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  • ThesisItemOpen Access
    Comparative study on the effects of different sheds on the growth performance of Vietnam koi (Anabas testudineus Linn.) fish in biofloc system
    (CCSHAU, Hisar, 2023-06) Sandeep Kumar; Dahiya, Tejpal
    This thesis titled "Comparative study on the effect of different sheds on growth performance of Vietnam koi (Anabas testudineus Linn.) in Biofloc system" aimed to compare the effectiveness of two biofloc systems (with green net and tin shed) in maintaining water quality parameters for successful Vietnam koi fish culture. The study was conducted from June 2022 to December 2022 for six months, comparing the average weight and length gain, survival rate, specific growth rate (SGR), and water quality parameters between the two systems. The study found that both systems were effective in maintaining water quality parameters, but the green net shed biofloc system showed better results in terms of fish growth (110.16 g), nitrite (0.20 mgL-1), floc volume (27.76 mlL-1), alkalinity (208.07 mgL-1), and maintenance of optimum temperature (28.740C). A. testudineus reared under the green net shed biofloc system had significantly higher daily weight gain (0.99 g) and lower feed conversion ratio (1.05) than those under the tin shed biofloc system. The specific growth rate (SGR) was not significantly different between the two systems (1.79 g and 1.69 g for green net and tin shed respectively). The study observed that ammonia levels were higher (0.31 mgL-1) in the tin shed than green net shed (0.27 mgL-1) biofloc system and was within the acceptable range for Vietnam koi farming. The results of this study suggest that the green net shed biofloc system is a better option for successful Vietnam koi fish culture in terms of growth performance and water quality parameters.
  • ThesisItemOpen Access
    Optimum utilization of Delonix regia leaf meal in the diet of Labeo rohita (Hamilton, 1822)
    (CCSHAU, Hisar, 2023-05) Lowanshi, Ashutosh; Gulati, Rachna
    During present investigation, groundnut oil cake and deoiled rice bran were replaced by Delonix regia leaf meal under varying proportions to evaluate their effect on survival, growth, hematological parameters and enzymatic activity of Labeo rohita. On dry matter basis, groundnut oil cake, deoiled rice bran and D. regia leaves contained 42, 13 and 22.12 percent crude protein, respectively. Inclusion of D. regia leaf meal at 60 percent as deoiled rice bran replacement significantly increased the average weight (3.42g), average daily weight gain (0.22g), total weight (123.28g), total weight gain (34.26g), biomass (123.29g), percent increase in length (12.56%) of L. rohita as compared to control (2.61g, 0.17g, 89.07g, 26.13g, 89.07g, 10.26%, respectively). Percent increase in weight, specific growth rate, length of L. rohita was significantly higher at (62.21%, 1.25%,7.11cm) at 100 percent replacement of deoiled rice bran with D. regia leaf meal than at 0 (50.91%,1.14%, 6.06cm) percent replacement diet. The mean values of the average weight, total weight, weight gain of L. rohita were significantly higher in treatment fed with 20 percent inclusion of D. regia as replacement of groundnut oil cake as compared to other treatments and control with 0 percent inclusion of D. regia, respectively. Biomass and average daily weight gain of L. rohita were recorded highest in treatment fed with 50 percent inclusion of D. regia (114.25g, 0.30g) and lowest in the control group (99.42g, 0.16g). D. regia leaf meal diets did not show any adverse effect as 100 percent survival of L. rohita was recorded in all the treatments. Minimum FCR (1.56) was observed in D. regia leaf meal (30%) as replacement of groundnut -oil cake diet and maximum (1.94) in control diet. The protein efficiency ratio (PER) of the diet fed to L. rohita was found to be statistically higher (0.64) in 30 and 20 percent (0.60) D. regia leaf meal as replacement of groundnut -oil cake diet. Replacement of deoiled rice bran with 60 percent D. regia leaf meal gave better results in terms of percent increase in length (12.56%) and specific growth rate (1.25%) as compared to 30 percent D. regia leaf meal replacement of groundnut oil cake (4.47%, 1.08%, respectively). Feed conversion ratio (1.47) and protein efficiency ratio (0.68) were also better in 30 percent D. regia leaf meal replacement of deoiled rice bran than at 30 percent D. regia leaf meal replacement of groundnut oil cake (1.56, 0.64, respectively). Apparent digestibility of D. regia leaf meal diets for 40 and 100 percent replacement of groundnut oil cake and deoiled rice bran was 77.5 and 77.97 percent, respectively. The pH, dissolved oxygen, and temperature remained in the range of 7.03 to 7.40, 6.02 to 6.13 ppm, and 24.66 to 25.56oC during the course of study showing no effect of D. regia diets.Free ammonia in different treatments remained under permissible level (0.05 to 0.09 ppm). Significantly higher total dissolved solids (79.50 to 91.52 ppm) were recorded in D. regia inclusion diets as compared to control diet (75.07 ppm). Haemoglobin and Haematocrit value of L. rohita was lowest in control diet (8.28g/dl, 22.8%) which significantly increased to 9.24 g/dl and 27.8 percent in 50 percent D. regia inclusion as replacement of groundnut oil cake. No significant effect of treatment diets was recorded on red blood cells and white blood cells. Red blood cell and white blood cell counts remained within the range of 2.39 to 3.20 × 106 μl and 17.49 to 17.52×103/μl, respectively. Effect of D. regia leaf meal on enzyme activity of L. rohita showed significantly lower Lipase activity (1.41 μM/mg protein/min) at 50 percent D. regia inclusion. D. regia leaf meal treatment diets did not influence the amylase and protease activity significantly. Amylase activity ranged between 18 to 25.50 μM/mg protein/min whereas, protease activity ranged from 24.21 to 28.59 Fluorescence change/unit. The anti-nutritional factors viz., total phenolic content, total oxalate content, tannin content, phytic acid and total alkaloids in the ethanolic extract of Delonix regia leaf were 31.24 mg gallic equivalents (GAE)/g sample in dry weight, 1.28mg/l, 17.80mg/l, 2.43mg/100g and 26.97 mg/100g, respectively.
  • ThesisItemOpen Access
    Effect of polythene lining on hydrobiological and growth parameters of Litopenaeus vannamei boone
    (CCSHAU, Hisar, 2023-05) Surender Kumar; Gupta, R.K
    The study was conducted to assess the entitled Effect of polythene lining on hydrobiological and growth parameters of Litopenaeus vannamei Boone under two objectives wise as follows:This objective had three replicates and the duration of present study was four months, from 14 June 2022 to 22October 2022.The experiment was conducted for 120 days during the year 2022 in the proposed study will be carried out in College of Fisheries Science and white legged shrimp farms situated in village Thuiyan, District Fatehabad. Pacific white shrimp were fed with commercial feed. The average daily weight (ADW) of shrimp between ponds with and without polythene lining. The findings showed that the shrimp reared in the ponds with polythene lining had an ADW of 0.235 g, while the shrimp from the ponds without polythene lining had a significantly lower ADW of 0.206 g. The feed conversion ratio (FCR) for ponds with polyethylene lining, with a ratio of 1.27 compared to 1.35 for ponds without lining. The difference in weight gain between ponds with and without polythene lining. The polythene lined pond recorded a weight gain of 236185.7%, while the pond without lining recorded a weight gain of 211438.4% . The biochemical oxygen demand (BOD) was observed to be in the range of 0.57 mg/l to 1.14 mg/l in polythene-lined ponds, while in ponds without a lining, it was recorded to be between 0.29 mg/l to 1.26 mg/l.
  • ThesisItemOpen Access
    Effect of stocking density, salinity & Azolla based feed on growth and survival of Indian Butter Fish Pabda (Ompok bimaculatus)
    (CCSHAU, Hisar, 2023-07) Maurya, Mitrasen; Ahalavat, Shikha
    The present study was conducted to know the effect of stocking density, salinity and azolla based feed on growth and survival of Indian butter catfish (Ompok bimaculatus). The experiment was carried out for 45 days (1st experiment), 45 days (2nd experiment) and 60 days (3rd experiment). In first set of experiment, the fingerlings of O.bimaculatus were stocked under three different stocking densities such as 10 fingerlings/tank (T1), 15 fingerlings/ tank (T2) and 25 fingerlings/ tank. Under first experimental study, the highest weight gain, specific growth rate, and length gain were found in O.bimaculatus at a lower stocking density (10fingerlings/tank) with values of 2.95 g, 1.12%, and 1.10 cm, respectively. The lowest FCR was recorded (1.90) in T1 (10fingerlings/tank) and highest (2.58) was recorded in T3 (25 fingerlings/tank). A 100% survival rate was observed at a lower stocking density of 10 fingerlings per tank, while an 88% survival rate was observed at a higher stocking density. In the second objective of my research work, the survival rates were determined to be 100% in T1 (0.5 ppt), 96.66% in T2 (2 ppt), 63.3% in T3 (4 ppt), and 33.3% in T4 (6 ppt). Additionally, it was observed that weight gain was highest at lower salinity levels (0.5 ppt), while the lowest weight gain was recorded at higher salinity levels (6 ppt). fishes growth declined significantly at 4 and 6 ppt (p≤0.05). Under my research work, the effect of different percent of Azolla diet in combination with commercial feed on the growth and survival of O. bimaculatus was observed in the last experiment. fingerlings of O.bimaculatus were reared under four treatment such as T1(Commercial diet), T2( Commercial diet + 20 % Azolla), T3(Commercial diet + 30% Azolla ) and T4(100%Azolla).Under third experimental study, the highest DWG, ADWG and SGR were found in fingerlings in T2( Commercial diet + 20 % Azolla) with values of 0.29 g, 0.31 g, and 1.75% respectively and lowest in T4(100% Azolla ) with values 0.04 g, 0.04g and 0.39 %. FCR was recorded better in T2(1.61) than T1(1.87), T3 (1.96) and T4(6.61). A survival rate of 83.33% to 96.66% was achieved in the third experiment of my research study, even in the control group and with a 100% Azolla diet. A high rate of survival was observed in both the T2 group and the control group.
  • ThesisItemOpen Access
    Effect of different dietary sources on the rearing of Amur carp (Cyprinus carpio haematopterus)
    (CCSHAU, Hisar, 2021-09) Shahi, Sudha; Gajender Singh
    In present study, different types of feed were incorporated in diet included T1 (live feed), T2 (live+formulated feed), T3 (formulated feed) and commercial feed as a control treatment (Tₒ) to assess the effect of different dietary sources on the rearing of Cyprinus carpio haematopterus fry for three months. 180 fry having initial weight and length (.25 gm and 2.08 cm) were recorded and divided into 12 groups having 15 fries in each treatment tank. The growth parameter and water quality parameters were measured at fortnight intervals. The study showed that all the growth parameters were found significantly (p=0.05) increasing throughout the experiment period. The maximum weight and length were observed in T2 treatment i.e. (5.31±0.01) gm and (5.25 ±0.02) cm and minimum weight and length were observed in control treatment Tₒ i.e. (3.37±0.04) gm and (3.10± 0.10) cm. SGR, DWG, and ADG were found maximum in T2. The FCR of (1.30±0.05) was found best in treatment T2. Various physicochemical parameters (DO, pH, electrical conductivity, total alkalinity, total hardness, calcium, magnesium, chloride, and ammonia concentration) were ranged within the tolerance level in the experimental period. At the end of the experiment carcass composition analysis showed maximum protein of (49.67±0.66) % and fat of (25.33±2.33) % in T2 treatment. The result of the experiment depicted that incorporation of a mixed diet of live feed and formulated feed completes the nutrient requirement for the rearing of Cyprinus carpio haematopterus fry