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Study on sustainability and survival rate of in–vitro propagated sugarcane under green house conditions
(CCSHAU, Hisar, 2021-09) Shweta; Tallapragada, Sridevi
The investigation was carried out to study the effect of different energy sources and gelling agents on in vitro multiplication and rooting response on sugarcane cultivar Co-89003. For in vitro multiplication plant cultures were inoculated on MS media supplemented with various sugars (fructose, sucrose, glucose, table sugar and maltose) and gelling agents (agar-agar, agarose, gelrite, guargum and isabgol). The maximum shoots (11.5±0.23 and 9.7±0.23) were observed on medium enriched with table sugar and agrose, respectively. And the maximum shoot-length (7.1±0.39 cm and 7.1±0.51cm) was noticed in sucrose and agar –agar media on after 28th day of inoculation. The very good growth/ rooting (+++++) was observed on ½ MS media supplemented with sucrose and gelrite. Then well rooting plantlets were transplanted into pots containing various compositions of soil, sand, vermicompost, FYM, coco pit and perlite for acclimatization. The maximum and survival (100%) were observed in four different potting mix including sand : soil : FYM, Sand : Soil : Vermicompost, Sand : Soil : FYM : Vermicompost and Coco pit : Vermicompost : Perlite under green house conditions. Hardened plantlets were successfully transferred to the field conditions