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Subject: Biotechnology and molecular Biology × Author: Kamboj, Aarti × Start date: 1996 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Molecular characterization for targeting foliar blast disease in pearl millet [Pennisetum glaucum (L.) R. Br.]
    (CCSHAU, 2023-04) Kamboj, Aarti; Yashveer, . Shikha
    In the present investigation, 60 pearl millet genotypes were evaluated at experimental field of Bajra Section, CCS HAU, Hisar during kharif season, 2021 for morpho-phenological traits & blast disease and the study of genetic relatedness among them using microsatellite markers. Highly significant mean sum of squares were obtained due to the genotypes for all the traits studied. The traits viz., plant height, ear length, ear diameter, leaf blade width, effective number of tillers per plant, 1000-grain weight, dry fodder yield per plant and grain yield per plant exhibited high heritability (>60%) and high genetic advance as percent of mean (GA%M) (>20%) whereas, the traits ear length, leaf blade width, effective number of tillers per plant, dry fodder yield per plant and grain yield per plant exhibited high Genotypic Coefficient of Variation (GCV) (>20%) and Phenotypic Coefficient of Variation (PCV) (>20%). Correlation coefficient analysis revealed high significant positive correlation coefficients of grain yield with traits viz., plant height, ear diameter and dry fodder yield per plant. As per blast screening, 43 genotypes were found resistant to the disease while 17 genotypes were found susceptible. In order to develop blast resistant hybrids, selected resistant lines were crossed with elite lines of pearl millet and out of the 34 hybrids developed, 32 were found resistant to blast. The increased activities of Phenylalanine Ammonia Lyase (PAL), Peroxidase (POX) and Lipoxygenase (LOX) in the resistant hybrids further confirmed the disease scoring results