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ThesisItem Open Access Isolation and characterization of chemical constituents from stem of Shisham (Dalbergia sissoo Roxb.)(CCSHAU, 2016) Parvesh Devi; Sushila SinghThe present study was undertaken for Isolation and characterization of chemical constituents from stem of Shisham (Dalbergia sissoo Roxb.). The plant materials was collected from the university campus CCS, Haryana Agricultural University, Hisar. After this the plant materials were immediately brought to laboratory and were kept at room temperature under shade before processing. D. sissoo is an important medicinal plant and commonly known as sisu, shisham, tahli, jag at different parts of world and belongs to family Fabaceae. The tree grows along the hilly areas and different medicinal use of D. sissoo is like blood diseases, syphilis, dysentery, growth inhibitor, anti-inflammatory, analgesic and antipyretic activities. The column chromatography afforded five compounds named as compound D-1 (tritriacontane), compound D-2 (dalbergichromene), compound D-3 (1,6-dihydro-1,6-dihydroxy-4- methoxydalbergione), compound D-4 (dalbergin) and compound D-5 (2,5-dihyroxydalbergiquinol). The compound D-1 was first time reorted from of Dalbergia sissoo stem. The compound D-1 was obtained as colourless solid on elution with hexane and recrystallized from chloroform, m.p. 69-71 °C and Rf value was found to be 0.45 in benzene: hexane (3:7) solvent. Its molecular formula C33H68 was deduced from molecular ion peak m/z 464.The maximum antifungal activity was found in chloroform fraction 98.23% growth inhibition at concentration 2000 μg/ml, moderate antigungal activity was showen by ethyl acetate and benzene fractions with 88.23% and 80.00% growth inhibition against tested fungi. Methanol fraction of D. sissoo stem showed 72.94% growth inhibition at 2000 μg/ml concentration followed by acetone and hexane fractions exhibited by 70.58% growth inhibition at 2000 μg/ml against Rhizoctonia solani fungi.