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Subject: Biotechnology and molecular Biology × Author: Bisla, Bindu × Start date: 1980 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Estimation of phytosiderophores in wheat-Aegilops addition/substitution lines under iron deficiency
    (CCSHAU, Hisar, 2021-07) Bisla, Bindu; Upendra Kumar
    Wheat is a major cereal crop all over the world in terms of cultivated area and food source. Most elite wheat cultivars, on the other hand, have low grain iron (Fe) content. Over three billion people, mostly women and children, suffer from micronutrient deficiency, mainly Fe deficiency. However, plants have developed systems to ensure that Fe homeostasis is kept precisely regulated at all levels. The roots improve the nutrient availability in the rhizosphere by removing proton and phenolic compounds from their roots into the rhizosphere which is intended to improve the solubility of ferric ions (Strategy I in dicots) or the solubilization of Fe3+ and other metal micronutrients by phytosiderophores (PS) of the mugineic acid (MA) family secreted from roots (Strategy II, in monocots). In the present investigation, two wheat-Aegilops addition/substitution lines (UP HAU-3 and CS) were grown hydroponically under Fe sufficient and deficient condition. Phytosiderophore release in the root exudates was induced under Fe deficiency and was higher for UP HAU-3 as compared to CS. Significantly higher expression of NAS, NAAT, DMAS and Ids3 was measured in UP HAU-3 than CS under Fe deficiency. We can use biotechnological tools and techniques to introduce the gene responsible for phytosiderophores secretion into field crops. It will enhance the crop uptake in nutrient deficient conditions and indirectly crop yield.