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Subject: Biochemistry × Author: Arora, Ruby × End date: 2006 × Start date: 2002 × Type: Thesis ×
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    ThesisItemOpen Access
    Biochemical studies on moth bean [Vigna aconitifolia (Jacq.) marechal] in relation to yellow mosaic virus
    (CCSHAU, 2006) Arora, Ruby; Joshi, U.N.
    The present investigation was conducted to study the biochemical changes induced by yellow mosaic virus in resistant (HMO 61 and RMO 225) and susceptible (GMO 9703 and GMO 9704) genotypes of mothbean. Activities of enzymes viz. peroxidase, polyphenol oxidase, phenylalanine ammonia lyase, tyrosine ammonia lyase were increased, whereas the catalase activity was found to decrease in the leaves of inoculated plants of all the four genotypes as compared to the uninoculated plants. Peroxidase activity increased markedly in the susceptible genotypes over the resistant genotypes of inoculated plants. The activities of polyphenol oxidase showed increasing trend in the inoculated leaves of resistant genotypes as compared to the resistant genotypes of uninoculated ones. In the inoculated plants, the phenolic compounds viz. total phenols, flavanols and ortho-dihydric phenols were found to be higher in the resistant genotypes of inoculated plants. Among the structural carbohydrates, no regular trend was observed in NDF and silica content while the contents of ADF, cellulose and lignin increased significantly in the plants inoculated with YMV. The hemicellulose content was found to decrease after inoculation with the pathogen. SDS-PAGE indicates that polypeptides of molecular weight 64.27 KD in seed, and 63.10, 100 and 89.13 KD at vegetative, flowering and grain filling stage in leaves might play a definite role in defense mechanism against YMV in mothbean. The results indicate that the differences between tolerance and susceptibility of host are quantitative as well as qualitative so defense against a pathogen entails major changes in the biochemical components of the plant. A probable biochemical mechanism for defense against YMV in mothbean was suggested.