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Subject: Chemistry × Author: Ajay Singh × End date: 2021 × Start date: 2021 ×
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    ThesisItemOpen Access
    Studies on vicine-convicine and L-DOPA in different plant parts of faba bean Vicia faba L.
    (CCSHAU, Hisar, 2021) Ajay Singh; Ravi Kumar
    Faba bean is protein rich, however, due to the presence of favism causing agents, vicine and convicine, its utility is hindered. The present study was undertaken to estimate the vicine-convicine and L-DOPA contents in different plant parts of faba bean (Vicia faba L.) genotypes (HT 226421, HT 226419, RF 226451, RF 226443, SAEA 226561, SAEA 226556, VT 218733, VT 218725, VT 218712, VT 218702). In order to use vicine standard for estimation of vicine and convicine, its synthesis was achieved by the condensation of guanidine or urea with the -glycoside of ethyl 2-cyano-2-hydroxyacetate. L-DOPA is used for the treatment for Parkinson’s disease and it has an annual demand of $101 billion. So, L-DOPA, vicine-convicine contents were estimated in different plant parts of faba bean by using HPLC. The average L-DOPA content was maximum in flowers followed by leaves, green seeds, pods, podwalls and stems which was 47.87, 28.06, 27.23, 20.54, 14.59 and 1.82 mg/g, respectively on dry weight basis. The average total vicine and convicine content was lowest in stems followed by flowers, leaves, podwalls, pods, mature seeds and green seeds with 0.27, 0.88, 1.12, 1.21, 7.02, 7.20 and 17.43 mg/g, respectively. Total vicine and convicine content was lowest in green seeds of HT 226419, mature seeds of SAEA 226556 and pods of the variety Vikrant. Hence, the present investigation concludes that faba bean is as a good source of natural L-DOPA. Also, the vicine-convicine concentration in the green seeds is significantly high which upon ingestion can cause favism in the G6PD deficient population.