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Subject: Chemistry × Author: Rajita × End date: 2018 × Start date: 2010 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Phytochemical studies and antioxidant activity of radish (Raphanus sativus L.) and turnip (Brassica rapa L.)
    (CCSHAU, 2017) Rajita; Sushila Singh
    The present study was undertaken for phytochemical studies and antioxidant activity of Radish (Raphanus sativus L.) variety Punjab safed and Turnip (Brassica rapa L.) variety White 4 roots grown at Research Farm of Chaudhary Charan Singh Haryana Agricultural University, Hisar. Chemical parameters viz. crude fibre, crude protein, minerals (Fe, Cu, Zn, Mn), total sugars, reducing sugars, non-reducing sugars were analysed in radish and turnip roots. Crude fibre content was higher in radish (13.61%) than turnip (10.34%), crude protein content was higher in turnip (13.39%) than radish (11.51%), Fe content was higher in radish (54.20 ppm) than turnip (39.26 ppm), Cu content was higher in radish (8.82 ppm) than turnip (5.83 ppm), Zn content was higher in radish (19.30 ppm) than turnip (12.44 ppm) and Mn content was higher in radish (107.10 ppm) than turnip (93.60 ppm). Total sugars, reducing sugars and non-reducing sugars content were higher in turnip 169.11, 159.70 and 9.41(mg/g) than radish 92.95, 90.07 and 2.88 (mg/g), respectively. Phytochemical parameters viz. total phenolics and total flavonoids in radish and turnip were extracted with three solvents namely acetone, ethanol and aqueous. Total phenolics content (mg GAE/g) in turnip was highest in aqueous extract (4.56) followed by ethanol (3.75) and acetone (1.99) extracts. In radish also, total phenolics content (mg GAE/g) was highest in aqueous extract (3.93) followed by ethanol (3.70) and acetone (0.61). Flavonoids content (mg CE/g) in turnip was highest in aqueous extract (2.07) followed by ethanol (0.79) and acetone (0.59). In radish also, flavonoids content (mg CE/g) was highest in aqueous extract (1.06) followed by ethanol (0.48) and acetone (0.25). DPPH free radical scavenging activity (%) increased with increase of concentration levels. In radish, it ranged from 1.01 to 76.25 (IC50 0.73 mg/mL) in aqueous extract at concentration levels 0.1-2.5 mg/mL, 6.53 to 76.51 (IC50 14.67 mg/mL) in ethanol extract and 5.73 to 76.20 (IC50 21.42 mg/mL) in acetone extract at concentration levels 1.0-50 mg/mL, respectively. In turnip, it ranged from 1.31 to 75.32 (IC50 1.26 mg/mL) in aqueous extract at concentration levels 0.1-5.0 mg/mL, 17.42 to 80.59 (IC50 11.30 mg/mL) in ethanol extract and 12.33 to 80.43 (IC50 12.08 mg/mL) in acetone extract at concentration levels 1.0-50 mg/mL, respectively. Among acetone, ethanol and aqueous extracts, aqueous extracts contained higher amounts of total phenolic and flavonoids and also exhibited higher antioxidant activity. Hence, aqueous extracts of radish and turnip roots are better source of antioxidants in comparison to acetone and ethanol extracts.