-P ratios (1:1, 2:1, 3:1, 3:2, 4:1, 4:3, 8:3). Calcium and phosphorus concentrations in the water at 10 ppt were 425 mg and 0.03 mg l-1 of water, respectively. Irrespective of the fish species, significantly (P<0.05) high growth performance (growth per cent gain in body weight, SGR and final length, APD, GPR, GER and PER were observed in the groups which were fed on a diet containing calcium and phosphorus at a concentration of 0.5g 100g-1 of diet in the ratio of 1:1. Low FCR and excretion of metabolites (N-NH4, o-PO4) and significantly (P<0.05) highest digestive enzyme activity were also observed in fish fed on this concentration. Low muscle and liver glycogen levels and high muscle protein content coincided with the highest growth performance observed in these studies. VSI and HSI values were also high in fish which had the highest growth performance. Carcass composition also indicated high accumulation of protein. These studies indicated that diets must be supplemented with minerals especially calcium and phosphorus in optimum concentrations and ratios for obtaining the full benefit of supplementary diets.