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Development, characterization and efficacy of bionanocluster of indole acetic acid and cobalt chloride on seed germination and yield in Cotton (Gossypium hirsutum L.)
(CCSHAU, Hisar, 2023-09) Vikram Singh; Mandhania, Shiwani
In the present investigation, Co2+ and indole-3-acetic acid were combined to create chitosan- Co2+-IAA BNCs. The mean hydrodynamic diameter, PDI value and zeta-potential of BNCs were recorded as 481.9 nm, 0.536 and +22.00 mV, respectively. Effect of seed priming with 100 ppm BNCs and four time foliar application of 100 ppm BNCs on seed germination, seedling growth, and boll drop was studied in H 1098i genotype of G. hirsutum L. Cotton seeds subjected to BNCs priming displayed higher percent germination and seed vigor index-I (92.14 %) compared to hydroprimed seeds due to elevated activities of glyoxylate cycle enzymes i.e. isocitrate lyase and malate synthase, which increased the conversion of fats to carbohydrates. Four times foliar application of 100 ppm BNCs increased leaf area, plant height, total chlorophyll content, photosynthetic rate, ACC synthase activity, indole-3-acetic acid content, boll number, boll weight ultimately increasing seed cotton yield by 20.37 %. However, ACC oxidase, cellulase, pectinase activities and boll drop significantly decreased compared to control. It is concluded that application of BNCs comprising of chitosan, IAA and Co2+ via seed priming and foliar mode is novel, economical, and environment friendly.