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Wide hybridization and embryo rescue for pre breeding in Indian Mustard
(CCSHAU, Hisar, 2022-10) Anjali; Lakra, Nita
The present study was conducted to develop hybrid between Brassica juncea cv. RH 725 taken as a female parent and Sinapis alba genotype DRMR 2183 taken as a male parent with the help of embryo rescue because hybrid embryo usually abort at early stages. The pod setting was 40%. The hybrid siliquae were intermediate in size with respect to the parent plant with an average length of 3.44 cm. The average length of siliquae in B. juncea cv. RH 725 was 4.85 cm and in Sinapis alba the average length was 1.92 cm. Developing ovules were used as an explant and cultured on Murashige and Skoog‟s medium supplied with optimum concentration of different growth regulators. Maximum no. of ovules germinated after 25-30 days of inoculation. Maximum shooting response (48.33%) was observed on MS medium supplemented with MS + BAP (2.5mg/l) + CH (0.5g/l) and maximum rooting response was observed on MS + IAA (0.5 mg/l) i.e. 57.17%. After hardening in a mixture of sterilized sand: soil (1:1), 22% plants successfully moved to green house.