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Subject: Botany × End date: 2021 × Start date: 2021 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Study on sustainability and survival rate of in–vitro propagated sugarcane under green house conditions
    (CCSHAU, Hisar, 2021-09) Shweta; Tallapragada, Sridevi
    The investigation was carried out to study the effect of different energy sources and gelling agents on in vitro multiplication and rooting response on sugarcane cultivar Co-89003. For in vitro multiplication plant cultures were inoculated on MS media supplemented with various sugars (fructose, sucrose, glucose, table sugar and maltose) and gelling agents (agar-agar, agarose, gelrite, guargum and isabgol). The maximum shoots (11.5±0.23 and 9.7±0.23) were observed on medium enriched with table sugar and agrose, respectively. And the maximum shoot-length (7.1±0.39 cm and 7.1±0.51cm) was noticed in sucrose and agar –agar media on after 28th day of inoculation. The very good growth/ rooting (+++++) was observed on ½ MS media supplemented with sucrose and gelrite. Then well rooting plantlets were transplanted into pots containing various compositions of soil, sand, vermicompost, FYM, coco pit and perlite for acclimatization. The maximum and survival (100%) were observed in four different potting mix including sand : soil : FYM, Sand : Soil : Vermicompost, Sand : Soil : FYM : Vermicompost and Coco pit : Vermicompost : Perlite under green house conditions. Hardened plantlets were successfully transferred to the field conditions
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    ThesisItemOpen Access
    Evaluation of antagonistic effect of cell free culture filtrates of Rhizoctonia sp., anastomosis groups/sub-groups infecting Zea mays
    (CCSHAU, Hisar, 2021-08) Sumit; Vimla Singh
    The present study entitled “Evaluation of antagonistic effect of cell free culture filtrates of Rhizoctonia sp., anastomosis groups/sub-groups infecting Zea mays” was conducted during Kharif season of 2020 at CCSHAU, Regional Research Station Uchani, Karnal. Maize (Zea mays L.) is the most economically important cereal crops with a promising yield potential and industrial importance. Banded leaf and sheath blight (BLSB) disease caused by Rhizoctonia solani f.sp. sasakii is a devastating stress on Maize and which leads to huge monetary losses to the farmers. Single hypha tip culture of the isolates viz., Rs-Khp (Kohlapur), Rs-Rch (Ranchi), Rs-Bhw, (Bhuvneshwar), Rs-Kau (Kaul) and Rs-Lck (Lucknow) maintained on PDA plates and characterized for virulence profiles based on cultural, morpho metric and pathogenic variability. The R. solani isolates Rs-Khp, Rs-Kau and Rs- Lck were found to be virulent and comparatively more aggressive than isolates Rs-Rch and Rs-Bhw. Hyphal anastomosis studies showed that, weaker interaction of less virulent isolates with the more virulent isolates. Investigations on cross protective effects of cell free culture filtrates of weaker isolates in suppressing the virulent isolates was examined at dilutions 15% and 25%. Seeds pre-soaked in 15% dilution of culture filtrate showed better results in terms of reduction of severity of symptoms by 25%. However, satisfactory results with 25% dilution were not obtained. The findings reveal that culture filtrate of weaker isolated can serve to reduce yield losses due to severe isolates and such studies may be further undertaken at field level. Further, insight of mechanism of disease suppression may be studied to devise more accurate technique for minimizing losses due to BLSB.