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ThesisItem Open Access Production, Purification And Characterization Of Cellulase-Free Xylanase From Microbial Source(Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2005) Kapoor, Ashima; Dhillon, SantoshThesisItem Open Access Bio-molecular characterization of diversified guar [ Cyamopsis tetragonoloba (L.)Taub.] cultivars for galactomannan(Chaudhary Charan Singh Haryana Agricultural University;Hisar, 2005) Sandeep Kumar; Joshi, Udai NarayanThesisItem Open Access Characterization Of Chickpea, Pigeonpea And Mungbean Rhizobia By Rflp Of Pcr Amplified Products(Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2005) Jagriti; Dhillon, SantoshThesisItem Open Access Antagonistic Effect Of Polyamines On Ripening Related Boichemical Changes In Guava (Psidium Guajava L.)(Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2005) Mondal, Koushik; Malhotra, SarlaThesisItem Open Access Studies On Biochemical And Biological Evaluation Of Potato(Solanum Tuberosum L.) Tubers(Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2005) Kalim, Shahina; Sood, Dev RajThesisItem Open Access Biochemical Evaluations Of Aromatic And Non-Aromatic Genotypes Of Rice ( Oryza Sativa L.)(Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2005) Singh, Akhlash Pratap; Sood, D. R.ThesisItem Open Access Biochemical and molecular characterization of wheat for its strength and extensibility(Chaudhary Charan Singh Haryana Agricultural University;Hisar, 2005) Dawar, Vinamrata; Jain, VeenaThesisItem Open Access Effect of Cd and Cr (VI) on biochemical constituents in oat (Avena sativa L.) during development(CCSHAU, 2005) Tulika; Gupta, KaushalyaAvena sativa L. cv. HJ-8 plants were raised in earthenware pots filled with 5 Kg sand and treated with requisite amounts of Cd (0, 1, 5, 10, 20 and 40 ppm), Cr (VI) (0.1, 0.2, 0.5, 1.0, 2.5 and 5.0 ppm), and Cd:Cr (5:0.1, 5:0.5, 5:1.0, 20:0.1, 20:0.5 and 20:1.0 ppm) respectively in form of CdCl2 and K2Cr2O7. Chlorosis and tip burning started to appear after 10 ppm Cd and 0.5 ppm Cr (VI). At 5 ppm Cr (VI), very reduced plant height and severe chlorosis was observed followed by delayed flowering at 40 ppm Cd and 2.5 ppm Cr (VI) with no inflorescence emergence at 5.0 ppm Cr (VI) application. All the morphological parameters under study showed stimulation at lower doses (upto 5 ppm Cd and 0.5 ppm Cr (VI)) and then inhibited with further rise in heavy metal ion concentration. The fresh weight attained maximum value at 100 DAS and then decreased at maturity. FW and DW also showed a similar trend with increasing heavy metal ion levels. Photosynthetic pigments increased by 40% at 5 ppm Cd and 20% at 0.2 ppm Cr (VI) at 100 DAS and then decreased with increasing levels of Cd and Cr (VI). Chlorophyll b was found to be more sensitive than chlorophyll a under heavy metal toxicity. EC and pH of leaf leachates was found to be similar for all treatments while slight decrease was observed at higher doses. Nitrogen and nitrate nitrogen content was maximum at 5 ppm Cd, 0.1 ppm Cr (VI) and 5:0.1 ppm Cd:Cr combinations. Maximum reduction in Na and K content was observed at 40 ppm Cd, 5 ppm Cr (VI) and 20:1.0 ppm (Cd:Cr) application. However, the stimulation at lower doses was also observed. Crude fibre content was maximum at maturity with maximum reduction of 10% at higher doses of Cd2+ and Cr6+ ions. Sugar content attained maximum value at 100 DAS. Stimulation at lower doses of Cd and Cr was observed for sugar content which was maximum at 5 ppm Cd (43, 25 and 69%), 0.1 ppm Cr (VI) (40,14 and 38%) and Cd:CrThesisItem Open Access Biochemical studies on moth bean [Vigna aconitifolia (Jacq.) marechal] in relation to yellow mosaic virus(CCSHAU, 2006) Arora, Ruby; Joshi, U.N.The present investigation was conducted to study the biochemical changes induced by yellow mosaic virus in resistant (HMO 61 and RMO 225) and susceptible (GMO 9703 and GMO 9704) genotypes of mothbean. Activities of enzymes viz. peroxidase, polyphenol oxidase, phenylalanine ammonia lyase, tyrosine ammonia lyase were increased, whereas the catalase activity was found to decrease in the leaves of inoculated plants of all the four genotypes as compared to the uninoculated plants. Peroxidase activity increased markedly in the susceptible genotypes over the resistant genotypes of inoculated plants. The activities of polyphenol oxidase showed increasing trend in the inoculated leaves of resistant genotypes as compared to the resistant genotypes of uninoculated ones. In the inoculated plants, the phenolic compounds viz. total phenols, flavanols and ortho-dihydric phenols were found to be higher in the resistant genotypes of inoculated plants. Among the structural carbohydrates, no regular trend was observed in NDF and silica content while the contents of ADF, cellulose and lignin increased significantly in the plants inoculated with YMV. The hemicellulose content was found to decrease after inoculation with the pathogen. SDS-PAGE indicates that polypeptides of molecular weight 64.27 KD in seed, and 63.10, 100 and 89.13 KD at vegetative, flowering and grain filling stage in leaves might play a definite role in defense mechanism against YMV in mothbean. The results indicate that the differences between tolerance and susceptibility of host are quantitative as well as qualitative so defense against a pathogen entails major changes in the biochemical components of the plant. A probable biochemical mechanism for defense against YMV in mothbean was suggested.
