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20232
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Subject: Aquatic Animal Health Management × End date: 2023 × Start date: 2023 ×
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    ThesisItemOpen Access
    Effect of indigenous herbal plants on immunity of common carp (Cyprinus carpio L.) challenge to Aeromonas hydrophila infection
    (CCSHAU, Hisar, 2023-05-22) Pandey, Shivam; Gajender Singh
    This study conducted for 90 days to evaluate the effects of neem and tulsi leaf extracts on immunity and growth parameters of common carp challenged with Aeromonas hydrophila infection. The fishes were fed for 30 days with an experimental diet containing different herbal extracts, including T2 (Basal diet + 5% neem leaf extract), T3 (Basal diet +5% tulsi leaf extract), T4 (Basal diet +5% combined neem and tulsi leaf extract) and T1 (Control diet). The combination of neem +tulsi leaf extract demonstrated the highest antimicrobial activity and minimum inhibitory concentration (0.005%), with the largest zone of inhibition (21 mm). The therapy had a 70% death rate at the maximum dose of 1 ×108 cfu/ml. Overall mean values for all treatments (Mean B) showed a similar trend with T4 having the highest RBCs count (1.68) followed by T2 (1.66), T3 (1.65), and T1 (1.33). All the haematological parameters decreased after infection and WBCs are increased. The mean length gain percentage for all treatments over 30, 60, and 90 days was 13.29%, 14.27%, and 16.01%, respectively. The control group (T1) had the lowest mean weight gain percentage of 72.87%. There were no significant differences observed in the length gain percentage between the different treatments. T4 (5% Neem + Tulsi) had the lowest FCR at all time points, with a mean FCR of 1.95 at 30 days, 6.35 at 60 days, and 2.55 at 90 days. The results also revealed that the treatments applied in T2 (53.33± 3.33%), T3 (53.33± 3.33%), and T4 (70.00± 5.77%) may have a positive impact on the survivability of fish. The mean SGR value for the control treatment (T1) was 0.84, while T2, T3, and T4 had slightly higher mean SGR values of 0.88, 0.87, and 0.90, respectively.
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    ThesisItemOpen Access
    Studies on bacterial diseases of cultured freshwater fishes
    (CCSHAU, Hisar, 2023-07) Verma, Ajay Kumar; Ahalavat, Shikha
    Present study was conducted to know the bacterial diseases of cultured freshwater fishes. It includes a survey in Hisar distinct of Adampur, Agroha, Barwala, Hisar1, Hisar 2, and Hansi 1 block and total 25 villages for ten months (February to December 2022) to assess pond fishery resources and the livelihood status of fish farmers. This indicates that a significant proportion of the study participants use Aquaculture drug for their fish culture (fig.9). The highest percentage of participants (52%) belongs to medium size farmer categories, followed by small (32%) and large (16%) farmers (Fig.8). The survey included 25 fish farmers from 25 village of Hisar (Haryana). In contrast, the vast majority of representing 88% reported no diseases among their fish farming. They are aware about fish diseases and other important parameter related to fish farming. Isolation of bacteria from fish to isolate bacteria from infected fish sample is collected from different parts of the fish, such as the gut, gill and infected regions. The study also highlights the potential of using 16S ribosomal RNA gene as a reliable marker for identifying bacterial species. The isolated bacteria were Aeromonas Enteropelogenes, Aeromonas Hydrophila, and Aeromonas veronii, Aeromonas hydrophila (isolate from Catal, Rohu, Common carp, and Silver carp). . All the bacteria was associated with the different types of diseases including tail rot, fin rot, dropsy and motile aeromonas septicemia etc. according to the symptoms observed on infected fish body.