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  • ThesisItemOpen Access
    Utilization of Karanja Extracts on Cotton Fabric for Microbial Resistance
    (CCSHAU, 2014) Puspa; Punia, Parveen
    The study was conducted to assess the efficacy of karanja extracts on cotton fabric with respect to microbial resistance. Karanja extracts of leaves and seeds in viscous and dry state in two concentrations i.e. 2.5g/l and 5g/l were prepared and four pieces of scoured cotton fabric were impregnated with these four prepared solutions (LEVS, SEVS, SDLP and SDSP) separately.. The antiseptic containing chloroxylenol treatment was taken as standard. Pseudomonas bacterium and Aspergillus fungus were selected for the present study as cotton is susceptible to their growth. To study the effect of all karanja extract treatments and antiseptic containing chloroxylenol (standard) treatment, the treated samples were tested for microbial (bacterium and fungus) resistance activity quantitatively by AATCC-100 test method before and after washing of samples. Fibres for microscopic examination were taken from that portion of the tested samples for tensile strength, from where the fabric was torn out during testing. The percent change in weight, thickness, bulk, bending length, flexural rigidity and elongation of all treated samples were increased after treatment. The increase in these physical properties of treated samples was found with increase in extracts concentrations while air-permeability, moisture regain and tensile strength decreased. After washing, weight, thickness, bulk, bending length, flexural rigidity and tensile strength decreased whereas there was increase in air-permeability, moisture regain and elongation in all treated samples including antiseptic containing chloroxylenol (standard). After assessment of microbial activity against Pseudomonas and Aspergillus through percent reduction all treatments were found effective against Pseudomonas and Aspergillus after 30 minutes of inoculation. Leaves and seeds extracts treatments in viscous state were more effective as compared to other treatments. All extracts in 2.5g/l concentration were very effective after 30 minutes of inoculation (100%) of Pseudomonas and Aspergillus. The 5g/l concentration were found very effective after 30 minutes of inoculation and on 7th day. Maximum (100%) percent reduction was observed in Pseudomonas growth upto 7th day. After washing it was concluded that leaves extracts in viscous state were more effective than other treatments. However it is needed to retreat the fabric samples after every wash. Leaves extracts in viscous state showed comparable results with the antiseptic containing chloroxylenol (standard). In some cases leaves extracts in viscous state was even more effective than antiseptic treatment. It was further concluded that after inoculation of Pseudomonas and Aspergillus these microbes do not affect the physical properties of the treated samples heavily. It was concluded that percent change in weight, thickness, bulk, bending length, elongation and moisture regain increased after treatments whereas air-permeability, flexural rigidity and tensile strength decreased. Treatments with 2.5g/l concentration were more effective than 5g/l concentration and physical properties of samples inoculated with Pseudomonas were less affected than Aspergillus. After inoculation of Pseudomonas and Aspergillus swelling in the fibre was observed indicating the loosening of the fibre. As the efficacy of karanja leaves extract in viscous state was comparable with antiseptic (taken as standard), it can be used at household level as renewable treatment for imparting resistance against the Pseudomonas and Aspergillus growth.