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ThesisItem Open Access Response of reactive oxygen species production and antioxidant metabolism to putrescine under water stress in wheat leaves(CCSHAU, 2006) Bhankar, Vinita; Sawhney, VeenaThe reduction in RWC and osmotic potential by water deficit stress in the seedlings of WH147 and UP2338 cultivars of wheat was partially enhanced by putrescine applicaton. The superoxide radicals and H2O2 production was high in water deficit plants, also it resulted in the increased lipid peroxidation, but they were decreased on addition of putrescine to the stressed plants, indicating the repair of cellular membranes. Water deficit stress enhanced the activities of H2O2 scavenging enzymes viz. catalase, ascorbate peroxidase and enzymes of ascorbate-glutathione cycle but activity of superoxide dismutase, a H2O2 producing enzyme, was decreased under stress. Exogenously applied putrescine further elevated the activities of these enzymes along with that of superoxide dismutase in both the cultivars. The level of total and reduced glutatione which was decreased due to water stress was recovered by putrescine application. Redox state of glutathione and ascorbate, disturbed in the stressed plants, was found to recover in putrescine treated stressed plants.