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Subject: Biotechnology and molecular Biology × Author: Chaudhary, Rinku × Start date: 1980 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Dissecting the role of OsRuvB transgene for salt tolerance in transgenic chickpea (Cicer arietinum L.)
    (CCSHAU, Hisar, 2023-03-15) Chaudhary, Rinku; Kharb, Pushpa
    Chickpea (Cicer arietinum L.), a self-pollinated diploid (2n = 2x = 16), is one of the most important grain legume with a genome size of approximately 740 Mbp. Chickpea is an annual grain crops with global production of 18.7 million tons, including 11.38 million tons in India. Chickpea is highly sensitive to salinity and its growth is highly affected. Soil salinity decreases the productivity of legume crops including chickpea, which is known for its sensitivity towards salinity. About 20 days old T3 transgenic chickpea seedlings were subjected to 100mM NaCl stress. The seedlings were harvested after 72hrs of NaCl stress and stored in RNA later for trancriptome analysis. A total of approx. 154 million raw reads were generated in all four samples with the total raw data of 49.14GB using Illumina Sequencer. 97493out of 214131 transcripts were observed as uniquely annotated, out of which 24767 genes were differentially expressed and highest number of DEGs were identified from membrane part i.e.15332 DEGs. Molecular function annotation of DEGs listed catalytic activity, transcriptional regulation activity, protein kinase activity, binding activity (heme, ADP), protein serine/threonine kinase activity with the highest members. Some significantly upregulated genes like aldehydehyde dehydrogenase, NADH dehydrogenase, histone, glutamine synthase, Calmodulin, Chlorophyll a,b binding protein, , MAPK, proline-rich protein, MYB48, MYB88, ABC transporter, GABA transporter, zinc finger protein and sucrose synthase were observed. These genes are already reported to play an important role in salt tolerance in various crops, but still more investigation is needed to understand the exact mechanism of salinity tolerance imparted by OsRuvB in transgenic chickpea.