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Theses

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2017 - 201912020 - 20231
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Subject: Biochemistry × Author: Vikram Singh × Start date: 2010 × Type: Thesis ×
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    ThesisItemOpen Access
    Development, characterization and efficacy of bionanocluster of indole acetic acid and cobalt chloride on seed germination and yield in Cotton (Gossypium hirsutum L.)
    (CCSHAU, Hisar, 2023-09) Vikram Singh; Mandhania, Shiwani
    In the present investigation, Co2+ and indole-3-acetic acid were combined to create chitosan- Co2+-IAA BNCs. The mean hydrodynamic diameter, PDI value and zeta-potential of BNCs were recorded as 481.9 nm, 0.536 and +22.00 mV, respectively. Effect of seed priming with 100 ppm BNCs and four time foliar application of 100 ppm BNCs on seed germination, seedling growth, and boll drop was studied in H 1098i genotype of G. hirsutum L. Cotton seeds subjected to BNCs priming displayed higher percent germination and seed vigor index-I (92.14 %) compared to hydroprimed seeds due to elevated activities of glyoxylate cycle enzymes i.e. isocitrate lyase and malate synthase, which increased the conversion of fats to carbohydrates. Four times foliar application of 100 ppm BNCs increased leaf area, plant height, total chlorophyll content, photosynthetic rate, ACC synthase activity, indole-3-acetic acid content, boll number, boll weight ultimately increasing seed cotton yield by 20.37 %. However, ACC oxidase, cellulase, pectinase activities and boll drop significantly decreased compared to control. It is concluded that application of BNCs comprising of chitosan, IAA and Co2+ via seed priming and foliar mode is novel, economical, and environment friendly.
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    ThesisItemOpen Access
    Morpho-physiological and biochemical responses of cotton (Gossypium hirsutum L.) genotypes upon sucking insect-pests infestation
    (CCSHAU, 2017) Vikram Singh; Mandhania, Shiwani
    The four cotton genotypes viz., Bio-100 BG II, GCH 3, KDCHH-9810 BG II and HS 6 were evaluated under field conditions to compare their tolarance levels against the sucking insect-pests. The effect of sucking insect-pests on morpho-physiological biochemical constituents viz., the relative water content, specific leaf weight, total chlorophyll content, electrolyte leakage, leaf area, photosynthetic rate, total phenolic content, total soluble sugar, tannin content, total soluble protein, gossypol content, H2O2 content and activity of enzymes- peroxidase and polyphenol oxidase that could explain some of the varietal tolerance levels were tested. Morpho-physiological and biochemical analysis indicated that the highest relative water content, total chlorophyll content, leaf area and photosynthetic rate was observed in Bio 100 BG-II, highest specific leaf weight was observed in GCH-3 and highest electrolyte leakage was observed in HS-6 genotypes. The lowest relative water content, total chlorophyll content, leaf area and photosynthetic rate was observed in HS-6, lowest electrolyte leakage was observed in Bio 100 BG-II and lowest specific leaf weight was observed in KDCHH 9810 BG-II genotypes. The highest total phenolic content, tannin content and POX activity observed in Bio-100 BG II. Gossypol content, total soluble protein and PPO activity were highest in GCH 3. Total soluble sugar and H2O2 content was highest in KDCHH- 9810 BG II. Total phenolic content, gossypol content and tannin content were lowest in HS 6. Total soluble protein and PPO and POX activity were lowest in KDCHH-9810 BG II. Total soluble sugar was lowest in GCH 3. H2O2 content was lowest in Bio-100 BG II. Sucking pest infestation reduced the total soluble sugar and total soluble protein of all genotypes, while other biochemical constituents increased with sucking insect-pests infestation. These results indicated that Bio-100 BG II is highly tolerant as compare to other genotypes.